PTH-FC对大鼠骨髓间充质干细胞增殖及骨向分化的影响

doi:10.13701/j.cnki.kqyxyj.2018.06.010

口腔医学研究 ›› 2018, Vol. 34 ›› Issue (6): 614-618.DOI: 10.13701/j.cnki.kqyxyj.2018.06.010

PTH-FC对大鼠骨髓间充质干细胞增殖及骨向分化的影响

刘艳云, 徐宁, 刘玉慧, 刘加涛, 朱艺丹, 何苗苗, 余占海^*

兰州大学口腔医学院(医院)种植科甘肃兰州 730000

收稿日期:2017-12-29 出版日期:2018-06-20 发布日期:2018-06-21
通讯作者: 余占海, E-mail:yuzhanhai@lzu.edu.cn
作者简介:刘艳云(1990～),女,陕西渭南人,硕士在读,主要从事口腔种植和基础研究。
基金资助:
兰州市人才创新创业项目(编号:2014-RC-79)

Effects of PTH-FC on Proliferation and Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells

LIU Yan-yun, XU Ning, LIU Yu-hui, LIU Jia-tao, ZHU Yi-dan, HE Miao-miao, YU Zhan-hai^*

Department of Implantology, School and Hospital of Stomatology, Lanzhou University, Lanzhou 730000, China

Received:2017-12-29 Online:2018-06-20 Published:2018-06-21

摘要/Abstract

摘要： 目的:评价甲状旁腺激素融合蛋白PTH-FC(parathyroid hormone -Fc)对大鼠骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)骨向分化的作用,筛选出PTH-FC诱导BMSCs最佳的作用时间。方法:采用全骨髓贴壁法分离培养大鼠BMSCs并进行鉴定,以1.0×10^-9 mol/L PTH-FC成骨诱导液诱导BMSCs作用6 h(间歇性给药组)和48 h(持续性给药组)通过噻唑蓝(methylthiazolyldiphenyl-tetrazolium bromide, MTT)、碱性磷酸酶(alkaline phosphatase,ALP)、茜素红染色观察PTH-FC对BMSCs增殖和骨向分化的作用。结果:含1.0×10^-9 mol/L PTH-FC的成骨培养液作用6 h间歇性给药组与其他组比较,ALP和茜素红染色结果显著高于其他组;MTT结果显示各实验组间比较差异无统计学意义。结论:1.0×10^-9 mol/L PTH-FC作用BMSCs 6h的间歇性给药组具有最佳的骨向诱导能力,这提示PTH-FC作为骨诱导生长因子联合BMSCs对骨组织缺损的修复是可行的。

关键词: 甲状旁腺激素融合蛋白PTH-FC, 骨髓间充质干细胞, 骨向分化

Abstract: Objective: To evaluate the effects of PTH-FC on osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs), and to screen out the optimal time of BMSCs induced by PTH-FC. Methods: BMSCs from rats were isolated and cultured in vitro using the whole bone marrow adherence method and identified. BMSCs was induced with PTH-FC (10^-9mol/L) osteogenic inducer for 6h (intermittent administration) and 48h (continuous administration). The best proliferation promoting and osteogenic differentiation promoting effect of PTH-FC on BMSCs was evaluated by means of MTT, ALP, and alizarin red staining. Results: ALP and alizarin red staining in the osteogenic culture medium of PTH-FC (10^-9mol/L) which acted on BMSCs for 6h were significantly higher than those of other groups (P<0.05), however, the cell proliferation result was not significant different between the experimental groups. Conclusion: The combination of PTH-FC, which is used as a bone induced growth factor, with BMSCs to repair bone tissue defects is feasible.

Key words: Parathyroid hormone-FC, Bone marrow mesenchymal stem cells, Osteogenic differentiation

刘艳云, 徐宁, 刘玉慧, 刘加涛, 朱艺丹, 何苗苗, 余占海. PTH-FC对大鼠骨髓间充质干细胞增殖及骨向分化的影响[J]. 口腔医学研究, 2018, 34(6): 614-618.

LIU Yan-yun, XU Ning, LIU Yu-hui, LIU Jia-tao, ZHU Yi-dan, HE Miao-miao, YU Zhan-hai. Effects of PTH-FC on Proliferation and Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells[J]. Journal of Oral Science Research, 2018, 34(6): 614-618.

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PTH-FC对大鼠骨髓间充质干细胞增殖及骨向分化的影响

Effects of PTH-FC on Proliferation and Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells

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