纳米材料诱导的自噬与凋亡在抑制肿瘤生长和治疗中的作用及研究进展

doi:10.13701/j.cnki.kqyxyj.2017.11.025

摘要/Abstract

摘要： 细胞自噬是细胞自身调节的重要生理反应,它与纳米颗粒有着密切的关联,近年来纳米与细胞自噬的研究成为生物医学领域的研究热点,有研究表明通过纳米颗粒有效的诱导在特定细胞中的自噬,可以加速病变细胞的死亡,对于治疗肿瘤和神经系统疾病等有着重要意义。本文介绍了细胞的自噬与凋亡过程、自噬与凋亡相互作用机制,阐述了不同类别的纳米颗粒诱导的自噬效应,并对纳米材料诱导自噬应用进行了展望,指出在研究中重点加强纳米颗粒与细胞自噬作用机理研究和纳米颗粒的可控性研究,更好地应用于生命科学。

关键词: 自噬, 凋亡, 纳米颗粒, 毒性

Abstract: Cell autophagy is an important physiological response of cell self-regulation. It is closely related to nanoparticles. In recent years, nanoparticle and autophagy have become a hotspot in biomedical research. The results show that the effective induction of autophagy by nanoparticles in the specific cells can accelerate the death of diseased cells, which has important significance for the treatment of tumors and nervous system diseases. In this paper, the progress of autophagy and apoptosis and the mechanism of autophagy and apoptosis were introduced. The autophagy induced by different nanoparticles was discussed. The application of nanomaterials in autophagy was also discussed. It is our duty to focus on the study of mechanism of nanoparticle and cell autophagy and the controllability of nanoparticles so as to better apply to life science and help to cure diseases.

Key words: Autophagy, Apoptosis, Nanoparticles, Toxicity

中图分类号:

R780.2

王一博, 孙宾, 王丹丹, 唐琪, 孙宏晨. 纳米材料诱导的自噬与凋亡在抑制肿瘤生长和治疗中的作用及研究进展[J]. 口腔医学研究, 2017, 33(11): 1230-1233.

WANG Yi-bo, SUN Bing, WANG Dan-dan, TANG Qi, SUN Hong-chen. Effect and Progress of Autophagy Induced by Nanoparticles on Inhibition of Tumor Growth and Treatment[J]. Journal of Oral Science Research, 2017, 33(11): 1230-1233.

参考文献

[1] Mizushima N. Autophagy process and function [J]. Genes Dev, 2007, 21(22)∶2861-2873
[2] Levine B , and Kroemer G. Autophagy in the pathogenesis of disease [J]. Cell, 2008, 132(1)∶27-42
[3] Sinha S , and Levine B. The autophagy effector Beclin 1: a novel BH3-only protein [J]. Oncogene , 2008, 27(1)∶137-148
[4] Mathew R , Karantza-Wadsworth V , and White E. Role of autophagy in cancer [J]. Nat. Rev. Cancer , 2008, 7(12)∶961-967
[5] Maiuri M C, Zalckvar E, Kimchi A, et al. Self-eating and self-killing: crosstalk between autophagy and apoptosis [J]. Nature Reviews Molecular Cell Biology, 2007, 8(9)∶741
[6] 白宇,全海英,张桐菲,等.顺铂诱导的自噬在口腔鳞癌Tca83细胞中的作用[J].口腔医学研究,2012,28(6)∶566-569
[7] 江丽斌,秦文,刘文佳,等.低氧环境下自噬对颏舌肌卫星细胞增殖与凋亡的影响[J].口腔医学研究,2015,31(3)∶209-212
[8] Guan J L, Simon A K, Prescott M, et al. Autophagy in stem cells [J]. Autophagy, 2013, 9(6)∶830-849
[9] Bernard S, Eilers M. Control of cell proliferation and growth by Myc proteins [J]. Results Probl Cell Differ, 2006, 42∶329-342
[10] Maiuri M C, Toumelin G L, Criollo A, et al. Functional and physical interaction between Bcl-X(L) and a BH3-like domain in Beclin-1 [J]. Embo Journal, 2007, 26(26)∶2527-2539
[11] Oberstein A, Jeffrey P D, Shi Y. Crystal structure of the Bcl-XL-Beclin 1 peptide complex: Beclin 1 is a novel BH3-only protein [J]. Journal of Biological Chemistry, 2007, 282(17)∶13123
[12] Danial N N, Korsmeyer S J. Cell death: critical control points [J]. Cell, 2004, 116(2)∶205
[13] Degenhardt K, Chen G, Lindsten T, et al. BAX and BAK mediate p53-independent suppression of tumorigenesis [J]. Cancer Cell, 2002, 2(3)∶193-203
[14] Yue Z, Jin S, Yang C, et al. Beclin 1, an autophagy gene essential for early embryonic development, is a haploinsufficient tumor suppressor [J]. Proceedings of the National Academy of Sciences, 2004, 100(25)∶15077-15082
[15] White E, and DiPaola RS. The double-edged sword of autophagy modulation in cancer [J]. Clin. Cancer Res, 2009, 15(17)∶5308-5316
[16] Apel A, Zentgraf H, Buchler MW, et al. Autophagy-A double edged sword in oncology [J]. Int. J. Cancer, 2009, 125(5)∶991-995
[17] Lu Z , Luo RZ , Lu Y , et al. The tumor suppressor gene ARHI regulates autophagyand tumor dormancy in human ovarian cancer cells [J]. J. Clin. Invest, 2008, 118(12)∶3917-3929
[18] Seleverstov O, Zabirnyk O, Zscharnack M, et al. Quantum dots for human mesenchymal stem cells labeling. A size-dependent autophagy activation [J]. Nano Lett, 2007, 6(12)∶2826-2832
[19] Stern ST, Zolnik BS, McLeland CB, et al. Induction of autophagy in porcine kidney cells by quantum dots: A common cellular response to nanomaterials [J]. Toxicol Sci, 2008, 106(1)∶140-152
[20] Mishra A, Zheng J, Tang X, et al.Silver Nanoparticle-Induced Autophagic -Lysosomal Disruption and NLRP3-Inflammasome Activation in HepG2 Cells is Size-Dependent [J]. Toxicological Sciences, 2016, 150(2)∶kfw011
[21] Zhang Q, Yang WJ, Man N, et al. Autophagy-mediated chemosensitization in cancer cells by fullerene C60 nanocrystal [J]. Autophagy, 2009,5(8)∶1107-1117
[22] Wei PF, Zhang L, Lu Y, et al. C60(Nd) nanoparticles enhance chemotherapeutic susceptibility of cancer cells by modulation of autophagy [J]. Nanotechnology, 2010, 21(49)∶495101
[23] 崔之芬,诸颖,李晓明,等.纳米颗粒诱导的自噬效应及在生物医药领域的应用[J].科学通报,2013,58(34)∶3521-3529
[24] Chen GY, Yang HJ, Lu CH, et al. Simultaneous induction of autophagy and toll-like receptor signaling pathways by graphene oxide [J]. Biomaterials, 2012, 33(27)∶6559-6569
[25] Markovic ZM, Ristic BZ, Arsikin KM, et al. Graphene quantum dots as autophagy-inducing photodynamic agents [J]. Biomaterials, 2012, 33(29)∶7084-7092
[26] Fude W, Chan J, Hao L, et al. Effects of fullerene C?? nanoparticles on A549 cells [J]. Environmental Toxicology & Pharmacology, 2014, 37(2)∶656-61
[27] Li JJ, Hartono D, Ong CN, et al. Autophagy and oxidative stress associated with gold nanoparticles [J]. Biomaterials, 2010, 31(23)∶5996-6003
[28] Panzarini E, Mariano S, Dini L. Glycans coated silver nanoparticles induces autophagy and necrosis in HeLa cells [C]. Nanoforum, 2015, 1667(1)∶020017
[29] Farah MA, Ali MA, Chen SM, et al. Silver nanoparticles synthesized from Adenium obesum leaf extract induced DNA damage, apoptosis and autophagy via generation of reactive oxygen species [J]. Colloids & Surfaces B Biointerfaces, 2016, 141∶158
[30] Zhang YJ, Zheng F, Yang TL, et al. Tuning the autophagy-inducing activity of lanthanide-based nanocrystals through specific surface-coating peptides [J]. Nat Mater, 2012, 11(9)∶817-826
[31] Khan MI, Mohammad A, Patil G, et al. Induction of ROS, mitochondrial damage and autophagy in lung epithelial cancer cells by iron oxide nanoparticles [J]. Biomaterials, 2012, 33(5)∶1477-1488
[32] Sun TT, Yan YW, Zhao Y, et al. Copper oxide nanoparticles induce autophagic cell death in A549 cells [J]. PLoS One, 2012, 7(8)∶e43442
[33] Li HY, Li YH, Jiao J, et al. Alpha-alumina nanoparticles induce efficient autophagy-dependent cross-presentation and potent antitumour response [J]. Nat Nanotech, 2011, 6(10)∶645-650
[34] Lu Y, Zhang L, Li J, et al. MnO nanocrystals: A platform for integration of MRI and genuine autophagy induction for chemotherapy [J]. AdvFunct Mater, 2013(12), 23∶1534-1546
[35] Yu KN, Yoon TJ, Tehrani AM, et al. Zinc oxide nanoparticle induced autophagic cell death and mitochondrial damage via reactive oxygen species generation [J]. Toxicol in Vitro, 2013, 27(4)∶1187-1195
[36] Chen Y, Yang LS, Feng C, et al. Nano neodymium oxide induces massive vacuolization and autophagic cell death in non-small cell lung cancer NCI-H460 cells [J]. Biochem Biophys Res Commun, 2005, 337(1)∶52-60
[37] Yu L, Lu Y, Man N, et al. Rare earth oxide nanocrystals induce autophagy in HeLa cells [J]. Small, 2009, 5(24)∶2784-2787
[38] Zhang Y, Yu CG, Huang GY, et al. Nano rare earth oxides induced size dependent vacuolization, an independent pathway from autophagy [J]. Int J Nanomed, 2010, 5(24)∶601-609
[39] Man N, Yu L, Yu SH, et al. Rare earth oxide nanocrystals as a new class of autophagy inducers [J]. Autophagy, 2010, 6(2)∶310-311