牙周健康状况和民族因素对口腔微生物多样性的协同作用

doi:10.13701/j.cnki.kqyxyj.2017.07.014

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (7): 745-749.DOI: 10.13701/j.cnki.kqyxyj.2017.07.014

牙周健康状况和民族因素对口腔微生物多样性的协同作用

马瑞¹, 施文¹, 易根云¹, 张小凤¹, 黄慧敏¹, 周建业², 余占海^1*, 李志强^2*

1. 兰州大学口腔医学院甘肃兰州 730000;
2. 西北民族大学口腔医学院甘肃兰州 730000

收稿日期:2016-12-18 出版日期:2017-07-20 发布日期:2017-07-27
通讯作者: 余占海,E-mail:yuzhanhai@lzu.edu.cn李志强,E-mail:xbmulzq@163.com
作者简介:马瑞(1988~ ),女,河南人,硕士,主要从事口腔微生物群落结构多样性及β-TCP煅烧骨的研究。
基金资助:
国家自然科学基金(编号:31560159/C0309;31360124/C0309)

Co-effects of Ethnicity and Periodontal Health Status on Oral Microbiome Diversity.

MA Rui¹, SHI Wen¹, YI Gen-yun¹, ZHANG Xiao-feng¹, HUANG Hui-min¹, ZHOU Jian-ye², YU Zhan-hai^1*, LI Zhi-qiang^2*.

1. School of Stomatology, Lanzhou University, Lanzhou 730000, China;
2. School of Stomatology, Northwest University for Nationalities, Lanzhou 730000,China.

Received:2016-12-18 Online:2017-07-20 Published:2017-07-27

摘要/Abstract

摘要： 目的:通过高通量测序探寻牙周健康状况和民族对口腔微生物多样性产生的影响。方法:采集12名牙周炎患者(5名汉族和7名裕固族)和10名健康者(5名汉族和5名裕固族)的唾液,提取细菌总DNA,用Illumina MiSeq高通量测序平台对16S rRNA基因序列进行测序分析。结果:在UniFrac距离总变化中,牙周因素在未加权分析中有差异(P=0.02),民族因素在加权分析中有差异(P=0.03),两种因素的相互作用在加权和未加权分析中均有差异(P<0.05)。结论:牙周健康状况及民族因素对口腔微生物多样性均有影响作用,并可能存在协同作用,但牙周健康状况是引起细菌组成变化的主要因素。

关键词: 民族, 牙周健康状况, α-多样性, β-多样性, 微生物组成

Abstract: Objective: To explore the effects influenced by the status of periodontal health and different ethnicity on oral microbial diversity by high throughput sequencing. Methods: Saliva was collected from 12 periodontitis patients (5 Han and 7 Yugur) and 10 healthy sub-jects (5 Han and 5 Yugur). The total DNA of bacterias was extracted, then the sequence of 16S rRNA gene was sequenced by Illumina MiSeq high throughput sequencing platform. Results: In the UniFrac distance total variation observation, the periodontal factors only explained the variation in the unweighted analysis, and the ethnic factors just explained the variation in the weighted analysis. The interaction of the two influencing factors explained the variation in both unweighted and weighted analysis. Conclusion: Periodontal health status and ethnic factors have effects on oral microbial diversity, and there may be synergistic effects, but periodontal health status is the main factor in changing the bacterial composition.

Key words: Ethnicity, Periodontal health status, α-diversity , β-diversity , Microbial composition

中图分类号:

R781.4

马瑞, 施文, 易根云, 张小凤, 黄慧敏, 周建业, 余占海, 李志强. 牙周健康状况和民族因素对口腔微生物多样性的协同作用[J]. 口腔医学研究, 2017, 33(7): 745-749.

MA Rui, SHI Wen, YI Gen-yun, ZHANG Xiao-feng, HUANG Hui-min, ZHOU Jian-ye, YU Zhan-hai, LI Zhi-qiang.. Co-effects of Ethnicity and Periodontal Health Status on Oral Microbiome Diversity.[J]. Journal of Oral Science Research, 2017, 33(7): 745-749.

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牙周健康状况和民族因素对口腔微生物多样性的协同作用

Co-effects of Ethnicity and Periodontal Health Status on Oral Microbiome Diversity.

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