口腔医学研究

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (4): 378-381.DOI: 10.13701/j.cnki.kqyxyj.2017.04.008

• 临床研究论著 • 上一篇    下一篇

新疆维吾尔族儿童龋病与HLA-DRB1等位基因多态性的相关性研究

张瑞涵, 刘佳, 郭冉, 刘奕杉*   

  1. 新疆医科大学第一附属医院儿牙预防保健科 新疆 乌鲁木齐 830054
  • 收稿日期:2016-09-23 出版日期:2017-04-20 发布日期:2017-04-24
  • 通讯作者: 刘奕杉,E-mail:lys-tree@126.com
  • 作者简介:张瑞涵(1988~ ),女,四川人,硕士,住院医师,主要从事龋病防治研究工作。
  • 基金资助:
    国家自然科学基金资助项目(编号:81560178)

Association between Dental Caries and HLA-DRB1 Allele Polymorphisms among Uygur Children in Xinjiang.

ZHANG Rui-han, LIU Jia, GUO Ran, LIU Yi-shan*.   

  1. Department of Pediatric Dentistry & Oral Prevention and Health Care, the First Affiliated Hospital of Xinjiang Medical University.Urumqi 830054,China
  • Received:2016-09-23 Online:2017-04-20 Published:2017-04-24

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摘要： 目的:探讨人类白细胞抗原(human leucocyte antigen,HLA) -DRB1等位基因多态性与新疆维吾尔族儿童龋病的相关性。方法:采用聚合酶链式反应-序列特异性引物(polymerase chain reaction-sequence specific primer, PCR-SSP)DNA分型技术对40例新疆维吾尔族高龋儿童和40例新疆维吾尔族健康对照儿童进行HLA-DRB1基因分型,研究HLA-DRB1基因多态性与新疆维吾尔族儿童龋病的相关性。结果:高龋组与对照组在HLA-DRB1座位共检出13个特异性基因位点。两组等位基因频率分布有相同之处,均表现为HLA-DRB1* 01基因表达最高(≥47.5%),HLA-DRB1* 08和*09基因表达最低(≤12.5%);高龋组中HLA-DRB1* 11和*13等位基因频率(分别为40.0%和47.5%)显著高于对照组(分别为17.5%和17.5%),差异均有统计学意义(P＜0.05,OR值分别为3.143和3.619);而HLA-DRB1* 04和*07等位基因频率(分别为15.0%和17.5%)显著低于对照组(分别为37.5%和50.0%),差异均有统计学意义(P＜0.05,OR值分别为0.294和0.212)。结论:新疆维吾尔族儿童龋病与HLA-DRB1等位基因多态性具有一定的相关性;HLA-DRB1* 11和*13等位基因可能是该人群的龋病易感基因,HLA-DRB1* 04和*07等位基因可能是该人群的龋病保护基因。

Abstract: Objective: To investigate the association of human leucocyte antigen (HLA)-DRB1 allele polymorphisms with dental caries among Uygur children in Xinjiang. Methods: A total of 80 Uygur children in Xinjiang were enrolled for this study. Among them,40 children were diagnosed as high dental caries and the other 40 children were healthy controls. All 80 children were tested by polymerase chain reaction sequence specific primers (PCR - SSP) DNA parting technology to study the HLA-DRB1 allele polymorphism and dental caries. Results: Thirteen specific loci were detected in HLA-DRB1 locus in high caries group and control group.Two groups of allele frequencies distribution were similar. The expression of HLA-DRB1 *01 gene was the highest (≥47.5%), while expression of HLA-DRB1* 08 and *09 genes was the lowest (≤12.5%).The frequencies of HLA-DRB1*11 and DRB1*13 alleles in the high caries group (40.0% and 47.5%) were significantly higher than those in the control group (17.5% and 17.5%) (P<0.05, OR =3.143 and 3.619). The frequencies of HLA-DRB1* 04 and DRB1*07 alleles in the high caries group (15.0% and 17.5%) were significantly lower than those in the control group (37.5% and 50.0%) (P<0.05, OR =0.294 and 0.294). Conclusion: The susceptibility to caries among Uygur children in Xinjiang is related to the HLA-DRB1 allele. The HLA-DRB1* 11 and *13 might be the susceptible alleles for caries, and the HLA-DRB1* 04 and *07 might be the protected alleles for caries in this population.

Key words: Dental caries, Human leucocyte antigen, Genetic polymorphism , Uygur children

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