抗中间普氏菌卵黄抗体对大鼠妊娠期龈炎疗效的初探

doi:10.13701/j.cnki.kqyxyj.2019.05.013

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (5): 466-470.DOI: 10.13701/j.cnki.kqyxyj.2019.05.013

抗中间普氏菌卵黄抗体对大鼠妊娠期龈炎疗效的初探

蒋鹏¹, 徐燕^1*, 周莉莉¹, 徐涵颖¹, 辛保见¹, 叶兴如¹, 沈继龙³, 程婷²

1. 安徽医科大学口腔医学院,安徽医科大学附属口腔医院, 安徽省口腔疾病研究重点实验室安徽合肥 230032;
2. 安徽安科生物工程集团股份有限公司安徽合肥 230032;
3. 安徽医科大学人兽共患病安徽省重点实验室安徽合肥 230032

收稿日期:2018-10-18 出版日期:2019-05-28 发布日期:2019-05-21
通讯作者: 徐燕,E-mail:173236344@qq.com
作者简介:蒋鹏(1990~ )男,浙江温岭人,硕士,主要从事牙周临床工作。
基金资助:
安徽省教育厅自然科学重大项目(编号:KJ2017ZD17)

Preliminary Study on Effect of Yolk Antibody against Prevotella Intermedia on Gingivitis during Pregnancy in Rats.

JIANG Peng¹, XU Yan^1*, ZHOU Li-li¹, XU Han-ying¹, XIN Bao-jian¹, YE Xin-ru¹, SHEN Ji-long³, CHENG Ting²

1. College &Hospital of Stomatology, Anhui Medical University, Key Lab. of Oral Diseases Research of Anhui Province, Hefei 230032, China;
2. Anhui Anke Biotechnology (Group) Co.,Ltd, Hefei 230032, China;
3. Anhui Key Laboratory of Zoonoses, Anhui Medical University, Hefei 230032, China.

Received:2018-10-18 Online:2019-05-28 Published:2019-05-21

摘要/Abstract

摘要： 目的: 制备和纯化抗中间普氏菌特异性卵黄免疫球蛋白(IgY),并评价其对实验性妊娠期大鼠牙龈炎的治疗作用。方法: 两步硫酸铵沉淀法获得特异性IgY,将24只雌性SD大鼠随机分为4组,A组(空白组)、B组(阴性组):造模、C组:(阳性组)造模＋替硝唑溶液、D组:(实验组)造模＋IgY溶液,通过牙龈指数(GI)、菌斑指数(PLI)、体重变化、RT-qPCR,及组织病理学切片观察特异性IgY的治疗作用。结果: 通过对大鼠实验性妊娠期牙龈炎的治疗GI、PLI(P＜0.001)、体重(P＜0.05)均得到改善,龈下菌斑治疗前后中间普氏菌的构成比明显降低(P＜0.05)。结论: 抗中间普氏菌特异性卵黄抗体可改善大鼠实验性妊娠期的牙龈炎症状况。

关键词: 中间普氏菌, 牙龈炎, 实时荧光定量PCR

Abstract: Objective: To prepare and purify specific yolk immunoglobulin (IgY) against Prevotella intermedia(P.intermedia, Pi)and to evaluate its therapeutic effect on experimental gingivitis in pregnant rats. Methods: Specific IgY was prepared by two-step ammonium sulfate precipitation method. Twenty-four female SD rats were randomly divided into 4 groups: group A (blank control group), group B (negative group): modeled, group C (positive control group): modeled + tinidazole solution, and group D (experimental group): modeled + specific IgY solution. The therapeutic effect of specific IgY was observed by gingival index (GI), plaque index (PLI), body weight change, RT-qPCR, and histopathological sections. Results: Through the treatment of experimental gestational gingivitis in rats, the GI, PLI (P<0.001) and body weight (P<0.05) were improved, and the composition ratio of P. intermedia was significantly decreased after the treatment of subgingival plaque (P<0.05). Conclusion: Anti-P.intermedia--specific yolk antibody can relief the gingival inflammation in rats during pregnancy.

Key words: Prevotella intermedia, Gingivitis, Real-time quantitative polymerase chain reaction

蒋鹏, 徐燕, 周莉莉, 徐涵颖, 辛保见, 叶兴如, 沈继龙, 程婷. 抗中间普氏菌卵黄抗体对大鼠妊娠期龈炎疗效的初探[J]. 口腔医学研究, 2019, 35(5): 466-470.

JIANG Peng, XU Yan, ZHOU Li-li, XU Han-ying, XIN Bao-jian, YE Xin-ru, SHEN Ji-long, CHENG Ting. Preliminary Study on Effect of Yolk Antibody against Prevotella Intermedia on Gingivitis during Pregnancy in Rats.[J]. Journal of Oral Science Research, 2019, 35(5): 466-470.

参考文献

[1] Usin MM, Tabares SM, Parodi RJ, et al. Periodontal conditions during the pregnancy associated with periodontal pathogens [J]. J Investig Clin Dent, 2013, 4(1):54-59.
[2] Puertas A, Magan-Fernandez A, Blanc V, et al. Association of periodontitis with preterm birth and low birth weight: a comprehensive review [J]. J Matern Fetal Neonatal Med, 2018, 31(5):597-602.
[3] 陈智慧,吕莉娟,张亮,等.口腔微生物与不良妊娠结局关系的研究进展[J].口腔医学研究,2017,33(8):905-908.
[4] Mallorquí-Fernández N,Manandhar SP,Mallorquí-Fernández G.A new autocatalytic activation mechanism for cysteine proteases revealed by Prevotella intermedia interpain A [J]. J Biol Chem,2007,283(5):2871-2882.
[5] Potempa M, Potempa J, Kantyka T, et al. Interpain A, a cysteine proteinase from Prevotella intermedia, inhibits complement by degrading complement factor C3 [J]. PLoS Pathog, 2009,5(2):e1000316.
[6] Byrne DP, Potempa J, Olczak T,et al.Evidence of mutualism between two periodontal pathogens: Cooperative haem acquisition by the HmuY haemophore of Porphyromonas gingivalis and the cysteine protease interpain A (InpA) of Prevotella intermedia [J]. Mol Oral Microbiol, 2013, 28(3):219-229.
[7] 孟明理,徐燕,王晓静,等.抗牙龈素粘附片段Hgp44卵黄抗体对实验性牙周炎的抑制作用[J].口腔医学研究,2016,32(7):699-703.
[8] Hou YY, Zhen YH, Wang D, et al. Protective effect of an egg yolk-derived immunoglobulin (IgY) against Prevotella intermedia-mediated gingivitis[J].J Appl Microbiol, 2014, 116(4):1020-1027.
[9] Štefan M, Vojtěch J. Antibiotic therapy in pregnancy [J]. Ceska Gynekol, 2018, 83(1):70-80.
[10] 王晓静,徐燕,孟明理,等.抗中间普氏菌卵黄抗体的制备及其对实验性妊娠期龈炎的预防作用[J].安徽医科大学学报,2017,52(8):1154-1159.
[11] Akbari MR, Ahmadi A, Mirkalantari S. Anti-vibriocholerae IgY antibody inhibits mortality in suckling mice model [J]. J Natl Med Assoc, 2018, 110(1):84-87.

抗中间普氏菌卵黄抗体对大鼠妊娠期龈炎疗效的初探

Preliminary Study on Effect of Yolk Antibody against Prevotella Intermedia on Gingivitis during Pregnancy in Rats.

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 10

编辑推荐

Metrics

[1]	叶兴如, 徐燕, 庞罡, 王莹, 周莉莉, 蒋鹏, 沈继龙, 王荣海. RT-qPCR检测CPC对中重度慢性牙周炎患者口内环境中红色复合体的影响[J]. 口腔医学研究, 2018, 34(8): 852-856.
[2]	程孟文，周毅. MiR-34a在牙周膜细胞成骨向分化中的作用[J]. 口腔医学研究, 2017, 33(9): 928-932.
[3]	杜建东,郑雪莉,杨兰,张颖杰,韩保迪. 中药大黄对正畸固定矫治中龈炎的治疗研究[J]. 口腔医学研究, 2016, 32(8): 832-835.
[4]	谢沛原,余占海,朱玉娟,李志强,周建业. 4311名不同民族大学新生口腔健康状况调查研究[J]. 口腔医学研究, 2015, 31(9): 860-862.
[5]	热孜万·克衣木,李泽慧,米丛波. 不同民族固定矫治所致牙龈炎和前牙牙龈增生的临床研究[J]. 口腔医学研究, 2015, 31(9): 913-916.
[6]	刘崇武,吴春芳,骆凯,王希,陈超,郑瑜谦. 不同牙周状态下龈沟液中瘦素水平的比较[J]. 口腔医学研究, 2015, 31(8): 798-.
[7]	韩祥祯,魏薇,何惠宇,胡杨,王欢欢,邬薇薇. bFGF重组慢病毒对羊骨髓间充质干细胞成骨功能影响的研究[J]. 口腔医学研究, 2015, 31(7): 649-652.
[8]	王欢欢, 沈玉凤, 胡杨, 韩祥祯, 董红宾, 何惠宇. 牙胚细胞与骨形态发生蛋白4转染的骨髓间充质干细胞相互诱导的体外实验研究[J]. 口腔医学研究, 2015, 31(3): 237-241.
[9]	袁杰,王少果,周建业,吾斯曼江艾尔肯,何祥一,李志强. 慢性牙周炎唾液牙龈卟啉单胞菌的定量PCR检测[J]. 口腔医学研究, 2015, 31(2): 133-136.
[10]	王欢欢,胡杨,韩祥祯,邬薇薇,何惠宇. 骨形态发生蛋白4慢病毒载体构建及对鼠骨髓间充质干细胞成牙功能影响[J]. 口腔医学研究, 2015, 31(1): 7-10.