口腔医学研究

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (11): 1204-1208.DOI: 10.13701/j.cnki.kqyxyj.2017.11.018

• 口腔肿瘤研究 • 上一篇    下一篇

miR-146a在口腔鳞癌组织及细胞中的表达及功能研究

王丽萍, 郭雪琪, 闫勇勇, 査骏, 陈伟鸿, 魏永祥, 朱昕欣, 管红兵, 葛林虎*   

  1. 广州医科大学附属口腔医院,广州口腔疾病研究所,口腔医学重点实验室 广东 广州 510140
  • 收稿日期:2017-06-11 出版日期:2017-11-20 发布日期:2017-11-29
  • 通讯作者: 葛林虎
  • 作者简介:王丽萍(1969~ ),女,浙江人,学士,主任医师,主要从事口腔种植的临床治疗工作及相关研究。
  • 基金资助:
    广东省科学技术厅基金项目(编号:2014807-49)
    广州市荔湾区科技信息化局基金项目(编号:20141216070)

Effect and Functioanl Role of miR-146a in Oral Squamous Cell Carcinoma Tissues and Cell Lines

WANG Li-ping, GUO Xue-qi, YAN Yong-yong, ZHA Jun, CHEN Wei-hong, WEI Yong-xiang, ZHU Xin-xin, GUAN Hong-bing, GE Lin-hu*   

  1. Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou 510140, China
  • Received:2017-06-11 Online:2017-11-20 Published:2017-11-29

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摘要： 目的:探究miR-146a在口腔鳞状细胞癌(OSCC)组织及细胞中的表达及其对口腔鳞癌细胞生物学行为能力的影响。方法:采用荧光定量聚合酶链反应(RT-PCR)法检测OSCC组织及细胞中miR-146a的表达,观察miR-146a对OSCC细胞增殖、侵袭、迁移等能力的影响。结果:与癌旁组织相比OSCC组织中miR-146a呈明显高表达(P<0.05);与正常口腔上皮细胞HOK相比,OSCC细胞系Scc9、Scc25、Cal27中miR-146a表达水平明显偏高(P<0.05)。上调miR-146a表达后,Scc9及Cal27的细胞增殖能力明显增加(P<0.05),Scc9、Scc25、Cal27的侵袭和迁移能力明显增加(P<0.05);抑制miR-146a表达后,Scc9、Scc25、Cal27的侵袭和迁移能力明显降低(P<0.05)。结论:miR-146a在OSCC和口腔癌细胞中呈明显高表达,对于促进口腔癌的增殖、侵袭和迁移具有重要意义。

关键词: miR-146a, 口腔鳞状细胞癌, miRNA

Abstract: Objective: To explore the expression and its functional role of miR-146a in oral squamous cell carcinoma (OSCC) tissues. Methods: RT-PCR was performed to detect the expression level of miR-146a in OSCC tissues and OSCC cell lines. CCK8 assay, transwell assay and wound healing assay were performed to observer cell proliferation, invasion and migration abilities. Results: The expression of miR-146a was significantly higher in OSCC tissues compared to the adjacent tissues (P<0.05), and the expression of miR-146a in OSCC cell lines such as Scc9, Scc25, and Cal27 were substantially higher than the normal oral epithelial cells (HOK) (P<0.05). The proliferation, invasion and migration abilities were substantially enhanced in Scc9 and Cal27 cells transfected with miR-146a mimics (P<0.05). The invasion and migration abilities were substantially decreased in Scc9, Scc25, and Cal27 cells transfected with miR-146a inhibitor (P<0.05), but the proliferation abilities showed no statistically diffierents in Scc9, Scc25, and Cal27 cells transfected with miR-146a inhibitor. Conclusion: miR-146a shows a significant expression in OSCC tissues and cell lines, is correlated with proliferation, invasion and migration of OSCC.

Key words: miR-146a, OSCC, miRNA

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