口腔医学研究

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (10): 1001-1004.DOI: 10.13701/j.cnki.kqyxyj.2019.10.021

• 其他研究 • 上一篇    下一篇

镁合金微弧氧化掺杂Zn-HA复合涂层细胞相容性与抗菌性

张鹏1, 周立波1, 张慧明2, 张美玲1, 姜枫1, 黄宇博1, 李慕勤3*, 李德超1*   

  1. 1. 佳木斯大学附属口腔医院 黑龙江 佳木斯 154007;
    2. 佳木斯大学生命科学中心 黑龙江 佳木斯 154007;
    3. 黑龙江省高校生物医学材料重点实验室 黑龙江 佳木斯 154007
  • 收稿日期:2019-03-04 出版日期:2019-10-28 发布日期:2019-10-22
  • 通讯作者: 李慕勤,E-mail: jmsdxlimuqin@163.com;李德超,E-mail: dechaoli2004@163.com
  • 作者简介:张鹏(1987~ ),男,黑龙江鹤岗人,硕士,研究方向:口腔种植医学。
  • 基金资助:
    国家自然科学基金(编号:31370979)黑龙江省卫生计生委科研课题(编号:2018037)佳木斯大学口腔病防治协同创新中心(编号:2011xtcx2016-02)

Cellular Compatibility and Antibacterial Properties of Magnesium Alloy after Ultrasonic Microarc Oxidation-doped Zn-HA Silane-phytic Acid Treatment.

ZHANG Peng1, ZHOU Libo1, ZHANG Huiming2, ZHANG Meiling1, JIANG Feng1, HUANG Yubo1, LI Muqin3*, LI Dechao1*   

  1. 1. Stomatological Hospital, Jiamusi University, Jiamusi 154007, China;
    2. Life Science Center, Jiamusi University, Jiamusi 154007, China;
    3. Key Laboratory of College of Biomedical Materials of Heilongjiang Province, Jiamusi University, Jiamusi 154007, China.
  • Received:2019-03-04 Online:2019-10-28 Published:2019-10-22

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摘要： 目的:通过掺杂Zn-HA复合涂层处理调节镁合金的生物活性和增加抗菌性。方法:以镁合金微弧氧化硅烷-植酸复合膜为对照组,添加2 g/L、4 g/L、6 g/L Zn-HA为实验组,通过扫描电镜、CCK-8试剂盒、激光共聚焦显微镜等方式检测各组膜层生物相容性,采用吖啶橙/溴化乙锭(AO/EB)双重荧光染色法检测抗菌性。结果:CCK-8粘附与增殖实验测定的吸光率结果均为C组＞D组＞B组＞A组;激光共聚焦显微镜显示载4 g/L Zn-HA组试件表面细胞伸展最好,载6 g/L Zn-HA后涂层的抗菌性优于其他各组。结论:镁合金微弧氧化掺杂4 g/L Zn-HA硅烷-植酸处理后膜层的生物相容性最好,镁合金微弧氧化掺杂6 g/L Zn-HA硅烷-植酸处理后膜层具有较强抗菌能力。

关键词: 镁合金, 超声微弧氧化, Zn-HA, 硅烷, 植酸, 细胞相容性, 抗菌性

Abstract: Objective: To test the cellular biocompatibility and antibacterial property of magnesium alloy after ultrasonic microarc oxidation-doped Zn-HA silane-phytic acid treatment. Methods: The magnesium alloy UMAO-silane-phytic was designed as the control group. Zn-HA at concentration of 2 g/L, 4 g/L, and 6 g/L were added respectively as the experimental group. The compatibility of different treatment membrane cells was detected by CCK-8 kit, scanning electron microscope, and laser confocal microscope. Acridine orange/ethidium bromide (AO/EB) double fluorescent staining was used to detect the antibacterial. Results: The optical density (OD) value of cell adhesion and proliferation measured by CCK-8. The OD values were in the order at: group C>group D>group B>group A. The　number and shape of cells in the group C had the best extension. The antibacterial of 6 g/L Zn-HA group was obviously superior to other groups. Conclusion: The UMAO-doped 4 g/L Zn-HA silane-phytic acid treatment of magnesium alloy had the best biocompatibility. The UMAO-doped 6g/L Zn-HA silane-phytic acid treatment of magnesium alloy had the strongest antibacterial ability.

Key words: magnesium alloy, ultrasonic microarc oxidation, Zn-HA, silane phytic acid, cellular compatibility, antibacterial, properties