口腔医学研究

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (9): 920-923.DOI: 10.13701/j.cnki.kqyxyj.2017.09.003

• 基础研究论著 • 上一篇    下一篇

花粉与P123双模板制备的新型多级孔生物活性玻璃对MG63细胞增殖和分化的影响

汪洋1，申玉芹2，凌新连1，刘宇妍1，秦甜甜1，刁梦雪3，孙新华1*   

  1. 1. 吉林大学口腔医学院正畸科,吉林省牙发育及颌骨重塑与再生重点实验室 吉林 长春 130021；
    2. 吉林大学口腔医学院牙周病科 吉林 长春 130021；
    3. 吉林大学食品科学与工程学院 吉林 长春 130000
  • 收稿日期:2017-02-02 出版日期:2017-09-20 发布日期:2017-09-27

Effect of New Type Multistage Aperture Bioactive Glasses Prepared by Pollen and P123 Dual Template on Proliferation and Differentiation of MG63 Cells

WANG Yang1,SHEN Yu-qin2,LING Xin-lian1,LIU Yu-yan1,QIN Tian-tian1,DIAO Meng-xue3,SUN Xin-hua1*   

  1. 1. Department of Orthodontics, School and Hospital of Stomatology, Jilin University, Changchun 130021, China;
    2. Department of Periodontology, School and Hospital of Stomatology, Jilin University, Changchun 130021, China;
    3. College of Food Science and Engineering, Jilin University, Changchun 130000, China.
  • Received:2017-02-02 Online:2017-09-20 Published:2017-09-27

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摘要： 目的:检测花粉与P123双模板制备的新型多级孔生物活性玻璃对人前成骨细胞系MG63增殖及成骨分化的影响。方法:在材料工作浓度为0.2 g/mL的浸提液(含10%胎牛血清的DMEM)中,培养MG63细胞至1、3、5 d,分别采用MTT比色法检测细胞增殖水平;碱性磷酸酶试剂盒检测细胞内ALP活性;实时定量PCR法测定成骨标志性基因RUNX-2、SP7、COL-1的mRNA表达水平。结果:花粉与P123双模板制备的新型多级孔生物活性玻璃可促进MG63细胞增殖,增强ALP活性表达,上调MG63标志性基因RUNX-2、SP7、COL-1的mRNA表达水平。结论:以花粉与P123双模板制备的新型多级孔生物活性玻璃具有细胞毒性低,且具有促进成骨细胞成骨向分化的潜能。

关键词: 新型多级孔生物活性玻璃, MG63细胞, 增殖, 分化

Abstract: Objective: To investigate the effects of new type multistage aperture bioactive glasses which were prepared by pollen and P123 dual template on the proliferation and differentiation of MG63 cells. Methods: MG63 cells were cultured in the material leach liquor (0.2g/ml working concentration, DMEM with 10% fetal bovine serum) for 1, 3, and 5 days. MTT colorimetric method was used to detect the level of cell proliferation. Alkaline phosphatase kits was used to measure the ALP activity. Real-time PCR was used to analyze the mRNA expression of osteogenesis marker, i.e. RUNX-2, SP7, and COL-1. Results: The material promoted the proliferation of MG63 cells, enhanced the activity of ALP, up-regulated the mRNA expression level of markers gene RUNX-2, SP7, and COL-1. Conclusion: The new type multistage aperture bioactive glasses which were prepared by pollen and P123 dual template has low cytotoxicity and the potential to promote the osteogenetic differentiation.

Key words: Multistage aperture bioactive glasses, MG63 , Proliferation , Differentiation

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