口腔医学研究

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (5): 449-452.DOI: 10.13701/j.cnki.kqyxyj.2016.05.006

• 基础研究论著 • 上一篇    下一篇

复合纳米银托槽粘接剂在大鼠龋病模型上抗菌性的研究

李洁1 ,李福军2*   

  1. 1. 武汉市江夏区中医院口腔科 湖北 武汉 430200;
    2. 武汉大学口腔医学院正畸一科 湖北 武汉 430079
  • 收稿日期:2016-02-29 出版日期:2016-05-26 发布日期:2016-05-26
  • 通讯作者: 李福军,电话:(027)87686225
  • 作者简介:李洁(1981~ ), 女,学士,主治医师,主要从事口腔正畸学材料与临床研究。
  • 基金资助:
    湖北省自然科学基金(编号:2012FFB04416)

Antibacterial Properties of Nano Silver-containing Orthodontic Cements in the Rat Caries Disease Model

LI Jie1, LI Fu-jun2*.   

  1. 1. Department of Stomatology, Jiangxia Hospital of Traditional Chinese Medicine,Wuhan 430200, China;
    2. Department of Orthodontics, School of Stomatology, Wuhan University, Wuhan 430079, China.
  • Received:2016-02-29 Online:2016-05-26 Published:2016-05-26

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被引次数

1

摘要： 目的:评价复合纳米银托槽粘接剂在大鼠口内的抗菌活性。方法:利用大鼠龋病模型来模拟人的口腔环境,将托槽底板用复合纳米银托槽粘接剂粘结在上颌第一双尖牙颊侧的半边牙冠表面,再将其结扎固定在大鼠口腔下前牙舌侧的根部,分别在植入手术后的1个月和3个月时间段局麻下取25个样本牙,通过扫描电镜定期观察材料表面变形链球菌定植情况。结果:成功构建了大鼠龋病模型。通过长期的观察发现,材料样本牙放置的位置没有对其正常进食造成影响,材料几乎没有发生脱落现象。扫描电镜结果显示,Transbond XT表面变形链球菌生长活跃,层层堆积;植入1个月后的GC Fuji ORTHO LC表面变链菌零星分布或少量成簇,3个月后数量明显增多并呈链状;在NSC2,NSC3和NSC4组的两个观察期,随着纳米银含量的增加,变链菌的数量呈现减少的趋势,而且形态趋于分散单个存在。结论:纳米银的加入在某种程度上增强了粘结剂对变链菌的黏附抑制和杀灭作用。

关键词: 纳米银托槽粘接剂, 龋病模型, 抗菌性

Abstract: Objective: To evaluate the antibacterial properties of experimental nano silver-containing cements (NSC) in the rat caries disease model. Methods: Rat caries disease model was used to simulate the human oral environment. The sample teeth were bonded bracket base with adhesive onto the surface of the buccal half crowns of the first upper premolars. Then the bracket base was fixed at the lingual surface of the front teeth root in rats for a long-time preservation. After one and three months of implantation respectively, 25 samples were taken out from the mouth of the rats under local anesthesia. Then the scanning electron microscope observation was conducted. Results: The caries disease model was successfully built in rats. Through long-term observation, we found that the material samples did not affect the normal eating of rats, and there was almost no materials loss. The scanning electron microscope observation showed that the growth of streptococcus mutans was very active. One month later, Streptococcus mutans scattered or a small amount of them gathered into a cluster on GC Fuji ORTHO LC surface. While three months later, the amount of streptococcus mutans significantly increased and arranged in chains on the material surface. In the groups of NSC2, NSC3 and NSC4, the number of streptococcus mutans presented the downward trend and tended to disperse individually with the increase of nano silver content. Conclusion: The incorporation of silver nanoparticles enhanced the effect of GC Fuji ORTHO LC on the adhesion inhibition and killing of streptococcus mutans.

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