熊果酸对成牙骨质细胞增殖及矿化功能的影响

doi:10.13701/j.cnki.kqyxyj.2016.12.002

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (12): 1229-1233.DOI: 10.13701/j.cnki.kqyxyj.2016.12.002

熊果酸对成牙骨质细胞增殖及矿化功能的影响

王玉琢^1,3,包幸福^1,3,姜欢^1,3,高尚^1,3,刘金凤^2,3,王春晖^1,3,胡敏^1,3*

1. 吉林大学口腔医学院正畸科吉林长春 130021;
2. 吉林大学口腔医学院牙体牙髓病科吉林长春 130021;
3. 吉林省牙发育及颌骨重塑与再生重点实验室吉林长春 130021

收稿日期:2016-03-02 出版日期:2016-12-25 发布日期:2016-12-26
通讯作者: 胡敏,E-mail: humin@jlu.edu.cn
作者简介:王玉琢(1991~ ),女,吉林磐石人,硕士在读,主要从事正畸牙根吸收与修复方面的研究。

Effects of Ursolic Acid on Proliferation and Mineralization of Cementoblasts

WANG Yu-zhuo^1,3, BAO Xing-fu^1,3, JIANG Huan^1,3, GAO Shang^1,3, LIU Jin-feng^2,3, WANG Chun-hui^1,3, HU Min^1,3*

1. Department of Orthodontics, School and Hospital of Stomatology, Jilin University, Changchun 130021, China;
2. Department of Endodontics, School and Hospital of Stomatology, Jilin University, Changchun 130021, China;
3. Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling , Changchun 130021, China.

Received:2016-03-02 Online:2016-12-25 Published:2016-12-26

摘要/Abstract

摘要： 目的:观察熊果酸对鼠成牙骨质细胞系OCCM-30增殖、周期、凋亡及矿化功能的影响。方法:一定浓度熊果酸处理成牙骨质细胞后,cck-8法检测细胞增殖,流式细胞仪检测细胞周期及凋亡,微量酶标法检测碱性磷酸酶活性,茜素红染色法定量及定性检测矿化结节量。结果:熊果酸组(2.5 μmol/L)相比于对照组,细胞增殖率和细胞周期增殖指数升高,早期凋亡率降低,碱性磷酸酶活性和矿化结节量上调。结论:一定浓度熊果酸可促进成牙骨质细胞增殖,G1期百分比降低,抑制早期凋亡,增强碱性磷酸酶活性,促进矿化结节的形成。

关键词: 熊果酸, 鼠成牙骨质细胞OCCM-30, 牙根吸收与修复

Abstract: Objective: To investigate the effect of ursolic acid on murine cementoblasts cell line OCCM-30 in vitro. Methods: Cementoblasts were incubated with ursolic acid at various concentrations. Proliferation was detected using cck-8 assay. Cell cycle and apoptosis were checked by flow cytometry analysis. The activity of alkaline phosphatase was tested by enzyme-linked method on microplate. The mineralization was tested by alizarin red S staining. Results: Compared with the control group, ursolic acid (2.5μmol/L) increased the cell proliferation rate and proliferation index of the cell cycle, reduced the early apoptotic rate, up-regulated the activity of alkaline phosphatase and the quantity of the mineralized nodules. Conclusion: Ursolic acid can promote the proliferation of cementoblast, enhance the activity of alkaline phosphatase, and facilitate the differentiation.

Key words: Ursolic acid , Murine cementoblasts cell line OCCM-30, Root absorption and repair

中图分类号:

R783.5

王玉琢,包幸福,姜欢,高尚,刘金凤,王春晖,胡敏. 熊果酸对成牙骨质细胞增殖及矿化功能的影响[J]. 口腔医学研究, 2016, 32(12): 1229-1233.

WANG Yu-zhuo, BAO Xing-fu, JIANG Huan, GAO Shang, LIU Jin-feng, WANG Chun-hui, HU Min. Effects of Ursolic Acid on Proliferation and Mineralization of Cementoblasts[J]. Journal of Oral Science Research, 2016, 32(12): 1229-1233.

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熊果酸对成牙骨质细胞增殖及矿化功能的影响

Effects of Ursolic Acid on Proliferation and Mineralization of Cementoblasts

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