口腔医学研究

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (9): 916-919.DOI: 10.13701/j.cnki.kqyxyj.2017.09.002

• 基础研究论著 • 上一篇    下一篇

红景天苷对成骨细胞的作用及相关分子机制研究

梅国华1，郭小芹2，陈京京2，王越2*，李正阳3*   

  1. 1. 南开大学医学院 天津 300071；
    2. 武警后勤学院病原生物学与免疫学教研室 天津 300309；
    3. 天津市口腔医院正畸科 天津 300041
  • 收稿日期:2017-03-26 出版日期:2017-09-20 发布日期:2017-09-27
  • 通讯作者: 王越,E-mail: wangyue6808@126.com 李正阳,E-mail:lizhengyang2006@hotmail.com
  • 作者简介:梅国华(1987~ ),男,河北人,硕士在读,主要从事口腔正畸方面研究。
  • 基金资助:
    国家自然科学基金项目(编号:81273520、81502256、81572852)国家临床重点专科建设项目(口腔正畸专业)批准文号:国卫办医函[2013]544号天津市口腔医院博硕士重点项目(编号:2015BSZD13)

Effect of Rhodiola Rosea Glycosides on Function of Osteoblasts and Related Molecular Mechanism

MEI Guo-hua1, GUO Xiao-qin2, CHEN Jing-jing2, WANG Yue2*, LI Zheng-yang3*   

  1. 1. Medical College of Nankai University, Tianjin 300071, China;
    2. Department of Pathogen Biology and Immunology of Chinese People's Armed Police Forces, Tianjin 300309, China;
    3. Tianjin Stomatology Hospital, Tianjin 300041, China.
  • Received:2017-03-26 Online:2017-09-20 Published:2017-09-27

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摘要： 目的:研究红景天苷(SAL)对人成骨样MG-63细胞增殖、分化和HIF-1α依赖的VEGF表达的影响及相关分子机制。方法:采用MTS法、流式细胞术分析、RT-PCR、ELISA、Western blot等技术观察SAL对MG-63细胞增殖、分化和HIF-1α依赖的VEGF表达的影响,进一步探讨其相关分子机制。结果:SAL通过调控MG-63细胞的周期分布及上调Runx2和Osterix表达促进成骨细胞的增殖和分化。另外,SAL可明显上调下游靶基因VEGF的表达。结论:SAL可显著促进成骨样MG-63细胞的增殖、分化以及HIF-1α依赖的VEGF的表达,进而促进骨和血管新生。

关键词: 红景天苷, 成骨细胞, 低氧诱导因子, 血管内皮生长因子

Abstract: Objective: To study the effects of salidroside (SAL) on proliferation, differentiation, mineralization, and the expression of VEGF depending on HIF-1α of MG-63 cells and related molecular mechanisms. Methods: To study the effects SAL on proliferation, differentiation, mineralization, the expression of VEGF depending on HIF-1α, and related molecular mechanisms, the cell vitality assay, Flow cytometry analysis, semi-quantitative reverse transcription polymerase chain reaction, ELISA and Western blot were used. Results: SAL promoted the proliferation and differentiation of MG-63 cells through regulating the distribution of cell cycle, the mRNA and protein levels and the expression of HIF-1α. SAL could also increase the VEGF production of downstream target genes of MG-63 cells. Conclusion: In vitro experiment study shows that SAL can promote proliferation, differentiation, mineralization, the production of HIF-1α and downstream target genes VEGF of MG-63 cells to promote the formation of new bones and vessels.

Key words: Salidroside (SAL) , Osteoblast, Hypoxia inducible factor-1α (HIF-1α) , Vascular endothelial , growth factor (VEGF)

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