人前磨牙颈部牙釉质和牙骨质表面形貌的观察

doi:10.13701/j.cnki.kqyxyj.2019.10.019

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (10): 989-993.DOI: 10.13701/j.cnki.kqyxyj.2019.10.019

人前磨牙颈部牙釉质和牙骨质表面形貌的观察

孔宁华¹, 施生根², 吕亚林¹, 刘林^1*

1. 首都医科大学附属北京安贞医院口腔科北京 100029;
2. 战略支援部队特色医学中心口腔科(原解放军第306医院) 北京 100101

收稿日期:2019-01-17 出版日期:2019-10-28 发布日期:2019-10-22
通讯作者: 刘林,E-mail:handongnm@sina.cn
作者简介:孔宁华(1982~ ),男,山东曲阜人,博士,主治医师,研究方向:牙齿表面、种植体表面形貌研究。

Morphological Analysis of Dental Enamel and Root Cementum at the Cervical Region

KONG Ninghua¹, SHI Shenggen², LV Yalin¹, LIU Lin^1*

1. Department of Stomatology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China;
2. Department of Stomatology,Strategic Support Force Characteristic Medical Center(306 Hospital of PLA), Beijing 100101, China

Received:2019-01-17 Online:2019-10-28 Published:2019-10-22

摘要/Abstract

摘要： 目的:采用场发射环境扫描电子显微镜和原子力显微镜观察人前磨牙颊面颈部穿龈区牙釉质和牙骨质表面形貌。方法:选择因正畸治疗需要拔除的人第一恒前磨牙为实验牙,以颊面釉牙骨质界顶点为基准,分别向冠方、根方、近中、远中各延伸1 mm,获取厚约2 mm,面积约2 mm×2 mm的样品。经筛选后,牙釉质及牙骨质分别随机选择5个样品用于场发射环境扫描电子显微镜观察,20个样品用于原子力显微镜观察。测量牙釉质和牙骨质表面轮廓算术平均粗糙度(Ra)和轮廓均方根粗糙度(Rq),并统计分析结果。结果:人前磨牙颊面颈部穿龈区牙釉质表面均以波浪状的叠瓦形貌为特征,自颈部开始,釉质一层一层叠加,叠瓦层边缘钝圆而呈阶梯状排列,边缘不规则。叠瓦形貌高起处相对光滑,隐约可见浅凹;低凹处表面粗糙,多凹陷。凹陷的外形基本保持六角形,直径约4~6 μm。牙骨质表面较均匀,呈现无数突起,均以乳突状形貌或丘形形貌为主。牙釉质表面Ra及Rq的平均值分别为(0.459±0.223) μm和(0.580±0.248) μm,牙骨质表面Ra及Rq的平均值分别为(0.685±0.229) μm和(0.796±0.261) μm,牙釉质表面Ra及Rq分别小于牙骨质表面Ra及Rq,差异均有统计学意义(P<0.05)。结论:人前磨牙颊面颈部穿龈区牙釉质表面以横纹状形貌和凹陷状形貌为主,牙骨质表面均以乳突状形貌或丘形形貌为主,牙釉质表面较牙骨质表面光滑。

关键词: 牙釉质, 牙骨质, 表面形貌

Abstract: Objective: To characterize dental enamel and root cementum at the cervical region of healthy human teeth by using field-emission scanning electron microscopy (FESEM) and atomic force microscopy (AFM). Methods: Sound human first premolars freshly extracted for orthodontic reasons were included in the study. The enamel surface on the buccal side of the tooth was analyzed in a narrow area, 1mm coronal to the zenith of cemento-enamel junction (CEJ). The cementum surface on the buccal side of the tooth was analyzed in a narrow area, 1mm apical to the zenith of CEJ. The value of Ra and Rq was measured. Results: The gross appearance of the cervical enamel was characterized by overlapping wave-like layers. The border of each enamel layer was highly undulated and formed in most areas a distinct rounded step towards the underlying layer. The exposed surface of each enamel layer was relatively smooth with very shallow depressions of Tomes' processes pits. The diameter of the Tomes' process pit was 4 to 6 microns. The cementum was characterized by projections above the general plane of the mineralizing front. The mean Ra and standard deviation in μm were: enamel, 0.459±0.223; cementum, 0.685±0.229. The mean Rq and standard deviation in μm were: enamel, 0.580±0.248; cementum, 0.796±0.261. There were statistically significant differences (P<0.05) among Ra and Rq between enamel and root cementum. Conclusion: Dental enamel surface and root cementum surface at the cervical region showed a clear difference in topography. Enamel surfaces were slightly smoother than root cementum surfaces. The findings from the study of the morphology of teeth gave us va-luable insights for designing percutaneous implants.

Key words: enamel, cementum, morphology

孔宁华, 施生根, 吕亚林, 刘林. 人前磨牙颈部牙釉质和牙骨质表面形貌的观察[J]. 口腔医学研究, 2019, 35(10): 989-993.

KONG Ninghua, SHI Shenggen, LV Yalin, LIU Lin. Morphological Analysis of Dental Enamel and Root Cementum at the Cervical Region[J]. Journal of Oral Science Research, 2019, 35(10): 989-993.

参考文献

[1] Myshin HL, Wiens JP. Factors affecting soft tissue around dental implants: A review of the literature [J]. J Prosthet Dent, 2005, 94(5):440-444.
[2] 王婧,顾新华.种植体基台表面性状及其对软组织附着的影响[J].口腔医学,2017,37(10):942-945.
[3] 黄楠,李敬安,魏来,等.心血管介入器械表面修饰新策略:血管内皮细胞生长微环境的原位仿生构建[J].西南交通大学学报,2016,51(3):571-584.
[4] Gargiulo AW, Wentz F, Orban B. Dimensions and relations of the dentogingival junction in humans [J]. J Periodontol,1961, 32(3):261-267.
[5] Zavala-Alonso V, Martinez-Castanon GA, Patino-Marin N, et al. Characterization of healthy and fluorotic enamel by atomic force microscopy [J]. Microsc Microanal, 2010,16(5):531-536.
[6] 于静涛,王贤辐,刘瑞德,等.牙萌出前后釉质表面结构及显微分析研究[J].中国医科大学学报,1997,26(2):213-214.
[7] Fejerskov O, Josenphsen K, Nyvad B. Surface ultrastructure of unerupted mature human enamel [J]. Caries Res, 1984, 18(4):302-314.
[8] 苏倩倩,李铁军,陈新明.未萌牙釉质表面结构的扫锚电镜观察[J].口腔医学纵横,1988,4(2):89-91.
[9] 高学军,罗桂云,李宏毅. 牙萌出前后釉质表面的扫描电镜观察[J].口腔医学纵横,1988,4(2):92-93.
[10] 吕若静,李玉晶,姚培. 初萌年轻恒牙釉质组织结构的特点[J].北京口腔医学,2003,11(3):125-127.
[11] 张蕴惠,张晋英,戴霞飞. 牙骨质表面性状的超微结构特征及其临床意义[J].华西口腔医学杂志,1990,8(3):158-160.
[12] 朱小玲,孟焕新.快速进展性牙周炎牙骨质表面形貌观察及微区成分分析[J]. 中华口腔医学杂志,2003,38(2):126-128.
[13] Jones SJ. The root surface: an illustrated review of some scanning electron microscope studies [J]. Scanning Microsc, 1987, 1(4):2003-2018.
[14] Vitkov L, Kastner M, Kienberger F, et al. Corrections between AFM and SEM imaging of acid etched tooth enamel [J]. Ultrastructural Path, 2009, 32(1):1-4.
[15] Boyde A. Scanning electron microscopy of enamel surfaces [J]. Br Med Bull, 1975, 31(2):120-124.
[16] Carrassi A, Abati S, Santarelli G.The role of scanning electron microscopy in periodontal research [J]. Scanning Micro, 1988, 2(2):1123-1138.
[17] Edblad T, Hoffman M, Hakeberg M, et al. Micro-topography of dental enamel and root cementum [J]. Swed Dent J, 2009,33(1):41-48.
[18] 陈江,周麟.种植体颈部设计的研究现状[J].口腔疾病防治,2016,24(8):441-444.

人前磨牙颈部牙釉质和牙骨质表面形貌的观察

Morphological Analysis of Dental Enamel and Root Cementum at the Cervical Region

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	苏伟珠, 王贻宁, 马霄. 两种漂白技术对牙釉质、牙本质及釉牙本质界颜色变化及反弹的影响[J]. 口腔医学研究, 2020, 36(8): 786-792.
[2]	金小婷, 史诗, 陈欢欢, 薛冬, 麻健丰. 釉质表面超疏水凝胶纳米涂层的制备及其性能研究[J]. 口腔医学研究, 2020, 36(6): 585-590.
[3]	刘继涛, 陈庆华. 牙釉质的结构和力学性能研究进展[J]. 口腔医学研究, 2020, 36(3): 213-215.
[4]	江瑶, 黎红. 钛表面粗糙度对成纤维细胞生长影响的研究进展[J]. 口腔医学研究, 2019, 35(8): 738-740.
[5]	胡蝶,张凌琳. 口腔来源蛋白及多肽诱导牙釉质仿生矿化的研究进展[J]. 口腔医学研究, 2019, 35(6): 517-520.
[6]	王志平, 赵蒙蒙, 董志恒, 牛月月, 王倩, 王青山. 纳米羟基磷灰石复合树脂与牙釉质之间粘接界面电镜观察[J]. 口腔医学研究, 2019, 35(5): 457-460.
[7]	刘晓默, 张梦琦, 林久祥. 两种托槽正畸治疗前后表面形貌及元素成分变化的研究[J]. 口腔医学研究, 2019, 35(4): 340-345.
[8]	魏秋菊, 李冰. 牙骨质环推断年龄的研究进展[J]. 口腔医学研究, 2019, 35(3): 215-217.
[9]	罗文琼, 苗磊, 潘亚萍. 牙周内窥镜辅助与传统刮治后根面差异的体外研究分析[J]. 口腔医学研究, 2019, 35(12): 1182-1186.
[10]	蔡彦坤, 郑国莹, 隋磊. 纯钛或钛合金种植材料表面不同纳米结构对细胞行为的影响[J]. 口腔医学研究, 2018, 34(7): 699-702.
[11]	孔晶晶, 梁向阳, 李春年, 代鑫鹏, 马向涛. 厚朴酚抑制变形链球菌及釉质碳酸脱矿的研究[J]. 口腔医学研究, 2018, 34(7): 788-792.
[12]	王欣,张程,李天成,陶贵渝,段沛沛,邹淑娟. 一种新型牙骨质细胞系的矿化特性研究[J]. 口腔医学研究, 2018, 34(12): 1324-1328.
[13]	王川, 廖海清, 曹正国. 微阵列芯片分析成牙骨质细胞矿化过程中microRNA表达谱的差异[J]. 口腔医学研究, 2017, 33(2): 135-139.
[14]	梁向阳,孔晶晶,李春年,李安. 不同材料在碳酸饮料所致脱矿釉质再矿化中的作用[J]. 口腔医学研究, 2017, 33(12): 1274-1277.
[15]	宋丹丹,方慧敏,曹阳,俞青. Er:YAG激光预备对牙釉质表面性能及树脂粘结强度的影响[J]. 口腔医学研究, 2017, 33(1): 38-41.