口腔医学研究

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (11): 1253-1256.DOI: 10.13701/j.cnki.kqyxyj.2018.11.025

• 颞下颌关节研究 • 上一篇    下一篇

沉默信息调节因子1在颞下颌关节骨关节炎滑膜中表达的相关研究

刘丽1, 冯亚平1#, 赵苏立2, 龙星1, 柯金1*   

  1. 1. 武汉大学口腔医院口腔颌面外科 湖北 武汉 430079;
    2. 宜昌市第一人民医院口腔科 湖北 宜昌 443000
  • 收稿日期:2018-09-17 出版日期:2018-11-28 发布日期:2018-11-23
  • 通讯作者: 柯金,E-mail:kejin@whu.edu.cn
    #为共同第一作者
  • 作者简介:刘丽(1996~ ),女,山东人,硕士在读,主要从事颞下颌关节相关的基础研究工作。冯亚平(1987~ ),女,山东人,医师,博士,主要从事颞下颌关节相关的临床及基础研究。
  • 基金资助:
    国家自然科学基金(编号:81870789)

Expression of SIRT1 in Synovium of Temporomandibular Joint Osteoarthritis

LIU Li1, FENG Ya-ping1#, ZHAO Su-li2, LONG Xing1 , KE Jin1*   

  1. 1. Department of Oral and Maxillofacial Surgery, Stomatology Hospital of Wuhan University, Wuhan 430079, China;
    2. Department of Stomatology, Yichang First People's Hospital, Yichang 443000, China.
  • Received:2018-09-17 Online:2018-11-28 Published:2018-11-23

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摘要： 目的:探讨沉默信息调节因子1(silent information regulator 1,SIRT1)在颞下颌关节骨关节炎(temporomandibular joint osteoarthritis,TMJ OA)滑膜组织细胞中表达的差异性。方法:苏木素-伊红染色观察滑膜组织形态,免疫组织化学染色法检测滑膜组织SIRT1表达及分布情况;滑膜细胞体外培养传代,Western blot及实时定量聚合酶链反应(real-time quantitative polymerase chain reaction,Real-time PCR)法检测滑膜细胞中SIRT1蛋白和基因表达。结果:苏木素-伊红染色结果显示TMJ OA组滑膜较正常组明显增厚,血管增多;免疫组织化学染色法结果显示SIRT1在TMJ OA组染色强度较正常组明显升高(P<0.05);Real-time PCR及Western blot结果显示TMJ OA组滑膜细胞中SIRT1表达明显高于正常组(P<0.05)。结论: SIRT1在TMJ OA滑膜中表达升高,与骨关节炎的发生及发展密切相关,为该病发生的分子机制以及治疗提供一个新的理论依据。

关键词: 颞下颌关节骨关节炎, 滑膜组织, 滑膜细胞, 沉默信息调节因子1

Abstract: Objective: To investigate the expression of SIRT1 in the synovial of temporomandibular joint osteoarthritis(TMJ OA). Methods: HE staining was used to observe the synovial tissue morphology. Immunohistochemistry was used to detect the expression and distribution of SIRT1 in synovial tissue. The human TMJ synovial cells were cultured in vitro by tissue explantation. Western blotting and real-time PCR were used to detect the SIRT1 protein and gene expression in synovial cells. Results: HE staining showed that the synovial membrane of the OA group was significantly thicker than the normal group, and the blood vessels increased. Immunohistochemistry showed that the staining intensity of SIRT1 in the OA group was significantly higher than that in the normal group (P<0.05). Real-time PCR and Western blotting showed that the expression of SIRT1 in synovial cells of OA group was significantly higher than that of normal group (P<0.05). Conclusion: The expression of SIRT1 in the synovium of TMJ OA is closely related to the occurrence and development of osteoarthritis, providing a new theoretical basis for the molecular mechanism and treatment of this disease.

Key words: Temporomandibular joint osteoarthritis, Synovial tissue, Synovial cells, Silent information regulator 1