口腔医学研究

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (12): 1287-1291.DOI: 10.13701/j.cnki.kqyxyj.2016.12.015

• 临床研究论著 • 上一篇    下一篇

两种不同方法使用生物活性玻璃治疗牙本质敏感的体外试验研究

马骞1,吴凤鸣2,罗瑞1,王婕1,陈亚明1*   

  1. 1. 南京医科大学口腔疾病研究江苏省重点实验室,南京医科大学附属口腔医院综合科 江苏 南京 210029;
    2. 南京医科大学附属口腔医院修复科 江苏 南京 210029
  • 收稿日期:2016-05-31 出版日期:2016-12-25 发布日期:2016-12-26
  • 通讯作者: 陈亚明,电话:025-85031913
  • 作者简介:马骞(1984~ ),女,江苏淮安人,博士在读,医师,主要从事口腔生物活性材料研究。
  • 基金资助:
    南京市科技计划项目(编号:201503062)
    江苏高校优势学科建设工程资助项目(编号:2014-037)
    南京医科大学口腔医学院横向合作项目(22011001)

Evaluation of Dentin Desensitizing by Bioglass with Two Different Methods in Vitro.

MA Qian1, WU Feng-ming2, LUO Rui1, WANG Jie1, CHEN Ya-ming1*.   

  1. 1. Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University;
    Department of Polyclinics, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing 210029, China;
    2. Department of Prosthodontics, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing 210029, China.
  • Received:2016-05-31 Online:2016-12-25 Published:2016-12-26

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296

被引次数

3

摘要： 目的:体外比较涂抹法和透明压膜托盘法使用生物活性玻璃脱敏剂在短时间内对牙本质小管的封闭效果。方法:制取56颗离体牙的牙本质片,6%柠檬酸脱矿后按处理方法和处理时间不同随机分为7组,涂抹法1 d、3 d、7 d组(A1、A2、A3),压膜法1、3、7 d组(B1、B2、B3),空白对照组(C)。通过场发射扫描电镜(FESEM)观察比较各组牙本质小管封闭效果及再矿化物深度,X射线能谱分析(EDX)两种方法处理后牙本质表面再矿化物成分。结果:FESEM显示涂抹法和透明压膜托盘法使用生物活性玻璃脱敏剂后均能很好的封闭牙本质小管,牙本质小管内均可见明显矿化物沉积,牙本质小管暴露率A1组和B1组间无显著差异,A2组>B2组,A3组>B3组,差异有统计学意义(P<0.05);空白对照组牙本质小管口开放,周界清晰,牙本质小管暴露率为(19.11±1.27)%。纵剖面观察AB组小管内均有再矿化沉积物。X射线能谱分析(EDX)分析,各组间牙本质表面钙/磷比无显著性差异。结论:透明压膜托盘法在短时间内可以显著提高生物活性玻璃对敏感牙本质小管的封闭效果,与涂抹法相比,封闭效果有显著性提高。

关键词: 封闭效果, 牙本质小管, 透明压膜托盘法

Abstract: To evaluate the effect of bioactive glass desensitizer on dentinal tubule orifice occlusion through smearing or transparent pressure-swage. Methods: Fifty-six dentin slices were selected, and after demineralization, the slices were randomly divided into 7 groups: smearing the bioactive glass for 1 day, 3 days and 7 days (A1, A2, A3), applying the bioactive glass by pressure-swage for 1 day, 3 days and 7 days (B1, B2, B3) and negative control group. Occlusion effect on dentinal tubule orifice was observed via FESEM. Surface mineral content disparity of each dentin slices was analyzed by EDX. Results: FESEM results indicated that dentinal tubules were well occluded after smearing or transparent pressure-swage applying of bioactive glass desensitizer. The difference of dentinal tubule exposure between A1 and B1 was not significant different. The effect of dentinal tubule occlusion was better in B2 and B3 groups than in A2 and A3 groups. EDX results showed that Ca/P ratio on the dentinal surface in each group was not significantly different. Conclusion: Compared with smearing method, trans-parent pressure-swage method could improve the occlusion effect of dentinal tubules by bioactive glass in a short period of time.

Key words: Occlusion effect, Dentinal tubule , Transparent pressure-swage

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