口腔医学研究

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (5): 441-443.

• 基础研究论著 • 上一篇    下一篇

Bio-oss骨粉复合HIF-1α蛋白对拔牙创骨愈合影响的实验研究

谭鸾君, 阮征, 张劲娥, 黄远亮, 王磊*   

  1. 同济大学附属东方医院口腔科 上海 200120
  • 收稿日期:2014-04-24 出版日期:2015-05-28 发布日期:2016-04-29
  • 通讯作者: 王磊, E-mail:13761110551@163.com
  • 作者简介:谭鸾君(1982~ ),女,山西人,住院医师,硕士,主要从事口腔科的临床治疗工作。
  • 基金资助:
    浦东新区卫生局青年项目(PW2011B-13);东方启航计划面上项目(DFQH-M101);浦东新区科技发展基金创新资金(PKJ2013-Y15)

An Experimental Study of Bio-oss with HIF-1α Protein on Tooth Extraction Bone Healing.

TAN Luan-jun, RUAN Zheng, ZHANG Jin-e, et al.   

  1. Department of Stomatology, East Hospital Tongji University, Shanghai 200120
  • Received:2014-04-24 Online:2015-05-28 Published:2016-04-29

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摘要： 目的:研究Bio-oss骨粉复合HIF-1α蛋白对拔牙创骨愈合的影响。方法:全麻下完整拔除3只Beagle犬的双侧下颌二、四前磨牙近中根,随机选择一个牙槽窝作为空白对照组,其余即刻分别植入bio-oss骨粉、bio-oss复合浓度为100 μg/L的HIF-1α蛋白,分别与术后6、12周取材,进行Micro-CT扫描、硬组织切片染色,观察拔牙创的骨愈合情况和组织病理学变化。结果:Micro-ct扫描显示,第6周时各组骨小梁数目、骨密度和BV/TV均有一定的差别。第12周时, bio-oss复合浓度HIF-1α组与bio-oss骨粉组、空白组差别显著(P<0.05),差别有统计学意义。硬组织切片显示第12周时,bio-oss复合HIF-1α组有良好的新骨形成。结论:Bio-oss骨粉复合HIF-1α蛋白骨修复材料可以促进拔牙创的愈合。

关键词: Bio-oss骨粉, HIF-1α蛋白, 拔牙创, 骨愈合

Abstract: Objective: To study Bio-oss bone with HIF-1α protein on tooth extraction bone healing. Methods: Under general anesthesia, mesial root of the 2nd, 4th premolars in both sides of mandible of 3 Beagle dogs were extracted. Randomly, a wound was selected as the blank control group, and the rest were immediately implanted bio-oss, bio-oss with HIF-1α protein of 100ng/ml. After six and twelve weeks, the beagle dogs were sacrificed. The gross speciments were examined by Micro-CT images, and mineralized specimen preparations were prepared. Results: Micro-CT scan analysis showed that each group had some differences in the number of trabecular, bone mineral density and BV/TV. After twelve weeks,bio-oss with HIF-1α group was significantly higher than the bio-oss group、 the control group (P<0.05). The difference was statistical significance. Tissue slides also revealed an effective osteogensis in those wounds implanted with bio-oss with HIF-1α. Conclusion: Bio-oss with HIF-1α protein l can promote the regeneration of alveolar bone.

Key words: Bio-oss , HIF-1α protein, Tooth extraction, Bone healing

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