miR-146a-5p在慢性根尖周炎病损组织中的表达及临床意义

doi:10.13701/j.cnki.kqyxyj.2017.06.018

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (6): 650-653.DOI: 10.13701/j.cnki.kqyxyj.2017.06.018

miR-146a-5p在慢性根尖周炎病损组织中的表达及临床意义

邵丽娜, 王丝墨, 李晓琳, 仇丽鸿^*, 于博

中国医科大学口腔医学院牙体牙髓病科,辽宁省口腔医学研究所牙体牙髓病学研究室,辽宁省口腔疾病重点实验室,辽宁省口腔疾病转化医学研究中心辽宁沈阳 110002)

收稿日期:2016-11-28 出版日期:2017-06-20 发布日期:2017-06-26
通讯作者: 仇丽鸿,E-mail:drqlh@yahoo.com
作者简介:邵丽娜(1982~ ),女,辽宁沈阳人,博士在读,主治医师,主要从事牙体牙髓病学的研究工作。
基金资助:
沈阳市科学技术基金(编号:F15-199-1-56)

Expression and Clinical Significance of miR-146a-5p in Chronic Periapical Lesions.

SHAO Li-na, WANG Si-mo, LI Xiao-lin, QIU Li-hong^*, YU Bo.

Department of Endodontics, School of Stomatology, China Medical University, Liaoning Institute of Dental Research, Key Laboratory of Oral Diseases, Research Center of Translational Medicine, Liaoning 110002,China.

Received:2016-11-28 Online:2017-06-20 Published:2017-06-26

摘要/Abstract

摘要： 目的:比较慢性根尖周炎病损组织和健康牙周膜组织中miR-146a-5p的相对表达水平,探讨miR-146a-5p在慢性根尖周炎发病中的作用及临床意义。方法:收集16例诊断为慢性根尖周炎患牙的根尖周组织作为病例组,8例因正畸拔除的健康牙的牙周膜作为对照组,采用HE染色检测样本的炎症浸润程度,采用实时荧光定量PCR(real-time PCR,RT-PCR)检测miR-146a-5p的表达。所有数据采用SPSS18.0软件包进行统计学分析。结果:HE染色结果可见对照组中无明显炎症细胞浸润,病损组织中可见大量炎症细胞浸润;RT-PCR实验结果显示,病例组与对照组均有miR-146a-5p的表达,且miR-146a-5p在慢性根尖周炎病损组织中的相对表达量明显高于对照组健康牙周膜组织,差异具有统计学意义(P＜0.05)。结论:miR-146a-5p与慢性根尖周炎的发生发展密切相关。

关键词: 慢性根尖周炎, miR-146a, HE染色, 实时定量PCR

Abstract: Objective: To compare the relative expression of miR-146a-5p in chronic periapical lesions and healthy periodontal ligaments, and discuss the role and clinical significance of miR-146a-5p in the etiology of chronic apical periodontitis. Methods: Sixteen periapical tissues from chronic apical periodontitis and 8 normal periodontal ligament tissues from extracted healthy teeth for orthodontic reasons were selected. The degree of inflammatory infiltration was detected by Hematoxylin- Eosin staining. The relative expression of miR-146a-5p was detected by real-time PCR. All the data were analyzed by SPSS 18.0 software package. Results: According to the result of HE staining, there was no inflammation in control group, but large numbers of inflammatory cells were observed in periapical lesions. MiR-146a-5p was detected by real-time PCR in two groups, and the level of miR-146a-5p relative expression in periapical lesions was significantly higher than that of the healthy periodontal ligaments (P＜0.05). Conclusion: MiR-146a-5p might closely relate to the occurrence and development of chronic apical periodontitis.

Key words: Chronic apical periodontitis , miR-146a , Hematoxylin-Eosin staining , RT-PCR

中图分类号:

R781.3

邵丽娜, 王丝墨, 李晓琳, 仇丽鸿, 于博. miR-146a-5p在慢性根尖周炎病损组织中的表达及临床意义[J]. 口腔医学研究, 2017, 33(6): 650-653.

SHAO Li-na, WANG Si-mo, LI Xiao-lin, QIU Li-hong, YU Bo.. Expression and Clinical Significance of miR-146a-5p in Chronic Periapical Lesions.[J]. Journal of Oral Science Research, 2017, 33(6): 650-653.

参考文献

[1] 徐莉雅,马楠,仇丽鸿,等.慢性根尖周炎中Wnt5a与骨吸收的关系[J].口腔医学研究, 2016,32(2)∶172-176
[2] He L, Hannon GJ. MicroRNAs: small RNAs with a big role in gene regulation [J]. Nat Rev Genet, 2004, 5(7)∶522-531
[3] Williams A E, Perry M M, Moschos S A, et al. Role of miRNA-146a in the regulation of the innate immune response and cancer [J]. Biochem Soc Trans, 2008, 36(6)∶1211-1215
[4] 李晓,马瑞.根尖显微外科手术治疗慢性根尖周炎研究进展[J].中国实用口腔科杂志,2015,8(8)∶508-512
[5] Gao B, Zheng L. microRNA Expression in Rat Apical Periodontitis Bone Lesion [J]. Bone Res, 2013,1(2)∶170-185
[6] Taganov KD, Boldin MP, Chang KJ, et al. NF-kappaB dependent induction of microRNA miR-146, an inhibitor targeted to signaling proteins of innate immune responses [J]. Proc Natl Acad Sci USA, 2006, 103(33)∶12481-12486
[7] He Y, Sun X, Huang C, et al. MiR-146a regulates IL-6 production in lipopolysaccharide-induced RAW264.7 macrophage cells by inhibiting Notch1 [J]. Inflammation,2014,37(1)∶71-82
[8] Li S, Yue Y, Xu W, et al. MicroRNA-146a represses mycobacteria induced inflammatory response and facilitates bacterial replication via targeting IRAK-1 and TRAF-6 [J]. PLoS One,2013,8(12)∶e81438
[9] Taganov K D, Boldin M P, Chang K J, et al. NF-kappaB-dependent induction of microRNA miR-146, an inhibitor targeted to signaling proteins of innate immune responses [J]. Proc Natl Acad Sci USA, 2006,103(33)∶12481-12486
[10] Xie YF, Shu R, Jiang SY, et al. MicroRNA-146 inhibits proinflammatory cytokine secretion through IL-1 receptor-associated kinase 1 in human gingival fibroblasts [J]. J Inflamm ,2013,10(1)∶20-25
[11] Zheng T, Chou J, Zhang F, et al. CXCR4 3'UTR functions as a ceRNA in promoting metastasis, proliferation and survival of MCF-7 cells by regulating miR-146a activity [J]. Eur J Cell Biol, 2015,94(10)∶458-469
[12] Xu B, Huang Y, Niu X, et al. Hsa-miR-146a-5p modulates androgen-independent prostate cancer cells apoptosis by targeting ROCK1 [J]. Prostate,2015,75(16)∶1896-1903
[13] Zhou W, Thiery J P. Loss of Git2 induces epithelial-mesenchymal transition by miR146a-Cnot6L-controlled expression of Zeb1 [J]. J Cell Sci,2013,126(12)∶2740-2746
[14] Hung PS, Chen FC, Kuang SH, et al. miR-146a induces differentiation of periodontal ligament cells [J]. J Dent Res,2010, 89 (3)∶252-257

miR-146a-5p在慢性根尖周炎病损组织中的表达及临床意义

Expression and Clinical Significance of miR-146a-5p in Chronic Periapical Lesions.

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