口腔医学研究

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (11): 1188-1192.DOI: 10.13701/j.cnki.kqyxyj.2016.11.019

• 临床研究论著 • 上一篇    下一篇

野黄芩素协同ABT-737抗头颈鳞癌作用的研究

静广平*,吕克文,张晗,吕行   

  1. 哈尔滨医科大学附属口腔医院颌面外科 黑龙江 哈尔滨 150001
  • 收稿日期:2016-05-16 出版日期:2016-11-25 发布日期:2016-11-25
  • 通讯作者: 静广平,E-mail: jinggp2003@163.com
  • 作者简介:静广平(1974~ ),男,黑龙江省大庆市人,副教授,博士,主要从事口腔颌面-头颈肿瘤临床治疗及基础研究。
  • 基金资助:
    黑龙江省教育厅科学技术研究项目(编号:12541294)

Synergistic Anti-tumor Activity of Scutellarein and ABT-737 on Head and Neck Squamous Cell Carcinoma

JING Guang-ping*, LV Ke-wen, ZHANG Han, LV Xing   

  1. The affiliated Hospital of Stomatology, Harbin Medical University, Harbin 150001, China.
  • Received:2016-05-16 Online:2016-11-25 Published:2016-11-25

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摘要： 目的:研究野黄芩素协同ABT-737在体外对人头颈鳞癌HN30细胞的凋亡诱导作用,探讨其可能的作用机制。方法:MTT法检测野黄芩素协同ABT-737抑制细胞增殖作用;流式细胞术检测细胞凋亡; JC-1染色法检测线粒体膜电位变化;western blotting检测细胞凋亡蛋白的表达;利用SiRNA技术沉默基因表达。结果:野黄芩素协同ABT-737在体外对人头颈鳞癌HN30细胞增殖有抑制作用,且呈量效关系;两者可协同诱导HN30细胞凋亡;使线粒体膜电位去极化;下调抗凋亡蛋白Bcl-2、Bcl-XL表达,上调Bax表达;SiRNA抑制Mcl-1表达可以显著增强野黄芩素和ABT-737协同诱导HN30细胞凋亡的作用。结论:野黄芩素在体外可以协同ABT-737诱导人头颈鳞癌HN30细胞凋亡,野黄芩素下调Mcl-1蛋白的表达,抑制Mcl-1对ABT-737抗肿瘤作用的影响,是两者协同作用的机制。

关键词: 野黄芩素, ABT-737, 头颈鳞癌, 协同作用

Abstract: Objective: To investigate the synergistic anti-tumor activity of Scutellarein and ABT-737 on head and neck squamous cell carcinoma HN30 cell line in vitro and to discuss the mechanisms. Methods: Inhibitory effects of ABT-737 and Scutellarein on HN30 cells were detected by MTT assay. Cell apoptosis was analyzed by flow cytometry. Mitochondrial transmembrane potential (ΔΨm) was analyzed by JC-1 staining. The expression of the protein related to apoptosis was quantified by western blot. Genes silencing was conducted by SiRNA. Results: Scutellarein and ABT-737 could synergistically inhibit the proliferation of HN30 cells in vitro in a dose-dependent manner. Scutellarein and ABT-737 could synergistically induce the apoptosis of HN30 cells, induce the Mitochondrial membrane potential depolarization, down-regulate the expression of Bcl-2 and Bcl-XL, and up-regulate the expression Bax. Inhibition of the expression of Mcl-1 induced by SiRNA could significantly enhance the synergistic effect of scutellarein and ABT-737 on HN30 cells apoptosis. Conclusion: Scutellarein and ABT-737 could synergistically induce the apoptosis of HN30 cells in vitro. The expression of Mcl-1 was down regulated by scutellarein and was correlated with the drug resistance for ABT-737.

Key words: Scutellarein, ABT-737, Head and neck squamous cell carcinoma, Synergistic

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