口腔医学研究

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (12): 1265-1268.DOI: 10.13701/j.cnki.kqyxyj.2016.12.010

• 临床研究论著 • 上一篇    下一篇

龋齿及牙周病患者与健康人群口腔微生态的比较研究

史彦1,李成杰2,张明正2,王鑫3,杨少国3,孟凡景3,陈廷涛3*,杨健1*   

  1. 1. 南昌大学附属口腔医院牙体牙髓科 江西 南昌 330006;
    2. 南昌大学第二临床医学院 江西 南昌 330006;
    3. 南昌大学转化医学研究院 江西 南昌 330031
  • 收稿日期:2016-08-03 出版日期:2016-12-25 发布日期:2016-12-26
  • 通讯作者: 陈廷涛,E-mail:chentingtao1984@163.com
    杨健,E-mail:jianyang399@hotmail.com
  • 作者简介:史彦(1976~ ),女,江西南昌人,硕士,副主任医师,副教授,研究方向为牙体牙髓病学。
  • 基金资助:
    国家自然科学基金地区基金项目(编号:81503364/31560264)
    江西省科技厅项目(编号:20151BBG70239)

Comparison of Oral Microbial Diversity in Healthy People and Patients with Dental Caries and Periodontal Disease.

SHI Yan1, LI Chen-jie2, ZHANG Ming-zheng2, WANG Xin3, YANG Shao-Guo3, MENG Fan-Jin3, CHEN Ting-Tao3*, YANG Jian1*.   

  1. 1. Department of Endodontics,Stomatology Hospital of Nanchang University,Nanchang 330006,China;
    2. The Second Clinical Medical College, Nanchang University,Nanchang 330006,China;
    3. Institute of Translational Medicine, Nanchang University, Nanchang 330031, China.
  • Received:2016-08-03 Online:2016-12-25 Published:2016-12-26

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摘要： 目的:利用高通量测序技术比较健康人和口腔患者口腔微生物的组成和变化。方法:选取典型的龋齿和牙周病患者,收集受刺激的唾液和口咽样本并进行高通量测序,利用生物信息学分析和比较各样品之间的物种组成差异。结果:各组的平均操作分类单元(OTU)为315个,其中健康人口腔内硬壁菌(Firmicutes)、拟杆菌(Bacteroidetes)、蛋白菌(Proteobacteria)、放线杆菌(Actinobacteria)和梭杆菌(Fusobacteria)对应的OTU数目分别73、64、53、19和18个;此外,相对丰富度结果表明硬壁菌(Firmicutes)在儿童组中减少而在青年组和成年组中增多,而蛋白菌(Proteobacteria)在儿童组中增多而在青年组和成年组中减少。结论:年龄段和口腔疾病对牙齿的发育和口腔微生物的组成影响巨大,传统意义上的致病菌可能在口腔健康中同样发挥着维护口腔稳态的作用。

关键词: 高通量测序, 龋齿, 牙周炎, 微生物多样性

Abstract: Objective: To compare the microbial diversity of healthy people and oral patients with high-throughput sequencing method and to provide basic data for curing dental caries and periodontal diseases. Methods: The patients with typical dental caries and periodontal diseases were selected. Their stimulated whole saliva and oropharyngeal samples were collected for high-throughput sequencing analysis and the microbial diversity among samples were analyzed using bioinformatics. Results: The Venn results indicated that a mean of 315 operational taxonomic units (OTUs) was obtained, and 73, 64,53, 19 and 18 common OTUs belonging to Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria and Fusobacteria were identified in healthy people. In addition, the relative richness results indicated that the Firmicute was reduced in children group and increased in youth group and adult group, while the Proteobacteria was increased in children group but reduced in youth group and adult group. Conclusion: The age bracket and oral disease had largely influenced the tooth development and microbial development in oral cavity, and the traditional "pathogenic bacteria" of Firmicutes, Proteobacteriaand Bacteroidetes might exert beneficial effects on oral health.

Key words: High-throughput sequencing analyses, Dental caries, Periodontal disease, Microbial diversity