口腔医学研究

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (12): 1225-1228.DOI: 10.13701/j.cnki.kqyxyj.2016.12.001

• 名家论道 •    下一篇

两种沟槽钛片上纤维连接蛋白修饰方法的效果比较

黄斯佳1,许志强2,赖颖真3,周麟4,陈江4*   

  1. 1. 泉州医学高等专科学校 福建 泉州 362000;
    2. 莆田学院附属医院口腔科 福建 莆田 351100;
    3. 厦门医学高等专科学校 福建 厦门 361000;
    4. 福建医科大学附属口腔医院 福建 福州 350004
  • 收稿日期:2016-07-21 出版日期:2016-12-25 发布日期:2016-12-26
  • 通讯作者: 陈江,电话:0595—22779291
  • 作者简介:黄斯佳(1983~ ),男,福建泉州人,硕士,主治医师,主要从事口腔种植的临床治疗工作。
  • 基金资助:
    福建省自然科学基金项目(编号:2013J05112)

Comparison of Two Methods to Modify Groove Titanium Plate with Fibronectin.

HUANG Si-Jia1, XU Zhi-Qiang2, LAI Ying-Zhen3, ZHOU Lin4, CHEN Jiang4*.   

  1. 1. Quan zhou Medical College, Quanzhou 362000, China;
    2. Department of Stomatology, Affiliated Hospital of Putian University, Putian 351100, China;
    3. Xiamen Medical College, Xiamen 361000, China;
    4. Stomatological Hospital, Fujian Medical University, Fuzhou 350004, China.
  • Received:2016-07-21 Online:2016-12-25 Published:2016-12-26

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摘要： 目的:比较三氟代乙烷磺酰氯与硅烷化两种钛片上修饰纤维粘结蛋白(Fibronectin,Fn)方法的效果。方法:通过三氟代乙烷磺酰氯与硅烷化将Fn修饰于微沟槽钛表面,XPS分析不同材料表面元素组成;荧光染色观察接种不同材料表面的蛋白数量及分布。DAPI染色检测HGF接种在不同材料表面2、4、6 h后早期附着细胞数。用cck8比较HGF接种不同材料表面6 h、12 h、1 d、3 d、5 d、7 d后细胞增值率。结果:XPS结果提示硅烷化组材料表面的纤维蛋白含量高于三氟组及空白组(P<0.05)。免疫荧光观察提示硅烷化组材料表面的纤维连接蛋白含量高于三氟组及空白组(P<0.05)。DAPI染色提示2 h时3组材料细胞附着数相当,4、6 h时硅烷化组材料表面上细胞数明显多于其他组材料(P<0.05)。CCK8结果提示硅烷化材料在6个观察时间段硅烷化组材料细胞增值率高于三氟组及空白组(P<0.05)。结论:硅烷化法在钛片上修饰纤维蛋白的效果优于三氟代乙烷磺酰氯的方法。

关键词: 硅烷化, 三氟代乙烷磺酰氯, 纤维连接蛋白, 牙龈成纤维细胞

Abstract: Objective: To compare the effect of modification of titanium plate with fibronectin (Fn) using three fluoroethane sulfonyl chloride and silane. Methods: Three fluoroethane sulfonyl chloride and silane were used to bond Fn on the micro grooves titanium surface. XPS was applied to analyze and compare the surface elements of different materials. Fluorescence staining was used to observe the quantity and distribution of protein on different material surfaces. DAPI staining was used to detect the early attachment of cells after HGF was seeded on different material surfaces for 2h, 4h, and 6h. The cell proliferation rates were compared by CCK-8 assay after HGF was seeded on the surface for 6h, 12h, 1d, 3d, 5d, 7d. Results: XPS results indicated that the protein content of the surface of the silane group was higher than that of the three fluorine group and the blank group (P<0.05). The content of silane group suggested that fibronectin surface immunofluorescence was higher than that of three fluorine group and blank control group (P<0.05). DAPI staining indicated that the number of cells attached to the three groups was comparable. However, after 4h and 6h, the number of cells on the surface of silane group was significantly higher than that of other two groups (P<0.05). CCK-8 results indicated that the cell proliferation rate of silane group was higher than those of three fluorine group and blank group at six time periods (P<0.05). Conclusion: The effect of silane modified method is better than three fluoroethane sulfonyl chloride to bond fibrin on the titanium plate.

Key words: Silane, Tresyl chloride, Fibronectin, Gingival fibroblast

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