口腔医学研究

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (5): 427-431.DOI: 10.13701/j.cnki.kqyxyj.2016.05.001

• 基础研究论著 •    下一篇

PGS/PLLA共纺膜片的降解性能及生物相容性研究

钟妮1 ,包广洁2 ,李燕梅1 ,吕玮1 ,张文霞1 ,赵红斌3 ,康宏1*   

  1. 1. 兰州大学 口腔医学院口腔修复科 甘肃 兰州 730000;
    2. 西北民族大学 口腔医学国家民委重点实验室 甘肃 兰州 730030;
    3. 解放军兰州军区兰州总医院 甘肃 兰州 730050
  • 收稿日期:2015-10-23 出版日期:2016-05-26 发布日期:2016-05-26
  • 通讯作者: 康宏,E-mail:kanghong@lzu.edu.cn
  • 作者简介:钟妮(1989~ ),女,陕西人,硕士在读,主要从事颞下颌关节盘组织工程的研究。
  • 基金资助:
    国家自然科学基金资助项目(编号:81160139)

Study on Degradation and Biological Compatibility of PGS/PLLA Electrospuning Films.

ZHONG Ni1,BAO Guangjie2, LI Yan-mei1, LV Wei1, ZHANG Wen-xia1, ZHAO Hong-bin3,KANG Hong1*.   

  1. 1. School of Stomatology, Lanzhou University, Lanzhou 730000, China;
    2. Key Lab of Stomatology of State Ethnic Affairs Commission, Northwest University for Nationalities, Lanzhou 730030, China;
    3. Lanzhou General Hospital of Lanzhou Military Command of the PLA, Lanzhou 730050, China
  • Received:2015-10-23 Online:2016-05-26 Published:2016-05-26

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摘要： 目的:观察PGS/PLLA共纺膜片体外降解性及细胞相容性,探究其作为山羊颞下颌关节盘组织工程支架的可行性,为进一步动物实验奠定基础。方法:将PGS/PLLA共纺膜片浸泡在磷酸盐缓冲液中(pH=7.4),不同时间点取样,对材料微观形貌、pH、力学性能等进行检测;将关节盘细胞-PGS/PLLA支架复合培养,MTT法检测支架的细胞毒性及对关节盘细胞增殖影响;在14 d时,取出关节盘细胞-PGS/PLLA复合物,行HE、番红O、免疫组化染色。结果:PGS/PLLA支架降解性能适宜;MTT结果示,支架无毒性,细胞增殖良好。结论:颞下颌关节盘细胞在支架上粘附良好、增殖明显、代谢活跃,PGS/PLLA支架有望作为颞下颌关节盘组织工程支架。

关键词: PGS/PLLA, 降解性, 细胞相容性

Abstract: Objective: To explore the feasibility of the poly-glycerol-sebacate/poly-l-lactic acid (PGS/PLLA) electrospuning film as a scaffold of goat TMJ disc tissue engineering by assessing the degradation and cytocompatibility in vitro. Methods: The morphological features, weight loss, pH value changes in the PBS, and the mechanical properties of the PGS/PLLA electrospuning films were evaluated during the degradation process. Moreover, the TMJ disc cells were co-cultured with the PGS/PLLA scaffold and the biocompatibility was assessed by MTT assays. Finally, the hematoxylin-eosin staining, safranin O staining and immunohistochemistry staining were tested on the 14th day. Results: The PGS/PLLA electrospuning films showed proper degradation performance. Nontoxic properties and favorable cell proliferation were observed. Conclusion: TMJ disc cells can adhesive and proliferate on the PGS/PLLA scaffold. The PGS/PLLA can serve as a potential scaffold to repair the TMJ disc disease.

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