甲基丙烯酸羟乙酯对人牙髓组织的毒性及自噬研究

doi:10.13701/j.cnki.kqyxyj.2018.04.012

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (4): 384-387.DOI: 10.13701/j.cnki.kqyxyj.2018.04.012

甲基丙烯酸羟乙酯对人牙髓组织的毒性及自噬研究

朱珅婷, 余静静, 彭彬^*

武汉大学口腔医学院口腔生物医学工程教育部重点实验室湖北武汉 430079

收稿日期:2018-01-04 出版日期:2018-04-28 发布日期:2018-04-25
通讯作者: 彭彬,E-mail:phs301@whu.edu.cn
作者简介:朱珅婷(1992~ ),女,湖北人,博士,主要从事牙体牙髓病学研究。
基金资助:
国家自然科学基金(编号:81300872、81371131)

Inhibition of Human Dental Pulp Viability by 2-Hydroxyethyl Methacrylate.

ZHU Shen-ting, YU Jing-jing, PENG Bin^*.

Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China.

Received:2018-01-04 Online:2018-04-28 Published:2018-04-25

摘要/Abstract

摘要： 目的:探讨HEMA(甲基丙烯酸羟乙酯,2-Hydroxyethyl methacrylate)对牙髓的毒性作用及自噬相关机制。方法:构建新鲜健康年轻恒牙的切片器官培养模型,8 mmol/L HEMA刺激牙切片器官。CCK-8检测HEMA处理12、24、48 h牙切片器官内牙髓组织的活力;苏木精-伊红染色检测48 h后牙髓组织内剩余细胞密度;透射电镜观察不同时间段细胞亚显微结构的变化;组织荧光检测自噬标志分子LC3表达。结果:8 mmol/L HEMA明显抑制牙髓组织活性,并呈现时间依赖性,48 h存活率仅为37.5%。HEMA处理后牙髓组织中细胞亚显微结构改变,并可见明显增加的自噬溶酶体。与对照组相比,HEMA处理组中LC3呈点状聚集,表达明显升高。结论:8 mmol/L HEMA对人牙髓组织有明显的毒性作用,其机制与细胞自噬活化水平的升高有关。

关键词: 甲基丙烯酸羟乙酯, 人牙切片器官, 牙髓组织毒性, 自噬

Abstract: Objective: To investigate the toxicity and possible mechanism of 2-hydroxyethyl methacrylate on human dental pulp in tooth slice organ culture model. Methods: Human tooth slice organ culture model was established in vitro, which was exposed to 8mmol/L HEMA for 12 h, 24 h, and 48 h. Separated dental pulp tissue viability was detected by CCK-8 assessment. Hematoxylin and eosin (H&E) staining demonstrated the cell density change of dental pulp tissue from the ex vivo cultured tooth slice organ model. TEM analysis showed the ultrastructure change of dental mesenchymal cells during autophagy process. Immunostaining detected the LC3 expression in dental pulp. Result: 8 mmol/L HEMA exposure decreased the viability and cell density of dental pulp tissue and increased autophagic vacuoles in a time-dependent manner. And the expression of autophagic biomarkers LC3 increased after 48 h. Conclusion: HEMA greatly decreased the tissue viability and induced autophagy in human dental pulp.

Key words: 2-hydroxyethyl methacrylate, Tooth slice organ, Dental pulp toxicity, Autophagy

朱珅婷, 余静静, 彭彬. 甲基丙烯酸羟乙酯对人牙髓组织的毒性及自噬研究[J]. 口腔医学研究, 2018, 34(4): 384-387.

ZHU Shen-ting, YU Jing-jing, PENG Bin.. Inhibition of Human Dental Pulp Viability by 2-Hydroxyethyl Methacrylate.[J]. Journal of Oral Science Research, 2018, 34(4): 384-387.

参考文献

[1] Williams DW, Wu H, Oh JE, et al. 2-hydroxyethyl methacrylate inhibits migration of dental pulp stem cells [J]. J Endod, 2013, 39(9)∶1156-1160
[2] Krifka S, Spagnuolo G, Schmalz G, et al. A review of adaptive mechanisms in cell responses towards oxidative stress caused by dental resin monomers [J]. Biomaterials, 2013, 34(19)∶4555-4563
[3] Ansteinsson V, Kopperud HB, Morisbak E, et al. Cell toxicity of methacrylate monomers-the role of glutathione adduct formation [J]. J Biomed Mater Res A, 2013,101(12)∶3504-3510
[4] Gallorini M, Cataldi A, Giacomo V. HEMA-induced cytotoxicity: oxidative stress, genotoxicity and apoptosis [J].Int Endod J, 2014, 47(9)∶813-818
[5] 顾中一,费鸿博,李洋洋,等.自噬在骨组织疾病中的调控作用研究进展[J].口腔医学研究, 2017,33(5)∶571-573
[6] McMillan EM, Quadrilatero J. Autophagy is required and protects against apoptosis during myoblast differentiation [J]. Biochem J, 2014, 462(2)∶267-277
[7] Yang Z, Klionsky DJ. Eaten alive: a history of macroautophagy [J]. Nat Cell Biol, 2010, 12(6)∶814-822
[8] Li M, Tan J, Miao Y, et al. The dual role of autophagy under hypoxia-involvement of interaction between autophagy and apoptosis [J]. Apoptosis, 2015, 20(9)∶769-777
[9] Yuan L, Wei SP, Wang J, et al. Isoorientin induces apoptosis and autophagy simultaneously by reactive oxygen species (ROS)-related p53, PI3K/Akt, JNK and p38 signaling pathways in HepG2 cancer cells [J]. J Agric Food Chem, 2014,62(2)∶5390-5400
[10] Yu JJ, Zhu LX, Zhang J, et al. Activation of NF-κB-Autophagy axis by 2-hydroxyethyl methacrylate commits dental mesenchymal cells to apoptosis [J]. Toxicol Sci, 2017,157(1)∶100-111
[11] Gonçalves SB , Dong Z , Bramante CM , et al. Tooth slice-based models for the study of human dental pulp angiogenesis [J]. J Endodontic, 2007, 33, 811-814
[12] Paranjpe A, Bordador LC, Wang MY, et al. Resin monomer 2-hydroxyethyl methacrylate (HEMA) is a potent inducer of apoptotic cell death in human and mouse cells [J]. J Dent Res, 2005,84∶172-177
[13] Liu S, Wu D, Li L,et al. NF-κB activation was involved in reactive oxygen species-mediated apoptosis and autophagy in 1-oxoeudesm-11(13)-eno-12,8α-lactone-treated human lung cancer cells [J]. Arch Pharm Res, 2014, 37(8):1039-1052
[14] Nikoletopoulou V, Markaki M, Palikaras, K, et al. Crosstalk between apoptosis, necrosis and autophagy [J]. Biochim Biophys Acta, 2013, 1833(12)∶3448-3459
[15] Marino G, Niso-Santano M, Baehrecke EH, et al. Self-consumption: the interplay of autophagy and apoptosis [J]. Nat Rev Mol Cell Biol, 2014,15(3)∶81-94

甲基丙烯酸羟乙酯对人牙髓组织的毒性及自噬研究

Inhibition of Human Dental Pulp Viability by 2-Hydroxyethyl Methacrylate.

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 14

编辑推荐

Metrics

[1]	齐晓爽, 孟维艳. 用于牙周炎治疗的自噬调节物研究现状[J]. 口腔医学研究, 2020, 36(7): 616-618.
[2]	黄佩, 乔玮玮, 孟柳燕. 自噬通过GATA4调控人牙髓细胞衰老反应[J]. 口腔医学研究, 2020, 36(12): 1142-1147.
[3]	毛家奇, 黄蔚, 於丽明, 赵力如, 杨冬茹, 卢芸. 低氧对C2C12细胞分化不同阶段自噬和肌细胞生成素表达的影响[J]. 口腔医学研究, 2020, 36(1): 70-74.
[4]	陈杰,徐华兴,程抒华,张旗. 细胞自噬在牙髓损伤修复过程中的作用[J]. 口腔医学研究, 2019, 35(6): 531-536.
[5]	孙维克, 王培源, 刘伟, 王霞. Beclin1与凋亡相关蛋白在口腔鳞癌中的表达及意义[J]. 口腔医学研究, 2019, 35(10): 979-983.
[6]	刘建国, 敬珮. 内质网应激与自噬在口腔疾病研究中的应用[J]. 口腔医学研究, 2018, 34(5): 461-466.
[7]	刘玉兰, 孟琳, 唐琪, 布文奂, 黄国帅, 戴义宾, 刘麒麟, 孙宏晨. 抗坏血酸碳点通过促进自噬杀伤舌鳞癌细胞[J]. 口腔医学研究, 2018, 34(1): 35-38.
[8]	章思莹，潘淑婷，帅芳源，邱嘉旋. PI3K/AKT/mTOR/p70S6K通路在人舌鳞癌细胞耐药中的作用[J]. 口腔医学研究, 2017, 33(9): 938-942.
[9]	邵江红, 陈芳, 孔宇. miR-30a在雷帕霉素刺激人炎症牙周膜干细胞后的表达情况及其与Beclin1基因的靶向调控作用的研究[J]. 口腔医学研究, 2017, 33(6): 658-662.
[10]	顾中一,费鸿博,李洋洋,徐晓薇,孙宏晨,林崇韬. 自噬在骨组织疾病中的调控作用研究进展[J]. 口腔医学研究, 2017, 33(5): 571-573.
[11]	曾光,卫克文. MAP4K3-mTOR信号在甘草查而酮A诱导的鳞癌细胞自噬与凋亡中的作用研究[J]. 口腔医学研究, 2017, 33(12): 1241-1245.
[12]	王一博, 孙宾, 王丹丹, 唐琪, 孙宏晨. 纳米材料诱导的自噬与凋亡在抑制肿瘤生长和治疗中的作用及研究进展[J]. 口腔医学研究, 2017, 33(11): 1230-1233.
[13]	王倩, 马金宝, 白桦. 自噬相关蛋白LC3Av1在口腔鳞状细胞癌中表达及甲基化对其的影响[J]. 口腔医学研究, 2015, 31(4): 373-376.
[14]	江丽斌, 秦文, 刘文佳, 邵金陵, 郭涛. 低氧环境下自噬对颏舌肌卫星细胞增殖与凋亡的影响[J]. 口腔医学研究, 2015, 31(3): 209-212.