口腔医学研究

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (8): 852-856.DOI: 10.13701/j.cnki.kqyxyj.2018.08.013

• 牙周病学研究 • 上一篇    下一篇

RT-qPCR检测CPC对中重度慢性牙周炎患者口内环境中红色复合体的影响

叶兴如1, 徐燕1*, 庞罡1, 王莹1, 周莉莉1, 蒋鹏1, 沈继龙2, 王荣海3   

  1. 1. 安徽医科大学口腔医学院,安徽医科大学附属口腔医院, 安徽省口腔疾病研究中心实验室 安徽 合肥 230032;
    2. 安徽医科大学人兽共患病安徽省重点实验室 安徽 合肥 230032;
    3. 安徽安科生物工程(集团)股份有限公司 安徽 合肥 230032
  • 收稿日期:2017-12-11 出版日期:2018-08-28 发布日期:2018-08-23
  • 通讯作者: 徐燕,E-mail: 173236344@qq.com
  • 作者简介:叶兴如(1991~ ),女,安徽黄山人,硕士,主要从事牙周临床研究工作。
  • 基金资助:
    安徽省教育厅自然科学重大项目(编号:KJ2017ZD17);安徽省教育厅2015级研究生“千人培养计划”安医大安科生物校企合作项目(编号:K2015011)

Effect of Cetylpyridinium Chloride on Red Complex Bacteria in Moderate to Severe Chronic Periodontitis Patients by Real-time Quantitative PCR

YE Xing-ru1, XU Yan1*, PANG Gang1, WANG Ying1, ZHOU Li-li1, JIANG Peng1, SHEN Ji-long2, WANG Rong-hai3   

  1. 1. Department of Periodontology, Stomatologic Hospital & College, Anhui Medical University. Key Lab of Oral Diseases Research of Anhui Province, Hefei 230032, China;
    2. Key Lab of Zoonoses of Anhui Province, Anhui Medical University, Hefei 230032, China;
    3. Anhui Anke Biotechnology (Group) Co.Ltd, Hefei 230032, China
  • Received:2017-12-11 Online:2018-08-28 Published:2018-08-23

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摘要： 目的:利用RT-qPCR检测CPC对中重度慢性牙周炎患者口内环境中红色复合体构成比的影响。方法:临床选择40例中重度慢性牙周炎患者,随机分成CPC(试验)组、溶媒(对照)组,牙周基础治疗后分别使用该药品含漱+袋内冲洗。记录患者基线和用药4周后的临床指标;采集龈下菌斑、龈沟液和唾液样本,BANA试验检测胰蛋白酶样酶含量,RT-qPCR检测P.gingivalis、T.forsythia、T.denticola在总菌量中的构成比。结果:治疗后:1)试验组AL、BOP、PLI显著改善(P<0.01),对照组仅AL改善(P<0.01);2)两组龈下菌斑和龈沟液样本中胰蛋白酶样酶含量均显著下降(P<0.05);3)两组P.gingivalis构成比均显著下降(P<0.01),T.forsythia在试验组构成比显著下降(P<0.01),而T.denticola治疗前后无变化;4)P.gingivalis、T.forsythia、T.denticola两两之间构成比均显著相关(P<0.01)。结论:CPC可抑制牙菌斑形成,改善患者的临床症状,并对龈下菌斑中的P.gingivalis有一定的抑制作用,临床上可用于辅助治疗中重度慢性牙周炎。

关键词: 牙龈卟啉单胞菌, 福塞坦氏菌, 齿垢密螺旋体, 实时荧光定量PCR

Abstract: Objective: To determine the effect of CPC mouthwash on the proportions of red complex bacteria in patients with moderate to severe chronic periodontitis by RT-qPCR. Methods: Forty patients with periodontitis were randomly assigned to two groups: experimental group (SRP+CPC) and control group (SRP+ placebo). Clinical parameters were recorded at baseline and at 4 weeks. Subgingival plaque, gingival crevicular fluid, and saliva samples were collected, and the content of trypsin-like enzyme was detected using BANA test. RT-qPCR was used to detect the proportions of P.gingivalis, T.forsythia and T.denticola in total bacteria. Results: AL, BOP, and PLI significantly improved (P<0.01) after treatment in the experimental group. Only AL significantly improved in the control group (P<0.01). The level of trypsin-like enzyme in subgingival plaque and gingival crevicular fluid for both groups decreased significantly (P<0.05). The numbers of P. gingivalis decreased significantly in both groups (P<0.01), while T.forsythia decreased significantly in the experimental group (P<0.01). There was no significant difference in the numbers of T.denticola after treatment compared to baseline. There was a significant correlation between the proportions of P.gingivalis, T.forsythia, and T.denticola (P<0.01). Conclusion: CPC can inhibit plaque formation and improve clinical parameters in patients with moderate to severe chronic periodontitis, but has no significant inhibitory effect on P.gingivalis, T.forsythia, and T.denticola.

Key words: Porphyromonas gingivalis, Tannerrella forsythia, Treponema denticola, Real-time quantitative polymerase chain reaction