口腔医学研究

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (5): 563-566.DOI: 10.13701/j.cnki.kqyxyj.2018.05.024

• 其他研究 • 上一篇    下一篇

半导体激光美白程序对牙髓组织中降钙素相关肽和5-羟色胺的影响及意义

李春年   

  1. 河北医科大学口腔医院口腔内科,河北省口腔医学重点实验室 河北 石家庄 050017
  • 收稿日期:2017-11-05 出版日期:2018-05-28 发布日期:2018-05-29
  • 作者简介:李春年(1979~ ),男,河北承德人,硕士,副主任医师,主要从事牙体牙髓病学、牙周病学的临床、教学、科研工作,E-mail:lchunnian@163.com
  • 基金资助:
    河北省医学科学研究重点课题计划(编号:ZL20140043)

Effect of Semiconductor Laser on CGRP and 5-HT of Pulp Tissue during Whitening Procedure.

LI Chun-nian   

  1. Department of Oral medicine,Hospital of Stomatology, Hebei Medical University; the Key Laboratory of Stomatology of Hebei Province, Shijiazhuang 050017, China
  • Received:2017-11-05 Online:2018-05-28 Published:2018-05-29

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摘要： 目的:观察在半导体激光美白程序使用连续频率和间断频率照射大鼠磨牙,观察不同时段牙髓组织中的降钙素相关肽(calcitonin gene-related peptide,CGRP)和5-羟色胺(5-hydroxytryptamine,5-HT)表达的变化,为半导体美白时的牙髓保护提供技术参考。方法: 3月龄SD雄性大鼠随机分为正常组(A组)、半导体激光美白连续频率组(B组)、半导体激光美白间断频率组(C组);B组选择半导体激光美白中的7 W、20 Hz、15 s连续频率照射大鼠上颌第一、二磨牙,术后即刻(B0组)、1 d(B1组)、3 d(B3组)、7 d(B7组)处死大鼠;C组选择半导体激光美白中的7 W、20 Hz、30 s、1/2间断频率照射大鼠上颌第一、二磨牙,术后即刻(C0组)、1 d(C1组)、3 d(C3组)、7 d(C7组)处死大鼠;取大鼠上颌第一、二磨牙制作组织切片,免疫组织化学法检测牙髓组织中CGRP、5-HT的变化。结果:牙髓组织中CGRP、5-HT的表达在B、C各组均高于A组;从术后即刻开始升高,3 d达最高,然后开始降低;相同时段比较B组高于C组。结论:连续频率和间断频率半导体激光美白时,有效时间相同连续频率的刺激性显著高于间断频率,间断频率为首选;同时,激光照射即刻到3 d牙髓组织中疼痛递质表达不断升高,应加强牙髓的保护,减少牙髓的刺激因素。

关键词: 半导体激光, 美白程序, 牙髓组织, 降钙素相关肽, 5-羟色胺

Abstract: Objective: To observe the expression of calcitonin gene-related peptide(CGRP) and 5-hydroxytryptamine(5-HT) in rat molar pulp at different periods during the semiconductor laser whitening using continuous and interval frequencies. Methods: 3-month old male SD rats were randomly divided into group A, group B, and group C. Group B was continuous irradiation in 7 W, 20 Hz, and 15 s frequency for maxillary first and second molars of rats, which were sacrificed immediatelly (group B0), 1 d (group B1), 3 d (group B3), and 7 d (group B7). The irradiation in Group C was changed to 7 W, 20 Hz, and 30 s frequency at 1/2 interval. The expression of 5-HT and CGRP in the pulp tissue of rats maxillary first and second molars was measured by immunohistochemical method. Results: The expressions of CGRP and 5-HT in group B and group C were higher than that of group A. The immediate group began to rise, the 3 day group reached the highest, and then began to decrease. At the same time, group B was higher than group C. Conclusion: The stimulation of semiconductor laser whitening with continuous frequency was significantly higher than that with interval frequency. Laser irradiation immediately increased the expression of pain transmitters 3 days in the pulp, which should strengthen the protection of the pulp.

Key words: Semiconductor laser, Whitening procedure, Pulp, Calcitonin gene-related peptide 5-hydroxytryptamine