口腔医学研究

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (4): 428-431.DOI: 10.13701/j.cnki.kqyxyj.2018.04.021

• 口腔材料学研究 • 上一篇    下一篇

纳米氢氧化钙制备方法的改良及作为根管治疗糊剂的生物安全性初探

王籽明, 彭问安, 孙青, 范伟*   

  1. 武汉大学口腔医学院 湖北 武汉 430079
  • 收稿日期:2017-12-20 出版日期:2018-04-28 发布日期:2018-04-25
  • 通讯作者: 范伟,E-mail:fwxj001@163.com
  • 作者简介:王籽明(1996~ ),女,湖北人,学士,主要从事根管 治疗充填材料的研制。
  • 基金资助:
    国家大学生创新创业基金(编号:201610486111)

Modification of Preparation Method of Nanometer Calcium Hydroxide and Preliminary Assessment of Biological Safety for Root Canal Therapy.

WANG Zi-ming, PENG Wen-an, SUN Qing, FAN Wei*   

  1. School of Stomatology, Wuhan University, Wuhan 430079, China.
  • Received:2017-12-20 Online:2018-04-28 Published:2018-04-25

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被引次数

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摘要： 目的:改良纳米氢氧化钙的制备方法并对其作为根管治疗糊剂的生物安全性进行初步评价。方法:以CaCl2和NaOH为沉淀反应物,改良环节包括纳米氢氧化钙的制备系统,改性剂用聚乙二醇,加快反应速度为超声波振动,干燥分散纳米粉体为正丁醇共沸蒸馏干燥法。实验样品制得后分别选用红外线光谱仪、透射电子显微镜检测其组成成分和粒径大小,再将样品与医用碘仿和甘油调制成符合临床根管治疗要求糊剂,用扫描电子显微镜检测其形貌。后将实验分为阴性、阳性对照组和纳米氢氧化钙糊剂组,观察各组红细胞溶血反应及溶血率。结果:改良后制备装置自动化程度更高,操作更准确、安全, 能防尘、隔菌,显著缩短制备时间,节省人力和能源,降低成本,制备的粉体颗粒更分散、均匀,不发生软硬团聚。样品由聚乙二醇和氢氧化钙组成,粒径大约在100~200 nm之间,呈球形和链球形。作为根管治疗糊剂,其表面致密、光滑,横截面积大、呈六方体结构,颗粒间隙小,排列紧凑、致密;与阳性对照组比较,纳米氢氧化钙糊剂组不引起溶血反应,其血红蛋白相对值明显低于阳性对照组(P<0.01),溶血率为4.54%,显著小于医用植入材料要求红细胞溶血率≤5。结论:本实验改良方法科学合理,其表征为纳米级、分散均匀而无团聚的氢氧化钙,作为根管治疗糊剂初步证实具有生物安全性,值得在临床上推广和开发、具有广泛发展前途。

关键词: 纳米氢氧化钙及其糊剂, 制备方法改良, 表征, 生物安全性

Abstract: Objective: To modify the preparation method of nanometer calcium hydroxide (nano-Ca(OH)2) and assess the preliminarily biological safety for root canal therapy. Methods: Nano-Ca(OH)2 was prepared by use of synthetic nano-precipitation titration of Ca(OH)2 materials with the raw materials for CaCl2 and NaOH as well as polyethylene glycol (PEG). According to modified method, the whole procedure was as following: PEG parceled first CaCl2 and reacted with NaOH, and ultrasonic wave was used to vibrate reaction so that to accelerate production of nano-Ca(OH)2 and prevent agglomeration of particles, as well as drying method of heterogeneous azeotropic distillation was made to divergence the nano-Ca(OH)2 gel. The infrared spectroscopy and transmission electron microscopy were used to detecte the physical and chemical characteristics of nano-Ca(OH)2. Nano-Ca(OH)2 paste that was mixed with medical iodoform and glycerol was used as root canal therapy. The scanning electron microscopy was used to observe the shape of specimens. The experiments were divided into negative group, positive group, and Nano-Ca(OH)2 paste group. Results: The modified treatment had higher automaticity, could isolate bacterial and dust particle, and decrease manufacture time, work intensity, and cost. The nano-powder distributed much more evenly without agglomeration. The nano-article of Ca(OH)2 was about 100-200nm, in the round and hammer shape. As nano-Ca(OH)2 paste, it had dense structure, compact arrangement, and smooth surface, which was better than ordinary Ca(OH)2 paste. Furthermore, nano-Ca(OH)2 paste could not interact with hemoglobin and maintain the morphological integrity and smooth surface of red blood cells. Hemoglobin value was sign-ificantly decreased in nano-Ca(OH)2 paste group compared with positive group (P<0.01). Conclusion: The modified manufacture method was scientific and reasonable. Nano-level and uniform Ca(OH)2 was produced, which could prevent the hemolytic of humanred blood cells and be used as paste for root canal therapy.

Key words: Nanometer calcium hydroxide, Modification of manufacturing method, Physical characterization, Biological safety