PTEN对H2O2诱导的牙周膜细胞增殖凋亡的影响

doi:10.13701/j.cnki.kqyxyj.2017.08.022

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (8): 889-892.DOI: 10.13701/j.cnki.kqyxyj.2017.08.022

PTEN对H₂O₂诱导的牙周膜细胞增殖凋亡的影响

赵媛¹, 王姝², 韶波³, 高颖^1*

1. 郑州大学附属郑州中心医院口腔颌面外科河南郑州 450007;
2. 内蒙古医科大学附属医院口腔颌面外科内蒙古呼和浩特 010000;
3. 内蒙古自治区人民医院口腔科内蒙古呼和浩特 010000

收稿日期:2017-03-18 出版日期:2017-08-20 发布日期:2017-08-28
通讯作者: 高颖,电话:13838225731
作者简介:赵媛(1982~ ),女,黑龙江人,硕士,主治医师,主要从事口腔颌面外科的临床治疗工作。
基金资助:
河南省医学科技攻关计划项目(编号:201303100);内蒙古医科大学青年创新基金项目 (编号:YKD2015QNCX042)

Effects of PTEN on Proliferation and Apoptosis of Periodontal Ligament Cells Induced by Hydrogen Peroxide.

ZHAO Yuan¹, WANG Shu², SHAO Bo³, GAO Ying^1*

1. Department of Oral and Maxillofacial Surgery, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 4570007, China;
2. Department of Oral and Maxillofacial Surgery, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010000, China;
3. Department of Stomatology, The Inner Mongolia Autonomous Region People'
s Hospital, Hohhot 010000, China.

Received:2017-03-18 Online:2017-08-20 Published:2017-08-28

摘要/Abstract

摘要： 目的:探讨第10号染色体同源缺失性磷酸酶-张力蛋白(PTEN)对过氧化氢(H₂O₂)诱导的牙周膜细胞增殖凋亡的影响。方法:分离培养人牙周膜细胞,转染PTEN小干扰RNA、小干扰RNA阴性对照,H₂O₂处理,Western blot检测PTEN表达水平。结果:人牙周膜细胞转染PTEN小干扰RNA后的细胞中PTEN表达受到抑制。结论:干扰PTEN表达能够拮抗 H₂O₂诱导的人牙周膜细胞凋亡及人牙周膜细胞增殖抑制作用。

关键词: 牙周膜细胞, 增殖, 凋亡, 第10号染色体同源缺失性磷酸酶-张力蛋白, 过氧化氢

Abstract: Objective: To investigate the effects of PTEN on proliferation and apoptosis of periodontal ligament cells induced by hydrogen peroxide. Methods: Human periodontal ligament cells were isolated and cultured in vitro, and then transfected with PTEN small interfering RNA or small interfering RNA negative control. After H₂O₂ treatment, PTEN expression was detected in cells by Western blot. Results: PTEN expression was inhibited in human periodontal ligament cells transfected with PTEN small interfering RNA. Conclusion: Interference of PTEN expression could inhibit the apoptosis and the proliferation of human periodontal ligament cells induced by H₂O₂.

Key words: Periodontal ligament cells, Proliferation, Apoptosis , Phosphatase and tensin homolog deleted on chromosome ten, Hydrogen peroxide

中图分类号:

R780.1

赵媛, 王姝, 韶波, 高颖. PTEN对H₂O₂诱导的牙周膜细胞增殖凋亡的影响[J]. 口腔医学研究, 2017, 33(8): 889-892.

ZHAO Yuan, WANG Shu, SHAO Bo, GAO Ying. Effects of PTEN on Proliferation and Apoptosis of Periodontal Ligament Cells Induced by Hydrogen Peroxide.[J]. Journal of Oral Science Research, 2017, 33(8): 889-892.

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PTEN对H₂O₂诱导的牙周膜细胞增殖凋亡的影响

Effects of PTEN on Proliferation and Apoptosis of Periodontal Ligament Cells Induced by Hydrogen Peroxide.

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