口腔医学研究

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (4): 379-382.DOI: 10.13701/j.cnki.kqyxyj.2016.04.015

• 临床研究论著 • 上一篇    下一篇

慢性牙周炎患者龈沟液及牙龈组织中Dickkopf-l水平

金晶,吴凯悦#,许春姣*,池毓坦,孙晓娟,袁海波,邱喜丽,王宏峰,李冬梅   

  1. 中南大学湘雅医院口腔医学中心 湖南 长沙 410008
  • 收稿日期:2015-12-16 出版日期:2016-04-28 发布日期:2016-04-28
  • 通讯作者: 许春姣,电话:0731-84327495
  • 作者简介:金晶(1986~ ),女,舟山人,硕士,主要从事牙周黏膜病的研究工作。
    吴凯悦(1990~ ),女,北京人,硕士,主要从事牙周黏膜病的研究工作。
  • 基金资助:
    湖南省发改委高新技术计划项目(湘发改高技【2012】1493号)教育部留学回国人员科研启动基金课题(教外司留【2012】940号)

Levels of Dickkopf-l in Gingival Crevicular Fluid and Gingival Tissue from Chronic Periodontitis.

JIN Jing, WU Kai-yue#, XU Chun-jiao*, CHI Yu-tan, SUN Xiao-juan, YUAN Hai-bo, QIU Xi-li, WANG Hong-feng, LI Dong-mei   

  1. The Center of Stomatology of Xiangya Hospital, Central South University, Changsha 410008, China
  • Received:2015-12-16 Online:2016-04-28 Published:2016-04-28

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摘要： 目的:检测Dkk1(Dickkopf-l)在慢性牙周炎(chronic periodontitis,CP)患者龈沟液及牙龈组织中的表达,探讨DKK1在CP发生发展过程中的作用。方法:选择CP患者30例为试验组,另外选择牙周健康者10例为对照组。收集两组研究对象的龈沟液(gingival crevicular fluid,GCF),用酶联免疫吸附法测定GCF中Dkk1的质量浓度。结果:CP组GCF中Dkk1质量浓度(8.86±4.07) μg/L明显高于对照组(5.5±2.05) μg/L(P<0.05)。轻(5.17±1.49) μg/L、中(8.32±1.25) μg/L、重(13.07±1.80) μg/L度CP组的DKK1浓度呈递增趋势(P<0.05),轻度CP组与对照组之间DKK1浓度无明显差异。CP组GCF中Dkk1浓度与牙周袋深度(PD)、临床附着丧失(CAL)呈正相关(r=0.769、0.802,P<0.05)。(2)CP组、牙龈炎组及对照组牙龈组织Dkk1平均光密度分别为(13.40±2.83)×10-2、(7.95±4.52)×10-2、(5.31±1.40)×10-2,CP组显著高于牙龈炎组、对照组(P＜0.05);后两组之间差异无统计学意义。结论:Dkk1参与慢性牙周炎的发生、发展。

关键词: 慢性牙周炎, Dkk1, 免疫组织化学, 酶联免疫吸附实验

Abstract: Objective:To investigate the levels of Dkk1 in chronic periodontitis (CP), and explore the relationship between DKK1 and the occurrence and development of CP. Methods:Thirty CP patients were sub-divided into mild, moderate, and severe CP groups according to clinical attachment loss (CAL) and alveolar bone resorption data. Ten healthy volunteers were recruited as the control group. Gingival crevicular fluid (GCF) was collected from all people and the concentration of DKK1 in the GCF samples was detected using enzyme-linked immunosorbent assay (ELISA). Results: The concentration of Dkk1 in GCF of the CP groups (8.86±4.07)μg/L was significantly higher than that in the control group (5.51±2.05)μg/L (P<0.05). The concentration of DKK1 in the mild (5.17±1.49)μg/L, moderate (8.32±1.25)μg/L, and severe (13.07±1.80)μg/L CP groups increased significantly (P<0.05). There was a significant positive correlation between the concentrations of Dkk1 in GCF and PD, CAL. The mean optical density of Dkk1 in the gingival tissues of CP group was (13.40±2.83)×10-2, in the gingivitis group was (7.95±4.52)×10-2, in the control group was (5.31±1.40)×10-2. The mean optical density of Dkk1 in the CP group was significantly higher than that in the gingivitis group and control group (P<0.05). The difference between the latter two groups was not statistically significant. Conclusion: Dkk1 may play a role in the development of periodontitis

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