口腔医学研究

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (4): 374-378.DOI: 10.13701/j.cnki.kqyxyj.2016.04.014

• 临床研究论著 • 上一篇    下一篇

植酸作为螯合剂去除根管玷污层的效力及其对牙本质化学组成的影响

王天丰1,曹茜茜1,撒悦1,2,蒋滔1,2*   

  1. 1. 武汉大学口腔医学院口腔基础医学省部共建国家重点实验室培训基地和口腔生物医学教育部重点实验室 湖北 武汉 430079;
    2. 武汉大学口腔医院修复科 湖北 武汉 430079
  • 收稿日期:2016-01-19 出版日期:2016-04-28 发布日期:2016-04-28
  • 通讯作者: 蒋滔,电话:027-87686318
  • 作者简介:王天丰(1989~ ),女,湖北武汉人,硕士在读,主要从事牙齿结构,生物矿化研究工作。
  • 基金资助:
    国家自然科学基金项目(编号:81500887,81470771)中央高校基本科研业务费专项基金项目(编号:2042015kf0093)

Effect of Phytic Acid Used as Chelating Agent on Smear Layer and Chemical Composition of Dentin.

WANG Tian-feng1, CAO Xi-xi1, SA Yue1,2, JIANG Tao1,2*.   

  1. 1. The State Key Laboratory Breeding Base of Basic Science of Stomatology &Key Laboratory of Oral Biomedicine Ministry of Education, School of Stomatology, Wuhan University, Wuhan 430079, China;
    2. Department of Prosthodontics, School of Stomatology, Wuhan University, Wuhan 430079, China
  • Received:2016-01-19 Online:2016-04-28 Published:2016-04-28

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3

摘要： 目的:通过体外实验评价植酸(IP6)作为根管螯合剂去除次氯酸钠溶液处理的牙本质表面玷污层的效力,探究其对次氯酸钠溶液处理的牙本质化学组成的影响。方法:24个完整无龋正畸牙取得牙本质样本随机分成4组。A组:2 mL去离子水(pH7.4)处理5 min;B组:2 mL 5%NaClO溶液(pH12)处理5 min;C组:2 mL 5%NaClO溶液处理5 min后,2 mL 17% EDTA溶液(pH7.5)处理1 min;D组:2 mL 5% NaClO溶液处理5 min后,2 mL 1% IP6溶液(pH1.3)处理1 min。SEM观察样本表面玷污层去除情况,衰减全反射红外光谱仪(ATR-IR)测量样本红外光谱,得到处理前后的M:M(matrix:mineral)和C:M(carbonate:mineral)。以双因素重复测量方差分析比较差异。结果:SEM显示处理后A组仍有大量玷污层剩余,B组玷污层去除效果不明显,C组玷污层基本完全去除,D组玷污去除效果更加明显。ATR-IR结果经统计分析后发现,A组M:M处理前后无显著差异(P=0.744),B、C、D组均显著下降(P<0.01);处理后B、C、D组明显低于A组(P<0.01),而该3组间无显著差异(P>0.05);四组C:M处理后均略低于处理前(P<0.01)。结论:植酸能够有效地去除经次氯酸钠溶液处理后的牙本质表面的玷污层,并且其与EDTA对牙本质本身化学组成的影响无显著差别。

关键词: 植酸, 牙本质, 玷污层, 扫描电镜, 红外光谱

Abstract: Objective: To investigate the effect of phytic acid (inositol hexakisphosphate,IP6), as a root canal chelating agent on the smear layer and the chemical composition of dentin treated with sodium hypochlorite (NaClO). Methods: Twenty-four intact dentin specimens from the crown of premolars were randomly assigned into four groups (n=6) and treated with 2 mL deionized water (5 min), 2mL 5% NaClO (5 min), 2mL 5% NaClO (5 min) followed by 2mL 17% EDTA or 1% IP6 (1 min). The presence of smear layer was evaluated with scanning electron microscopy (SEM). The dentinal infrared spectrum was recorded by attenuated total reflection infrared(ATR-IR) spectrometer. M:M(matrix:mineral)and C:M(carbonated:mineral) were calculated and analyzed by two-way RMANOVA. Results: The SEM images showed that 5% NaClO followed by 17% EDTA or 1% IP6 almost removed the smear layer completely, while DW and 5% NaClO alone left a large number of smear layer. It revealed that M:M remained unchanged in group DW (p=0.744), but it decreased significantly in group 5% NaClO alone or followed by 17% EDTA or 1% IP6 (P<0.01). M:M of the latter three groups was obviously lower than that of group DW (P<0.01), and there was no significant difference among the three groups (P>0.05). C:M decreased

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