不同采样方法对口腔变形链球菌检出率的影响

doi:10.13701/j.cnki.kqyxyj.2017.10.009

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (10): 1056-1059.DOI: 10.13701/j.cnki.kqyxyj.2017.10.009

不同采样方法对口腔变形链球菌检出率的影响

尹峥嵘，孙贵军，胡慧珍，武涛，何祥一^*

兰州大学口腔医学院兰州甘肃 730000

收稿日期:2017-04-28 出版日期:2017-10-20 发布日期:2017-10-24
通讯作者: 何祥一,E-mail: hexy@lzu.edu.cn
作者简介:尹峥嵘(1996~ ),女,湖南人,学士在读,主要从事口腔微生态学研究。
基金资助:
甘肃省自然科学基金(编号:1308RJZA248)兰州大学大学生创新创业行动计划项目(编号:20151073001456)

Effect of Sampling Method on Detection of Streptococcus Mutans

YIN Zheng-rong, SUN Gui-jun, HU Hui-zhen, WU Tao, HE Xiang-yi^*

School of Stomatology, Lanzhou University, Lanzhou 730000, China.

Received:2017-04-28 Online:2017-10-20 Published:2017-10-24

摘要/Abstract

摘要： 目的:比较浓缩含漱法、非刺激性唾液法和棉拭子法对高龋和健康人群口腔唾液中变形链球菌检出率的影响。方法:对60例高龋患者和60例无龋者分别用棉拭子法、非刺激性唾液法和浓缩含漱法进行取样,采用轻唾选择培养基对变形链球菌进行培养,结合显微镜观察对3种方法收集到的变形链球菌进行鉴定,并用SPSS22.0对数据进行统计学分析。结果:3种采样方法在高龋者中检出率分别为93.33%、96.67%、91.67%,无龋者中检出率分别为56.57%、58.33%、48.33%,无统计学显著性差异。结论:3种方法口腔唾液取样一致性较好。其中,棉拭子法采集到的唾液具有良好的代表性。

关键词: 采样方法, 变形链球菌, 检出率

Abstract: Objective: To compare the detection rate of oral Streptococcus mutans in caries-active and healthy patients by concentrated rinse sampling, saliva collection, and cotton swab methods. Methods: The samples were collected from 120 patients with cotton swab, saliva collection, and concentrated rinse methods. Streptococcus mutans were cultured in Murashige & Skoog medium, and verified with microscope. The difference in various methods were analyzed by SPSS22.0.Results: The detection rates of three methods were 93.33%, 96.67%, and 91.67% in caries-active people, and 56.57%, 58.33%, and 48.33% in healthy people. Conclusion: There is no significant difference among three methods(P>0.05). Cotton swab sampling method is the representative method in clinical for its simple, reliable and convenient characteristics.

Key words: Sampling method, Streptococcus, mutans Detection rate

中图分类号:

R780.1

尹峥嵘，孙贵军，胡慧珍，武涛，何祥一. 不同采样方法对口腔变形链球菌检出率的影响[J]. 口腔医学研究, 2017, 33(10): 1056-1059.

YIN Zheng-rong, SUN Gui-jun, HU Hui-zhen, WU Tao, HE Xiang-yi. Effect of Sampling Method on Detection of Streptococcus Mutans[J]. Journal of Oral Science Research, 2017, 33(10): 1056-1059.

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不同采样方法对口腔变形链球菌检出率的影响

Effect of Sampling Method on Detection of Streptococcus Mutans

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