载PTH-Fc/PLCL/SF静电纺丝屏障膜的构建与性能研究

doi:10.13701/j.cnki.kqyxyj.2018.08.027

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (8): 913-918.DOI: 10.13701/j.cnki.kqyxyj.2018.08.027

• 口腔生物材料研究 • 上一篇

载PTH-Fc/PLCL/SF静电纺丝屏障膜的构建与性能研究

朱艺丹, 何苗苗, 刘加涛, 刘艳云, 刘玉慧, 徐宁, 唐一月, 余占海^*

兰州大学口腔医学院甘肃兰州 730000

收稿日期:2018-01-09 出版日期:2018-08-28 发布日期:2018-08-23
通讯作者: 余占海,E-mail:yuzhanhai@lzu.edu.cn
作者简介:朱艺丹(1992~ ),女,新疆人,硕士,主要从事口腔种植学研究。

Fabrication and Characterization of Fibrous Barrier Mat of Poly(L-lactide-co-caprolactone)/silk Fibroin Loaded with PTH-Fc

ZHU Yi-dan, HE Miao-miao, LIU Jia-tao, LIU Yan-yun, LIU Yu-hui, XU Ning, TANG Yi-yue, YU Zhan-hai^*

School and Hospital of Stomatology, Lanzhou University, Lanzhou 730000, China

Received:2018-01-09 Online:2018-08-28 Published:2018-08-23

摘要/Abstract

摘要： 目的:利用静电纺丝技术制备携载PTH-Fc的PLCL/SF三维纤维纳米屏障膜,并研究其生物相容性、生物降解能力、机械强度和调节细胞活动的能力。方法:制备PTH-Fc/PLCL/SF屏障膜,并与PLCL/SF屏障膜对照,扫描电镜研究其降解前后微观形貌,拉伸实验研究其机械强度,MTT测试与细胞粘附性实验研究屏障膜的生物相容性,茜素红染色研究携载PTH-Fc缓释是否可以影响细胞成骨分化。结果:PTH-Fc/PLCL/SF屏障膜纳米纤维丝均一光滑。PTH-Fc/PLCL/SF与PLCL/SF屏障膜断裂强度为(5.56±0.18) MPa、(5.42±0.03) MPa,两者无统计学意义。PTH-Fc/PLCL/SF生物降解能力优异,PTH-Fc/PLCL/SF与PLCL/SF屏障膜均可以促进细胞增殖(P＜0.05),两者之间无统计学意义,但PTH-Fc/PLCL/SF能促进细胞分化。结论:PTH-Fc/PLCL/SF屏障膜有良好的机械强度、适宜的降解能力,能促进BMMSCs细胞增殖与分化。

关键词: 静电纺丝, PTH-Fc, 引导骨再生, 屏障膜

Abstract: Objective: To fabricate fibrous barrier mat of poly(L-lactide-co-caprolactone)/silk fibroin loaded with PTH-Fc via electrospinning technique, and to study its biocompatibility, biodegradability, mechanical strength, and ability to regulate appropriate cellular activities. Methods: Electrospun fibrous poly(L-lactide-co-caprolactone)/silk fibroin loaded with PTH-Fc (PLCL/SF/PTH-Fc) or without (PLCL/SF) were prepared and compared. Apparent morphology before and after degradation were observed by scanning electron microscope (SEM). Tensile property was tested by custom mechanical testing device. The biocompatibility of PLCL/SF/PTH-Fc was evaluated by MTT and the cell adhesion assay. The osteogenic potential of the cells cultured with PLCL/SF/PTH-Fc was investigated by alizarin red stain. Results: The nanofibers of PLCL/SF/PTH-FC were uniform and smooth. The breaking strengths of PLCL/SF/PTH-Fc and PLCL/SF were (5.56±0.18) MPa and (5.42±0.03) MPa, respectively (P>0.05). PLCL/SF/PTH-Fc mats could degrade quickly, promote the cell proliferation, and induce the cell differentiation. Conclution: Electrospun fibrous PLCL/SF/PTH-Fc has good mechanical property and biodegradability. It can promote cell proliferation and cell differentiation.

Key words: Electrospinning, PTH-Fc, Guided bone regeneration, Fibrous barrier

朱艺丹, 何苗苗, 刘加涛, 刘艳云, 刘玉慧, 徐宁, 唐一月, 余占海. 载PTH-Fc/PLCL/SF静电纺丝屏障膜的构建与性能研究[J]. 口腔医学研究, 2018, 34(8): 913-918.

ZHU Yi-dan, HE Miao-miao, LIU Jia-tao, LIU Yan-yun, LIU Yu-hui, XU Ning, TANG Yi-yue, YU Zhan-hai. Fabrication and Characterization of Fibrous Barrier Mat of Poly(L-lactide-co-caprolactone)/silk Fibroin Loaded with PTH-Fc[J]. Journal of Oral Science Research, 2018, 34(8): 913-918.

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载PTH-Fc/PLCL/SF静电纺丝屏障膜的构建与性能研究

Fabrication and Characterization of Fibrous Barrier Mat of Poly(L-lactide-co-caprolactone)/silk Fibroin Loaded with PTH-Fc

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