口腔医学研究

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (10): 1023-1026.DOI: 10.13701/j.cnki.kqyxyj.2017.10.001

• 名家论道 •    下一篇

自组装纳米粒子防龋疫苗的免疫效能

叶倩琳1，李祯2#，曹茜茜1，刘畅1*，樊明文1*   

  1. 1. 口腔基础医学省部共建国家重点实验室培育基地和口腔生物医学教育部重点实验室,武汉大学口腔医学院 湖北 武汉 430079；
    2. 武汉大学中南医院麻醉科 湖北 武汉 430071
  • 收稿日期:2017-08-16 出版日期:2017-10-20 发布日期:2017-10-24
  • 通讯作者: 刘畅,E-mail:liuc0728@whu.edu.cn,樊明文,E-mail:fmwmail@whu.edu.cn
  • 作者简介:叶倩琳(1992～ ),女,山东人,硕士,主要从事牙体牙髓病学基础与临床研究工作。李祯(1987~ ),男,湖南人,博士,主要从事镇痛药物与骨质疏松及骨免疫相关基础与临床研究工作。
  • 基金资助:
    国家自然科学基金项目(编号:210900019)

Study on Immune Efficacy of Self-assembling Anti-caries Nanoparticle Vaccine

YE Qian-lin1, LI ZHEN2, CAO Xi-xi1, LIU Chang1, FAN Ming-wen2*   

  1. 1.The State Key Laboratory Breeding of Basic Science of Stomatology (Hubei- Most) & Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China;
    2.Department of Anesthesiology, Zhongnan Hospital, Wuhan University, Wuhan 430071, China.
  • Received:2017-08-16 Online:2017-10-20 Published:2017-10-24

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摘要： 目的:研究自组装纳米粒子防龋疫苗Glu-FTH以及Glu + Poly(I:C)(联合应用佐剂Poly(I:C)与抗原Glu)对小鼠特异性体液免疫和黏膜免疫的影响。方法:将小鼠随机分为6组,分别将Glu-FTH、Glu、Glu-FTH+Poly(I:C)、Glu+Poly(I:C)、FTH、PBS经鼻黏膜滴注免疫小鼠,酶联免疫吸附试验(ELISA)检测血清和唾液中特异性抗体水平。结果:Glu-FTH、Glu、Glu-FTH+Poly(I:C)、Glu+Poly(I:C)均可有效提高小鼠血清中抗Glu IgG水平;Glu+Poly(I:C)和Glu可有效提高小鼠唾液中抗Glu sIgA水平。结论:防龋疫苗Glu-FTH具有一定的免疫效果,佐剂Poly(I:C)与抗原Glu联合应用可引发较强的免疫反应。

关键词: 龋齿, 自组装纳米粒子疫苗, Poly(I:C), 佐剂, 黏膜免疫

Abstract: Objective: To study the efficacy of self-assembling anti-caries nanoparticle vaccine Glu-FTH and Glu + Poly (I:C) on inducing protective humoral immune response and mucosal immune response in mice. Methods: The mice were separated into 6 groups randomly. Glu-FTH, Glu, Glu-FTH+Poly (I:C), Glu+Poly (I:C), FTH and PBS were respectively delivered into mice by intranasal administration. The levels of specific antibodies in serum and saliva were tested by enzyme-linked immunosorbent assay (ELISA). Results: The levels of serum anti-Glu IgG in Glu-FTH, Glu, Glu-FTH+Poly (I:C), Glu+Poly(I:C) group increased. The levels of salivary anti-Glu sIgA in Glu+Poly(I:C) and Glu also increased. Conclusion: Anti-caries vaccine Glu-FTH and the synergy of Poly (I:C) and Glu can induce specific immune response.

Key words: Caries, Self-assembling nanoparticle vaccine, Poly(I:C), Adjuvant, Mucosal immune

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