口腔医学研究

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (1): 99-101.DOI: 10.13701/j.cnki.kqyxyj.2017.01.023

• 临床研究论著 • 上一篇    下一篇

柠檬精油对牙周致病菌抑制作用及其安全性初步研究

李唐萍1,边可胤2,张向宇3*   

  1. 1. 广州医科大学附属口腔医院·广州口腔病研究所·口腔医学重点实验室 广东 广州 510140;
    2. 天津市口腔医院 天津 300041;
    3. 天津医科大学口腔医院口腔预防科 300070
  • 收稿日期:2016-07-22 出版日期:2017-01-25 发布日期:2017-01-22
  • 通讯作者: 张向宇,E-mail:xzhang04@tmu.edu.cn
  • 作者简介:李唐萍(1989~ ),女,重庆人,硕士,主要从事儿童口腔方面临床及基础研究工作。
  • 基金资助:
    天津市卫计委科技基金(编号:2011ky16)
    天津市科技发展基金面上项目(编号:10jcybjc13600)

Effect of Lemon Essential Oil on Periodontal Pathogens

LI Tang-ping1, BIAN Ke-yin2, ZHANG Xiang-yu3*   

  1. 1. Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou 510140, China;
    2. Tianjin Stomatological Hospital, Tianjin 300041, China;
    3. Department of Preventive Dentistry, Stomatological Hospital of Tianjin Medical University, Tianjin300070, China
  • Received:2016-07-22 Online:2017-01-25 Published:2017-01-22

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摘要： 目的:探讨柠檬精油对牙周致病菌的体外抗菌活性及对细胞增殖的影响。方法:采用微量液体稀释法测定柠檬精油对Pg、Fn、Aa、Pi的最小抑菌浓度(minimal inhibitory concentration,MIC)及最小杀菌浓度(minimum bactericidal concentration,MBC);以较低浓度的MIC为标准稀释LEO作为实验组,采用MTT法测定柠檬精油对HUVECs的毒性作用,明确抑菌浓度下LEO的安全性。结果:柠檬精油对牙周主要致病菌均有抑菌作用,Pg、Fn、Aa、Pi的MIC分别是9.0 g/L、4.5 g/L、4.5 g/L、9.0 g/L,Aa、Fn的 MBC是9.0 g/L,Pg、Pi的MBC未测得。1/2MIC、1/20MIC浓度的LEO能够抑制人脐静脉内皮细胞的生长,而低于1/200MIC浓度的LEO则对人脐静脉内皮细胞的生长没有影响,其中1/200MIC浓度的LEO作用明显优于0.02%的CHX。结论:体外环境中,柠檬精油对牙周致病菌Pg、Fn、Aa、Pi具有抗菌活性,低浓度应用对机体相对安全。

关键词: 柠檬精油, 牙周致病菌, 最小抑菌浓度, 人脐静脉内皮细胞

Abstract: Objective: To investigate effects of lemon essential oil (LEO) on the growth and adherence of periodontal pathogens. Methods: Microplate dilution assays were performed to determine the antibacterial effect of LEO on Prophyromonas gingivalis (Pg), Fusobacterium nucleatum (Fn), A.actinomycetemcomitan (Aa), and P.intermedius (Pi). MTT method was employed to study the cytotoxic activity of LEO on human umbilical vein endothelial cells (HUVECs). Results: The LEO exhibited moderate antibacterial activity on Pg and Pi (Minimum inhibitory concentration: MIC =9mg/mL) and strong antibacterial activity on Fn and Aa (MIC= 4.5mg/mL). The minimal bactericidal concentration (MBC) was 9.0mg/mL (Fn and Aa). LEO showed no killing effect on Pg and Pi. The LEO’s concentration (<1/200 MIC) revealed no toxicity on HUVECs. Conclusion: LEO has a great potential to be used to treat periodontitis.

Key words: Lemon, essential, oil, Periodontal, pathogen, Minimal, inhibitory, concentration
Human, umbilical, vein, endothelial, cells

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