兔关节盘穿孔后髁突骨质改建及Sox9、MMP-13、VEGF表达变化的实验研究

doi:10.13701/j.cnki.kqyxyj.2019.10.020

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (10): 994-1000.DOI: 10.13701/j.cnki.kqyxyj.2019.10.020

兔关节盘穿孔后髁突骨质改建及Sox9、MMP-13、VEGF表达变化的实验研究

肖艳菊^1,2, 吴国民^1*, 张瑶瑶¹, 陈楷¹, 王祥伸¹

1. 吉林大学口腔医院口腔颌面外科吉林长春 130021;
2. 吉林省牙发育及颌骨重塑与再生重点实验室吉林长春 130021

收稿日期:2019-01-12 出版日期:2019-10-28 发布日期:2019-10-22
通讯作者: 吴国民,E-mail:guominwu2006@sina.com
作者简介:肖艳菊(1992~ ),女,黑龙江北安人,硕士在读,主要从事颞下颌关节相关疾病的研究。
基金资助:
国家自然科学青年基金(编号:81400532)

Reconstruction of Condylar Bone and Expressions of Sox9, MMP-13, and VEGF following Rabbit Articular Disc Perforation

XIAO Yanju^1,2, WU Guomin^1*, ZHANG Yaoyao¹, CHEN Kai¹, WANG Xiangshen¹

1. Department of Oral and Maxillofacial Surgery, Stomatology Hospital, Jilin University, Changchun 130021, China;
2. Key Laboratory of Tooth Development and Bone Remodeling and Regeneration, Jilin Province, Changchun 130021, China.

Received:2019-01-12 Online:2019-10-28 Published:2019-10-22

摘要/Abstract

摘要： 目的:分析颞下颌关节骨关节炎中软骨下骨骨质改建情况,检测Sox9、MMP-13、VEGF的表达并探讨它们对软骨下骨骨质改建的作用机制。方法:在兔关节盘的后外侧制备直径为2.5 mm全层穿孔,micro-CT扫描并计算髁突顶部软骨下骨的BV/TV, BS/BV, Tb.Th, Tb.Sp和Tb.N,HE染色及免疫组织化学方法检测髁突中Sox9、MMP-13、VEGF的表达和定位并进行半定量分析。结果:实验组表现为典型的骨关节炎组织病理学特征。实验组软骨下骨BV/TV、Tb.Th和Tb.N明显下降(P＜0.05),BS/BV和Tb.Sp明显上升(P＜0.05)。实验组软骨下骨中Sox9、MMP-13、VEGF的表达量明显高于假手术组(P＜0.05)。结论:软骨下骨改建是TMJOA的一个重要特征,Sox9、MMP-13、VEGF对软骨下骨骨质改建具有重要意义。

关键词: 颞下颌关节, 骨关节炎, 关节盘穿孔, 软骨下骨

Abstract: Objective: To analyze the reconstruction of subchondral bone of TMJOA, to investigate the expression of Sox9, MMP-13, and VEGF, and to explore the mechanism of their effects on subchondral bone reconstruction. Methods: A full-thickness perforation in the diameter of 2.5 mm was made on the posterolateral side of the articular disc. BV/TV, BS/BV, Tb.Th, Tb.Sp, and Tb.N of subchondral bone were calculated by micro-CT. HE staining and immunohistochemical staining was carried out to analyze the expression and localization of Sox9, MMP-13, and VEGF, and semi-quantitative analysis was carried out. Results: Experimental group (EG) showed typical histopathological features of OA. BV/TV, Tb.Th, and Tb.N of EG were significantly decreased (P＜0.05), and BS/BV and Tb.Sp of EG were significantly increased (P＜0.05). The expression of Sox9, MMP-13, and VEGF in the subchondral bone of EG were significantly higher than those of sham operation group (SG) (P＜0.05). Conclusion: The reconstruction of subchondral bone is an important feature of TMJOA. Sox9, MMP-13, and VEGF play an important role in bone remodeling.

Key words: temporomandibular joint, osteoarthritis, perforation of articular disc, subchondral bone

肖艳菊, 吴国民, 张瑶瑶, 陈楷, 王祥伸. 兔关节盘穿孔后髁突骨质改建及Sox9、MMP-13、VEGF表达变化的实验研究[J]. 口腔医学研究, 2019, 35(10): 994-1000.

XIAO Yanju, WU Guomin, ZHANG Yaoyao, CHEN Kai, WANG Xiangshen. Reconstruction of Condylar Bone and Expressions of Sox9, MMP-13, and VEGF following Rabbit Articular Disc Perforation[J]. Journal of Oral Science Research, 2019, 35(10): 994-1000.

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兔关节盘穿孔后髁突骨质改建及Sox9、MMP-13、VEGF表达变化的实验研究

Reconstruction of Condylar Bone and Expressions of Sox9, MMP-13, and VEGF following Rabbit Articular Disc Perforation

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