口腔医学研究

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (7): 690-694.DOI: 10.13701/j.cnki.kqyxyj.2019.07.017

• 口腔颌面外科学研究 • 上一篇    下一篇

降钙素影响拔牙后牙槽骨吸收的动物实验研究

高丽1,2, 高莺2*, 周根祥2, 王璨2, 张晓敏2   

  1. 1. 山西医科大学口腔医学院·口腔医院 山西 太原 030001;
    2. 山西医科大学第一医院口腔科,山西医科大学口腔医学院 山西 太原 030001
  • 收稿日期:2018-11-16 出版日期:2019-07-25 发布日期:2019-07-24
  • 通讯作者: 高莺,E-mail:flygaoying1999@163.com
  • 作者简介:高丽(1990~ ),女,山西朔州人,硕士在读,主要从事口腔颌面外科临床与基础研究
  • 基金资助:
    山西省自然科学基金(编号:2014011045-2)山西医科大学第一医院中青年学术技术带头人培育项目(编号:YD1621)

Effect of Calcitonin on Alveolar Bone Resorption

GAO Li1,2, GAO Ying2*, ZHOU Genxiang2, WANG Can2, ZHANG Xiaomin2   

  1. 1. School and Hospital of Stomatology, Shanxi Medical University, Taiyuan 030001 China;
    2. Department of Stomatology, First Hospital of Shanxi Medical University, Taiyuan 030001, China.
  • Received:2018-11-16 Online:2019-07-25 Published:2019-07-24

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摘要： 目的:探讨局部应用降钙素对大鼠牙槽骨吸收的影响。方法:建立剩余牙槽骨吸收模型,分为空白对照组、明胶海绵组、降钙素+明胶海绵组。术后4周、8周和12周取材,应用CBCT、双能X线、组织学、抗酒石酸酸性磷酸酶 (TRAP)染色、免疫组化检测。结果:4周、8周、12周时,降钙素+明胶海绵组剩余牙槽嵴相对高度、骨密度、骨质量均优于同一时间点的明胶海绵组和空白组,明胶海绵组与空白组之间未发现统计学差异;4周时空白组与明胶海绵组的降钙素受体阳性表达、TRAP染色阳性细胞数、RANKL/OPG比值均高于降钙素+明胶海绵组。结论:降钙素局部作用于大鼠拔牙后牙槽窝可有效减少骨吸收,该作用可能是通过降钙素特异性结合降钙素受体从而抑制破骨细胞实现的

关键词: 降钙素, 牙槽窝, 骨吸收

Abstract: Objective: To evaluate the effect of a local treatment of calcitonin on the alveolar fossa following tooth extraction in rats. Methods: Twenty-seven SD rats were randomly divided into three groups: control group, gelatin sponge group, and calcitonin+gelatin sponge group. The model of residual alveolar bone resorption was established by extracting the left mandibular incisor. Rats were sacrificed at 4, 8, and 12 weeks after operation. The alveolar fossae were assessed by CBCT, dual-energy X-ray, histology, TRAP staining, and immunohistochemistry. Results: At 4, 8 and 12 weeks, calcitonin+gelatin sponge rats showed less resorption of the residual alveolar ridge, better bone mineral density and bone mass than gelatin sponge rats and control rats, however there was no significant difference between gelatin sponge rats and control rats. The positive expression of calcitonin receptor, the number of TRAP-positive cells and RANKL/OPG ratio in the control group and gelatin sponge group were higher than those in the calcitonin+gelatin sponge group at 4 weeks. Conclusion: Local treatment of calcitonin at the site of alveolar fossae following tooth extraction could effectively inhibit the resorption of alveolar bone, which may be mediated through the combination of calcitonin and calcitonin receptor to inhibit osteoclasts.

Key words: calcitonin , bone resorption , alveolar fossa