口腔医学研究

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (11): 1169-1172.DOI: 10.13701/j.cnki.kqyxyj.2017.11.009

• 牙周组织研究 • 上一篇    下一篇

唑来膦酸对牙龈成纤维细胞生物学特性的影响研究

董文杰1, 王禹錕1, 岳文江2*   

  1. 1. 牡丹江医学院第二附属医院口腔科 黑龙江 牡丹江 157000;
    2. 牡丹江医学院第二附属医院五官科 黑龙江 牡丹江 157000
  • 收稿日期:2017-06-19 出版日期:2017-11-20 发布日期:2017-11-29
  • 通讯作者: 岳文江,电话:13206887799
  • 作者简介:董文杰(1975~ ),女,河北省苏路县人,学士,主治医师,主要从事口腔医学方面的工作。
  • 基金资助:
    黑龙江省自然科学基金项目(编号:H201596)

Effects of Zoledronic Acid on Biological Characteristics of Gingival Fibroblasts

DONG Wen-jie1, WANG Yu-kun1, YUE Wen-jiang2*   

  1. 1. Department of Stomatology, the Second Affiliated Hospital of Mudanjiang Medical University, Mudanjiang 157000, China;
    2. Department of Ophthalmology and Otorhinolaryngology, the Second Affiliated Hospital of Mudanjiang Medical University, Mudanjiang 157000, China
  • Received:2017-06-19 Online:2017-11-20 Published:2017-11-29

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摘要： 目的:探讨唑来膦酸对牙龈成纤维细胞增殖、凋亡、分化的影响及机制。方法:从牙龈组织分离原代人牙龈成纤维细胞(HGFs),0.5、1、5、10 μmol/L的唑来膦酸处理HGFs 24、48、72 h后,CCK8实验检测细胞的增殖;流式细胞仪检测10 μmol/L的唑来膦酸处理HGFs 72 h后的细胞凋亡情况;加入10 μmol/L的唑来膦酸的成骨诱导分化液培养HGFs 7 d后,碱性磷酸酶(ALP)试剂盒检测ALP活性,Western blot检测Runx2和OPN的蛋白表达;10 μmol/L的唑来膦酸处理HGFs 72 h后,Western blot检测NF-κB信号通路p65、IκBα蛋白表达。结果:唑来膦酸可呈剂量依赖抑制HGFs细胞增殖(P<0.01);唑来膦酸组细胞的凋亡率显著高于对照组,ALP活性及Runx2、OPN蛋白表达显著高于对照组,p65、IκBα的蛋白表达显著低于对照组(P<0.01)。结论:唑来膦酸可抑制HGFs增殖,促进细胞的凋亡和分化,其机制与下调NF-κB信号通路有关。

关键词: 唑来膦酸, 牙龈成纤维细胞, 增殖, 凋亡, 分化, NF-κB信号通路

Abstract: Objective: To investigate the effect and mechanism of zoledronic acid on proliferation, apoptosis, and differentiation of gingival fibroblasts. Methods: Primary human gingival fibroblasts (HGFs) isolated from the gingival tissue were treated with 0.5, 1, 5, and 10 μmol/L zoledronic acid for 24, 48, and 72h. Cell proliferation was detected by CCK8 assay. Cell apoptosis was detected by flow cytometry in 10 μmol/L zoledronic acid treated HGFs cells for 72h. Alkaline phosphatase (ALP) activity was detected by ALP kit, the expression of Runx2 and OPN protein were detected by Western blot as well as p65 and IκBα protein after treated with 10 μmol/L zoledronic acid for 72h. Results: Zoledronic acid inhibited proliferation of HGFs in a dose dependent manner (P<0.01). Apoptosis rate of cells in zoledronic acid was significantly higher than that of control group. ALP activity and the expression of Runx2 and OPN protein were significantly higher than those of control group, whereas the protein expressions of p65 and IκBα were significantly lower than the control group (P<0.01). Conclusion: Zoledronic acid can inhibit the proliferation but promote the apoptosis and differentiation of HGFs by downregulating NF-κB signaling pathway.

Key words: Zoledronic acid, Gingival fibroblast, Proliferation, Apoptosis, Differentiation, NF-κB pathway

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