Poly(VBA-co-VBTAC)/XF-70的制备及抑菌作用的研究

doi:10.13701/j.cnki.kqyxyj.2020.03.016

口腔医学研究 ›› 2020, Vol. 36 ›› Issue (3): 255-258.DOI: 10.13701/j.cnki.kqyxyj.2020.03.016

Poly(VBA-co-VBTAC)/XF-70的制备及抑菌作用的研究

王岩松, 刘建林^*, 陈晓霞

广州中医药大学深圳医院广东深圳 518034

收稿日期:2019-06-20 出版日期:2020-05-15 发布日期:2020-05-15
通讯作者: 刘建林,E-mail:wangyansong15985@163.com
作者简介:王岩松(1982~),男,辽宁沈阳人,硕士,主治医师,主要从事种植修复临床治疗工作。
基金资助:
福田区卫生公益性科研项目(编号:FTWS2017001)

Preparation and Bacteriostasis Effects of Poly(VBA-co-VBTAC)/XF-70

WANG Yansong, LIU Jianlin^*, CHEN Xiaoxia

Shenzhen Hospital affiliated to Guangzhou University of Traditional Chinese Medicine, Shenzhen 518034, China

Received:2019-06-20 Online:2020-05-15 Published:2020-05-15

摘要/Abstract

摘要： 目的：评估聚(4-乙烯基苯甲酸共(ar-乙烯基苄基)三甲基氯化铵Poly(VBA-co-VBTAC)/XF-70纳米纤维的理化性质,探讨其抑制细菌生物膜形成作用。方法：制备与Poly(VBA-co-VBTAC)／XF-70纳米纤维,扫描电镜(SEM)观察其微观形貌。使用高效液相(HPLC)测试释放曲线,建立金黄色葡萄球菌、牙龈卟啉单胞菌生物膜的体外模型。取1.00 mg/L的XF-70和相同XF-70含量的Poly(VBA-co-VBTAC)／XF-70进行抑菌试验。结果：XF-70在300 min内的释放量约为80%,XF-70组和Poly(VBA-co-VBTAC)／XF-70组细菌生物膜形成量显著降低,Poly(VBA-co-VBTAC)／XF-70的抑菌效果显著优于XF-70(P<0.05)。结论：Poly(VBA-co-VBTAC)／XF-70有良好的释放曲线,可显著提高XF-70对金黄色葡萄球菌、牙龈卟啉单胞菌生物膜形成的抑制作用。

关键词: Poly(VBA-co-VBTAC), XF-70, 纳米纤维, 抑菌作用

Abstract: Objective: To evaluate the physicochemical properties of poly (4-vinyl benzoic acid co (ar-vinyl benzyl) trimethylammonium chloride poly (VBA-co-VBTAC)/XF-70 nanofibers, and to explore their inhibitory effects on the formation of bacterial biofilms. Methods: Poly (VBA-co-VBTAC) nanofibers were prepared and compared with Poly (VBA-co-VBTAC)/XF-70. The morphology of the nanofibers was observed by SEM. The release curves of Staphylococcus aureus and Porphyromonas gingivalis biofilms were measured by high-performance liquid chromatography (HPLC), and the in vitro models of Staphylococcus aureus and Porphyromonas gingivalis biofilms were established. Bacterial inhibition tests were carried out on 1.00 mg/L XF-70 and Poly (VBA-co-VBTAC)/XF-70 with the same content of XF-70, respectively. Results: The release of XF-70 within 300 minutes was nearly 80%. The biofilm formation (absorbance value) of the two bacteria decreased significantly, and the bacteriostasis effect of Poly (VBA-co-VBTAC)/XF-70 was significantly better than that of XF-70 group (P<0.05). Conclusion: Poly (VBA-co-VBTAC)/XF-70 has a good short-term release curve, which can improve the inhibition of XF-70 on the formation of biofilm of Staphylococcus aureus and Porphyromonas gingivalis.

Key words: poly(VBA-co-VBTAC), XF-70, nanofibers, bacteriostasis

王岩松, 刘建林, 陈晓霞. Poly(VBA-co-VBTAC)/XF-70的制备及抑菌作用的研究[J]. 口腔医学研究, 2020, 36(3): 255-258.

WANG Yansong, LIU Jianlin, CHEN Xiaoxia. Preparation and Bacteriostasis Effects of Poly(VBA-co-VBTAC)/XF-70[J]. Journal of Oral Science Research, 2020, 36(3): 255-258.

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Poly(VBA-co-VBTAC)/XF-70的制备及抑菌作用的研究

Preparation and Bacteriostasis Effects of Poly(VBA-co-VBTAC)/XF-70

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