口腔医学研究

口腔医学研究 ›› 2020, Vol. 36 ›› Issue (3): 251-254.DOI: 10.13701/j.cnki.kqyxyj.2020.03.015

• 修复材料抗菌性研究 • 上一篇    下一篇

IL-6抑制剂控制种植体愈合期炎症性骨吸收的动物实验研究

余科1*, 贺晓萍2, 文才1, 刘旭琳1, 杨健1   

  1. 1. 西南医科大学附属口腔医院种植科 四川 泸州 646000;
    2. 西南医科大学附属口腔医院修复科 四川 泸州 646000
  • 收稿日期:2019-07-18 出版日期:2020-05-15 发布日期:2020-05-15
  • 通讯作者: 余科,E-mail:65968999@qq.co
  • 作者简介:余科(1979~),男,四川崇州人,讲师,博士,主要从事口腔种植基础与临床研究。
  • 基金资助:
    泸州市科技项目[编号:2016-S-66(2/3)];中华口腔医学会西部口腔医学临床科研基金项目(编号:CSA-W2018-03)

Study on IL-6 Inhibitor Controlling Inflammatory Bone Absorption during Implant Healing in Rabbits

YU Ke1*, HE Xiaoping2, WEN Cai1, LIU Xulin1, YANG Jian1   

  1. 1. Department of Implantology, Stomatology Hospital Affiliated to Southwest Medical University, Luzhou 646000, China;
    2. Department of Prosthodontics, Stomatology Hospital Affiliated to Southwest Medical University, Luzhou 646000, China
  • Received:2019-07-18 Online:2020-05-15 Published:2020-05-15

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摘要： 目的：探索IL-6抑制剂在控制种植体愈合期炎症性骨吸收中的效果,为临床种植体炎症性骨吸收的治疗提供新的思路。方法：选择健康成年雄性新西兰大白兔20只,拔除双侧下颌前牙,3月后于下前牙区常规植入登腾微型种植体(2.5×12 mm)一枚,随机分为4组。A组种植后不做处理,另外3组用两根4-0丝线栓丝在种植体颈部;其中B组栓丝后不做处理,C组栓丝后即刻在种植体颈部注入盐酸米诺环素软膏,D组在种植后即刻及1周后按8 mg/kg静脉注射IL-6抑制剂Tocilizumab。8周后处死动物,收集种植体周围骨组织行X线片及micro-CT扫描。结果：X片可见A、D组种植体周围无明显骨吸收,B组垂直吸收约3~4个种植体螺纹,C组吸收约1~2个螺纹。Micro-CT分析可见界定范围内D组骨体积率最高,A组与C组次之,B组最低,差别有统计学意义。结论：IL-6抑制剂Tocilizumab可控制种植体愈合期炎症性骨吸收,可增加种植体周围骨体积率。

关键词: IL-6抑制剂, 牙种植体, 骨愈合期, 骨吸收, 原位动物模型

Abstract: Objective: To explore the effect of IL-6 inhibitor on the control of inflammatory bone resorption during implant healing, and to provide new ideas for the treatment of inflammatory bone resorption around implants in clinic. Methods: Twenty healthy adult male New Zealand white rabbits were randomly divided into four groups, and the bilateral mandibular anterior teeth of these rabbits were extracted. Three months later, 20 implants (2.5×12 mm, Dentium) were routinely placed into the lower anterior teeth area. Group A was blank control, and the implants of the other three groups were ligated with two 4-0 thread bolts. Group B was control with no other treatment. In group C, minocycline hydrochloride ointment was injected into the gingiva around the implants, and in group D, tocilizumab was injected intravenously at 8 mg/kg immediately and 1 week after implantation. Eight weeks later, animals were sacrificed, and bone tissues around implants were collected for X-ray and micro-CT scanning. Results: X-ray showed that there was no obvious bone absorption around the implants in group A and D, about 3-4 implant threads were exposed in group B, and about 1-2 threads were exposed in group C. Micro-CT analysis showed that the highest bone volume rate was found in group D, followed by group A and group C, and the lowest in group B (P<0.05). Conclusion: IL-6 inhibitor tocilizumab can control inflammatory bone resorption during implant healing and increase bone volume ratio around implants.

Key words: IL-6 inhibitors, dental implant, bone healing stage, bone resorption, orthotopic animal model