调牙合对前导增大的大鼠TMJ滑膜中COX-2、iNOS转归变化的研究

doi:10.13701/j.cnki.kqyxyj.2017.04.004

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (4): 362-366.DOI: 10.13701/j.cnki.kqyxyj.2017.04.004

调牙合对前导增大的大鼠TMJ滑膜中COX-2、iNOS转归变化的研究

李雨轩^1,2, 姚天华^1,3#, 白乐康², 谭文宏², 韩会芳², 翟桐³, 肖敏^1,2*

1. 陕西省颅颌面精准医学研究重点实验室陕西西安 710004;
2. 西安交通大学口腔医院修复科陕西西安 710004;
3. 西安交通大学口腔医院综合科陕西西安 710004

收稿日期:2016-09-16 出版日期:2017-04-20 发布日期:2017-04-24
通讯作者: 肖敏,电话:029-87218541
作者简介:李雨轩(1988~ ),女,陕西人,硕士,医师,主要研究方向牙合学与颞下颌关节紊乱病。姚天华(1976~ ),女,河南人,硕士,主治医师, 主要从事口腔综合类研究。
基金资助:
陕西省社会发展科技攻关基金项目(编号:2012k16-10-03)

Effect of Occlusal Intervention on the Expression of COX-2 and iNOS in the Temporomandibular Joint Synovial Membrane of Rat Caused by Increased Anterior Guidance.

LI Yu-xuan^1,2, YAO Tian-hua^1,3#, BAI Le-kang², TAN Wen-hong², HAN Hui-fang², ZHAI Tong³, XIAO Min^1,2*.

1. Key Laboratory of Shanxi Province for Craniofacial Precision Medicine Research, Xi'an 710004, China;
2. Department of Prosthodontics, College of Stomatology, Xi'an Jiaotong University, Xi'an 710004, China;
3. Department of General Dentistry, College of Stomatology, Xi'an Jiaotong University, Xi'an 710004, China.

Received:2016-09-16 Online:2017-04-20 Published:2017-04-24

摘要/Abstract

摘要： 目的:探讨调牙合干预措施对前导增大引起的大鼠颞下颌关节(Temporomandibular joint,TMJ)滑膜中诱导型一氧化氮合成酶(Inducible nitric oxide synthase,iNOS)及环氧合酶-2(Cyclooxygenase-2,COX-2)表达变化的影响。方法:在大鼠上前牙粘结舌侧增大的金属冠,建立前导增大组(C),调牙合组在建立前导斜度增大的基础上,分别于3、7、14、21 d开始持续性调牙合干预,建立I-3d、I-7d、I-14d、I-21d调牙合组,所有大鼠第28天处死,取大鼠一侧TMJ进行苏木精.伊红(Hematoxylin-eosin,HE)染色、免疫组织化学 (Immunohistochemistry,IHC) 染色,另一侧TMJ滑膜组织进行实时定量聚合酶链反应(Real-time polymerase chain reaction ,Real-time PCR)检测TMJ滑膜COX-2和iNOS的表达情况。结果:调牙合组COX-2、iNOS mRNA表达较对照组明显减弱(P<0.05)。I-3d调牙合组几乎不表达(P<0.005)。结论:早期调牙合能减轻大鼠前导增大引起的滑膜炎症程度,同时降低其滑膜炎症因子COX-2、iNOS的表达。

Abstract: Objective: To investigate the effect of occlusal intervention on the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in TMJ synovium of rats caused by increased anterior guidance. Methods: Metal crowns increased 30 degree on palatal side were cemented to the maxillary incisors of rat to build the model of anterior guidance increased group (C). The intervention group was built by increased the anterior guidance of rat first, then the occlusal intervention respectively started on the day 3, 7, 14, and 21 to build the I-3d, I-7d, I-14d, and I-21d intervention group. All rats were sacrificed on day 28. TMJ joints were extracted by one side. HE staining was processed to observe the histological changes of TMJ synovium. IHC and real-time PCR analysis were evaluated to detect the levels of COX-2 and iNOS in TMJ synovium. Results: The mRNA of iNOS and COX-2 barely expressed in the intervention groups (I) compared with the anterior guidance increased group (C) (P<0.05). iNOS and COX-2 were scarcely expressed in the group I-3d. Conclusion: On the basis of increasing the anterior guidance inclination of rat, the early intervention would lead to much less expression of iNOS and COX-2 in TMJ synovium of rat.

Key words: Anterior guidance , Occlusal intervention, Temporomandibular joint synovium, Cyclooxygenase-2Inducible nitric oxide synthase

中图分类号:

R782.6

李雨轩, 姚天华, 白乐康, 谭文宏, 韩会芳, 翟桐, 肖敏. 调牙合对前导增大的大鼠TMJ滑膜中COX-2、iNOS转归变化的研究[J]. 口腔医学研究, 2017, 33(4): 362-366.

LI Yu-xuan, YAO Tian-hua, BAI Le-kang, TAN Wen-hong, HAN Hui-fang, ZHAI Tong, XIAO Min.. Effect of Occlusal Intervention on the Expression of COX-2 and iNOS in the Temporomandibular Joint Synovial Membrane of Rat Caused by Increased Anterior Guidance.[J]. Journal of Oral Science Research, 2017, 33(4): 362-366.

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调牙合对前导增大的大鼠TMJ滑膜中COX-2、iNOS转归变化的研究

Effect of Occlusal Intervention on the Expression of COX-2 and iNOS in the Temporomandibular Joint Synovial Membrane of Rat Caused by Increased Anterior Guidance.

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