口腔医学研究

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (8): 824-827.DOI: 10.13701/j.cnki.kqyxyj.2017.08.006

• 基础研究论著 • 上一篇    下一篇

多囊蛋白-1在生长期小鼠颅底蝶枕软骨联合的表达

高国杰1,2, 章筱悦1, 沈刚1*   

  1. 1. 上海交通大学医学院第九人民医院正畸科 上海 200011;
    2. 昆明医科大学口腔医学院正畸科 云南 昆明 650031
  • 收稿日期:2016-11-18 出版日期:2017-08-20 发布日期:2017-08-28
  • 通讯作者: 沈刚,E-mail:gangshen@orthosh.com
  • 作者简介:高国杰(1976~ ),男,云南昆明人,博士,主治医师,主要从事口腔正畸临床与基础研究。
  • 基金资助:
    国家自然科学基金(编号:81271182,81470765)

Expression of Polycystin-1 in Cranial Base Spheno-occipital Synchondrosis of Growing Mouse.

GAO Guo-jie1,2,ZHANG Xiao-yue1,SHEN Gang1*.   

  1. 1. Department of Orthodontics, The Ninth People'
    s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China;
    2. Department of Orthodontics, School of Stomatology, Kunming Medical University, Kunming 650031, China.
  • Received:2016-11-18 Online:2017-08-20 Published:2017-08-28

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摘要： 目的:了解多囊蛋白-1(PC1)在不同生长阶段小鼠蝶枕软骨联合表达的时空特征。方法:采集1、4、8周龄小鼠的蝶枕软骨联合标本,制备组织切片后进行PC1免疫组织化学染色,观察PC1的表达位置并测算单位面积组织内PC1阳性面积的百分比。结果:PC1在1周龄小鼠蝶枕软骨联合表达于储备层、前肥大层和早期肥大层;4周龄时主要表达于储备层、增殖层和前肥大层;8周龄时主要表达于前肥大细胞和早期肥大细胞。1、4、8周龄组PC1阳性面积百分比的均值分别为4.51%、5.7%、4.59%;4周龄组与1周龄及8周龄组间的差异都具有显著性(P<0.05)。结论:生长期小鼠蝶枕软骨联合表达PC1,但不同生长阶段的表达区域和水平存在差异。

关键词: 多囊蛋白-1, 小鼠, 颅底, 蝶枕软骨联合

Abstract: Objective: To investigate the tempro-spatial characters of polycystin-1 (PC1) expression in mouse spheno-occipital synchondrosis (SOS) at different growth stages. Methods: Tissue sections of SOS of 1, 4,8 weeks postnatal mouse were prepared, then the expression of PC1 was detected with immunohistochemical staining and the percentage of positive area to synchondrosis area was calculated. Results: At the age of 1 week, the expression of PC1 in SOS was observed in reserve zone, pre-hypertrophy zone, and early hypertrophy zone; at 4 weeks, PC1 expression in reserve zone, proliferation zone, and pre-hypertrophy zone was obviously enhanced; at 8 weeks, the PC1 expression was concentrated in pre-hypertrophy zone and hypertrophy zone. The mean percentages of positive area were 4.51%, 5.7% and 4.59% at 1week, 4 weeks and 8 weeks, respectively. The difference between 4-week-old group and any other group was significant (P<0.05). Conclusion: PC1 is expressed in spheno-occipital synchondrosis of growing mouse, but its site and level vary at different growth stages.

Key words: Polycystin-1 , Mouse Cranial base, Spheno-occipital synchondrosis

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