口腔医学研究

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (5): 457-460.

• 基础研究论著 • 上一篇    下一篇

3种义齿粘附剂的体外细胞毒性研究

陈凤英, 程祥荣*   

  1. 武汉大学口腔医学院口腔生物医学工程教育部重点实验室 湖北 武汉 430079
  • 收稿日期:2014-09-17 出版日期:2015-05-28 发布日期:2016-04-29
  • 通讯作者: 程祥荣,电话:027-87686221
  • 作者简介:陈凤英(1974~ ),女,湖北襄阳人,博士,主治医师,主要从事口腔修复学临床及基础研究工作。

In Vitro Studies on the Cytotoxicity of Three Commercial Denture Adhesives.

CHEN Feng-ying, CHENG Xiang-rong.   

  1. Key Lab for Oral Biomedical Engineering of Ministry of Education, School of Stomatology, Wuhan University, Wuhan 430079
  • Received:2014-09-17 Online:2015-05-28 Published:2016-04-29

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摘要： 目的:体外研究3种商品化义齿粘附剂的细胞毒性。方法:采用MTT法检测不同浓度义齿粘附剂浸提液对人原代口腔黏膜角质细胞、成纤维细胞和小鼠成纤维细胞系L929的细胞毒性。结果:3种义齿粘附剂对人原代口腔黏膜角质细胞有1~2级细胞毒性,对人原代口腔黏膜成纤维细胞有1级细胞毒性,对L929细胞却无毒性。高浓度义齿粘附剂浸提液一般比低浓度浸提液具有更强的细胞生长抑制作用。结论:义齿粘附剂对人原代口腔黏膜角质细胞和成纤维细胞有细胞毒性,对L929细胞却无细胞毒性,提示在义齿粘附剂的细胞毒性测试方面,人原代口腔黏膜细胞可能会提供更有价值的信息。

关键词: 义齿粘附剂, 人原代口腔黏膜细胞, MTT法, 细胞毒性

Abstract: Objective: To investigate the cytotoxicity of three commercial denture adhesives. Methods: The cytotoxic effects of three denture adhesive eluates with different concentrations on primary human oral keratinocytes(HOKs), fibroblasts (HOFs) and permanent mouse fibroblasts cell lines (L929) were tested using MTT assay. Results: Three tested denture adhesives showed cytotoxicity to primary HOKs(levels 1 to 2) and HOFs( level 1), whereas none of three was toxic to L929 cells. Higher concentrations of adhesive eluates generally showed significantly stronger inhibitory effects than the lower ones. Conclusion: Denture adhesives are toxic to the primary HOKs and HOFs cultures, whereas non-toxic to L929 cells. The results suggest that primary human oral mucosal cells may provide more valuable information in toxicity screening of denture adhesives.

Key words: Denture adhesive , Primary human oral mucosal cells , MTT assay , Cytotoxicity

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