口腔医学研究

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (5): 471-475.DOI: 10.13701/j.cnki.kqyxyj.2019.05.014

• 牙周病学研究 • 上一篇    下一篇

β-隐黄素对大鼠实验性牙周炎炎症因子的影响

李冬雪1, 王国芳1#*, 崔晓宇2, 李小红3, 轩亚茹4, 王守儒5   

  1. 1. 郑州大学第一附属医院口腔科 河南 郑州 450052;
    2. 濮阳市油田总医院口腔科 河南 濮阳 457001;
    3. 安阳市第六人民医院安阳市口腔医院口腔科 河南 安阳 455000;
    4. 郑州人民医院口腔科 河南 郑州 450053;
    5. 河南省中医院口腔科 河南 郑州 450002
  • 收稿日期:2018-09-26 出版日期:2019-05-28 发布日期:2019-05-21
  • 通讯作者: 王国芳,E-mail: wgfdxz2003@163.com
  • 作者简介:李冬雪(1992~ ),女,吉林人,硕士在读,研究方向:牙周病病因及临床研究。 王国芳(1976~ ),女,河南人,讲师,博士,主要从事牙周病的病因研究及临床治疗。
  • 基金资助:
    王守儒全国名老中医药专家传承工作室

Effect of β-cryptoxanthin on Level of Inflammatory Factors in Experimental Periodontitis.

LI Dong-xue1, WANG Guo-fang1#*, CUI Xiao-yu2, LI Xiao-hong3, XUAN Ya-ru4, WANG Shou-ru5   

  1. 1. Department of Periodontology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, China;
    2. Department of Stomatology, Puyang Oilfield General Hospital, Puyang 457001, China;
    3. Department of Stomatology, Anyang Sixth People's Hospital (Anyang Stomatological Hospital), Anyang 455052, China;
    4. Department of Stomatology, People's Hospital of Zhengzhou, Zhengzhou 450012, China;
    5. Department of Stomatology, Henan Provincial Hospital of Traditional Chinese Medicine, Zhengzhou 450000, China.
  • Received:2018-09-26 Online:2019-05-28 Published:2019-05-21

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摘要： 目的: 研究β-隐黄素对大鼠实验性牙周炎炎症因子的影响并探讨其相关作用机制。方法: 30只雄性SD大鼠随机分为正常组(N组)、牙周炎组(P组)、β-隐黄素干预组(E组),每组10只。采取结扎双侧上颌第二磨牙颈部联合注射脂多糖(lipopolysaccharide,LPS)诱导牙周炎模型。E组同时于相同位点注射β-隐黄素(每只12 μL),每48 h注射1次,共3次。实验第7天采血并立即分离双侧上颌骨,右侧颌骨行组织学分析,血清及左侧分离的牙龈组织以酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)法检测细胞白介素-1β(interleukin-1β ,IL-1β)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、前列腺素E2(prostaglandin E2,PGE2)含量。结果: E组和P组大鼠IL-1β、TNF-α、PGE2水平明显高于N组,且炎症细胞浸润较多,牙槽骨吸收明显。与P组比较,E组中IL-1β、TNF-α、PGE2水平明显降低(P<0.05),炎症细胞浸润较少,牙槽骨吸收减轻。结论: β-隐黄素可能通过降低组织中的炎症因子水平,减少炎症细胞浸润,从而减轻牙周组织的炎症反应,减少牙槽骨吸收。

关键词: β-隐黄素, 牙周炎, 炎症因子, 骨吸收

Abstract: Objective: To study the effect of β-cryptoxanthin on inflammatory factors in rats with experimental periodontitis and to explore its mechanism. Methods: 30 male Sprague-Dawley rats were randomly divided into normal group (group N), periodontitis group (group P), and β-cryptoxanthin intervention group (group E), with 10 rats of each. Experimental periodontitis model was induced by ligation of bilateral maxillary second molars cervix and injection with lipopolysaccharide (LPS). At the same time, β-cryptoxanthin (12μl/each) was injected in group E at the same site. All groups were treated every 48 hours for three times. Blood was collected and the bilateral maxilla were immediately separated on day 7, the right part of the tissues were subjected to histological analysis, serum and the left gingival tissues were detected by ELISA for IL-1β, TNF-α, and PGE2. Results: The levels of IL-1β, TNF-α and PGE2 in group E and group P were higher than those in group N, the infiltration of inflammatory cells was increased and the alveolar bone absorption was obvious. Compared with group P, the levels of IL-1β, TNF-α, and PGE2 in group E were significantly lower, the infiltration of inflammatory cells was less, and the absorption of alveolar bone was mild. Conclusion: β-cryptoxanthin can decrease the level of inflammatory factors and the inflammatory cells infiltration in tissues, thereby reducing the inflammation of periodontaltissues and alleviating alveolar bone resorption.

Key words: β- cryptoxanthin, Periodontitis, Inflammatory factors, Bone resorption