口腔医学研究

口腔医学研究 ›› 2024, Vol. 40 ›› Issue (4): 310-314.DOI: 10.13701/j.cnki.kqyxyj.2024.04.006

• 口腔生物学研究 • 上一篇    下一篇

依普黄酮促进人牙髓干细胞增殖和成骨分化

乐曼妮1,2, 王小聪1,2, 黄子璇1,2, 张慧琳1,2, 张晓月1,2, 赵卿1,2, 李明1,2*, 王基栋3*   

  1. 1.湖南中医药大学口腔医(学)院 湖南 长沙 410208;
    2.长沙市口腔医院 湖南 长沙 410004;
    3.中南大学湘雅医学院附属常德医院 湖南 常德 415000
  • 收稿日期:2023-11-23 发布日期:2024-04-22
  • 通讯作者: *李明,E-mail:liming@hnucm.edu.cn;王基栋,E-mail:wangjidong0212@163.com
  • 作者简介:乐曼妮(1999~ ), 女, 湖南永州人, 硕士在读, 研究方向:牙髓干细胞。
  • 基金资助:
    湖南省自然科学基金面上基金项目(编号:2022JJ30630)湖南省科技厅临床医疗技术创新引导项目(编号:2021SK53301)湖南省卫生健康委员会重点指导课题(编号:202108051626)湖南省教育厅重点项目(编号:22A0249)湖南省重点研发计划项目(编号:2020SK2137)长沙市自然科学基金(编号:kq2208458)

Ipriflavone Promotes Proliferation and Mineralization of Human Dental Pulp Stem Cells

LE Manni1,2, WANG Xiaocong1,2, HUANG Zixuan1,2, ZHANG Huilin1,2, ZHANG Xiaoyue1,2, ZHAO Qing1,2, LI Ming1,2*, WANG Jidong3*   

  1. 1. School of Stomatology, Hunan University of Traditional Chinese Medicine, Changsha 410208, China;
    2. Changsha Stomatological Hospital, Changsha 410004, China;
    3. Changde Hospital, Xiangya Medical College, Central South University, Changde 415000, China
  • Received:2023-11-23 Published:2024-04-22

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摘要： 目的: 初步检测依普黄酮(IP)对人牙髓干细胞(human dental pulp stem cells,hDPSCs)的增殖和成骨向分化的影响。方法: 在体外对hDPSCs进行培养鉴定,用含IP(10-9~10-5 mol/L)的完全培养液培养hDPSCs,CCK-8法检测不同时间点(1、2、3 d)的细胞活性;用含IP(10-8~10-5 mol/L)的矿化诱导液诱导hDPSCs 7 d,通过碱性磷酸酶活性测定、ALP染色、茜素红染色及RT-qPCR检测IP对hDPSCs成骨分化的影响。结果: CCK-8检测结果表明10-9~10-5 mol/L IP均可促进hDPSCs增殖,其中10-6 mol/L IP组促进增殖效果最佳(P<0.05);10-6 mol/L IP组ALP染色加深,ALP活性增高(P<0.05),矿化结节增多(P<0.05); RT-qPCR检测结果显示10-6 mol/L IP组能够提高成骨分化相关基因骨钙素(osteocalcin,OCN)、碱性磷酸酶和矮小相关转录基因2的表达水平(P<0.05)。结论: 10-6 mol/L IP能提高hDPSCs增殖和成骨向分化的能力。

关键词: 牙髓干细胞, 增殖, 成骨分化, 依普黄酮

Abstract: Objective: To investigate the effects of ipriflavone (IP) on proliferation and mineralization of human dental pulp stem cells (hDPSCs). Methods: The hDPSCs were cultured in complete culture medium containing IP (10-9-10-5 mol/L) and identified. The cell activity at different time points (1, 2, 3 d) was detected by CCK-8. After induced for 7 days with mineralization liquid containing IP (10-8-10-5 mol/L), the alkaline phosphatase (ALP) activity, ALP staining, alizarin red staining, and RT-qPCR were used to detect the osteogenic differentiation of hDPSCs. Results: CCK-8 detection showed that 10-9-10-5 mol/L IP could promote the proliferation of hDPSCs,and 10-6 mol/L IP had the best results (P<0.05). In 10-6 mol/L IP group, the ALP staining was deepened, the activity was increased (P<0.05), and the mineralization nodules were increased. RT-qPCR showed that the contents of Runt-related transcription factor, ALP, and osteocalcin in the 10-6 mol/L IP group were significantly up-regulated (P<0.05). Conclusion: 10-6 mol/L IP can promote the proliferation and mineralization of hDPSCs.

Key words: dental pulp stem cells, multiplication, differentiation of bone, ipriflavone