根管生物膜体外模型研究进展

doi:10.13701/j.cnki.kqyxyj.2024.02.003

口腔医学研究 ›› 2024, Vol. 40 ›› Issue (2): 108-112.DOI: 10.13701/j.cnki.kqyxyj.2024.02.003

根管生物膜体外模型研究进展

鲍萍萍^1,2, 邓嘉胤^3*

1.天津市口腔医院第一门诊部,南开大学医学院天津 300041;
2.天津市口腔功能重建重点实验室天津 300041;
3.天津医科大学口腔医学院天津 300070

收稿日期:2023-03-10 出版日期:2024-02-28 发布日期:2024-02-26
通讯作者: *邓嘉胤,E-mail:jdeng@tmu.edu.cn
作者简介:鲍萍萍(1979~ ),女,河北人,硕士,主任医师,主要从事牙体牙髓病学临床工作与基础研究。
基金资助:
天津市卫生健康科技重点项目(编号:TJWJ2021ZD009) 天津市医学重点学科(专科)建设项目(编号:TJYXZDXK-078D) 天津市口腔功能重建重点试验室面上项目(编号:2021KLMS04)

Research Progress on Root Canal Biofilm Models In Vitro

BAO Pingping^1,2, DENG Jiayin^3*

1. The First Clinical Division, Tianjin Stomatological Hospital, School of Medicine, Nankai University, Tianjin 300041, China;
2. Tianjin Key Laboratory of Oral and Maxillofacial Function Reconstruction, Tianjin 300041, China;
3. School of Stomatology, Tianjin Medical University, Tianjin 300070, China

Received:2023-03-10 Online:2024-02-28 Published:2024-02-26

摘要/Abstract

摘要： 细菌生物膜是导致牙髓根尖周病及根管治疗失败的主要因素。建立体外根管生物膜模型是根管感染控制研究中的重要环节,模型的选择、设计与构建是否科学严谨直接影响研究的结果与意义。本文就近年来体外研究中常用的根管生物膜模型进行综述,讨论模型构建的各项因素,如细菌组成、载体的选择和预处理、细菌接种和培养条件等,以期为根管感染控制研究中选择和设计更科学、合理的模型,研发更能模拟体内情况、稳定可重复的标准模型提供参考。

关键词: 根管生物膜, 实验模型, 根管感染, 根管微生物

Abstract: Bacterial biofilm is the main cause of pulpal/periapical diseases and endodontic treatment failures. The establishment of in vitro root canal biofilm models is an important part of root canal infection control studies. Whether the selection, design, and construction of the models are scientific and rigorous directly affects the results and significance of the studies. This paper reviews the endodontic biofilm models commonly used in latest in vitro studies, and discusses the various constitutive factors, such as the microbial composition, the selection and pre-treatment of substrates, and the inoculation and growth conditions, hoping to provide a reference for the selection and design of more scientific and sound models and the development of more stable and reproducible standardized models that can better mimic the in vivo conditions for future research.

Key words: root canal biofilm, experimental model, endodontic infection, root canal microbiome

鲍萍萍, 邓嘉胤. 根管生物膜体外模型研究进展[J]. 口腔医学研究, 2024, 40(2): 108-112.

BAO Pingping, DENG Jiayin. Research Progress on Root Canal Biofilm Models In Vitro[J]. Journal of Oral Science Research, 2024, 40(2): 108-112.

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根管生物膜体外模型研究进展

Research Progress on Root Canal Biofilm Models In Vitro

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