口腔医学研究 ›› 2018, Vol. 34 ›› Issue (7): 793-797.DOI: 10.13701/j.cnki.kqyxyj.2018.07.026

• 其他研究 • 上一篇    下一篇

纯镁阳极氧化膜层不同后处理的细胞相容性

刘继光, 李翀乾, 张慧明, 李慕勤*, 沈柏芳, 林叶   

  1. 佳木斯大学 黑龙江省高校生物医学材料重点实验室 黑龙江 佳木斯 154007
  • 收稿日期:2018-01-04 出版日期:2018-07-28 发布日期:2018-07-20
  • 通讯作者: 李慕勤,E-mail:jmsdxlimuqin@163.com
  • 作者简介:刘继光(1961~ ),男,黑龙江省绥化人,博士,教授,主要从事生物医学材料方面的研究。
  • 基金资助:
    国家自然科学基金(编号:31370979)

Cytocompatibility of Pure Magnesium Anodic Oxidation Film after Different Post-treatments

LIU Ji-guang, LI Chong-qian, ZHANG Hui-ming, LI Mu-qin*, SHEN Bai-fang, LIN Ye   

  1. Key Laboratory of Biomedical Materials of Colleges and Universities in Heilongjiang, Jiamusi University, Jiamusi 154007, China.
  • Received:2018-01-04 Online:2018-07-28 Published:2018-07-20

摘要: 目的:通过对纯镁阳极氧化膜层表面进行不同后处理,期望提高复合膜层的耐腐蚀性及细胞相容性。方法:实验分为纯镁阳极氧化处理(A组)、纯镁阳极氧化-硅烷处理(B组)、纯镁阳极氧化-硅烷-植酸处理(C组)、纯镁阳极氧化-硅烷-植酸载铜处理(D组)。采用扫描电镜法研究膜层形貌,激光共聚焦法观察细胞骨架及铺展情况,CCK-8法检测不同膜层表面的细胞粘附、增殖能力。结果: 通过对纯镁阳极氧化膜层表面进行硅烷、植酸、载铜处理,有效地减少了其表面的孔隙,其中D组﹥C组﹥B组﹥A组;各膜层表面细胞铺展、粘附、增殖情况皆为C组﹥D组﹥B组﹥A组。结论: 纯镁阳极氧化-硅烷-植酸载铜膜层封孔最好,细胞活性较好,硅烷-植酸处理组膜层细胞生物活性最佳。

关键词: 纯镁, 阳极氧化, 硅烷, 植酸,

Abstract: Objective: To test the corrosion resistance and cell compatibility of pure magnesium anodic oxidation film after different treatments. Methods: The experiments were divided into pure magnesium anodic oxidation group (group A), pure magnesium anodic oxidation-silane treatment group (group B), pure magnesium anodic oxidation-silane-phytic acid treatment group (group C), pure magnesium anodic oxidation-silane-phytic acid coated copper treatment group (group D). Scanning electron microscopy, laser confocal microscopy, and CCK-8 were used to study the morphology of the films, the cytoskeleton and spreading, and the cell adhesion and proliferation ability of different membranes. Results: The pores of the surface of pure magnesium anodized film were effectively reduced by silane, phytic acid and copper loading, among which, group D>group C>group B>group A. The results of the film surface spreading, adhesion, proliferation of four groups were group C>group D>group B>group A. Conclusion: Pure magnesium anodic oxidation-silane-phytic acid coated copper film has the best sealing ability and good cell activity, and the silane-phytic acid treatment group has the best biological activity.

Key words: Pure magnesium, Anodic oxidation, Silane, Phytic acid, Copper