口腔医学研究

口腔医学研究 ›› 2020, Vol. 36 ›› Issue (1): 79-84.DOI: 10.13701/j.cnki.kqyxyj.2020.01.019

• 口腔生物学研究 • 上一篇    下一篇

白藜芦醇通过沉默信息调节因子/叉头转录因子通路对高糖诱导成骨细胞增殖、分化的影响

唐晓悦1, 朱浩2, 曹灿3*   

  1. 1. 中国人民解放军北部战区总医院和平分院口腔科 辽宁 沈阳 110000;
    2. 中国人民解放军北部战区总医院口腔颌面外科 辽宁 沈阳 110000;
    3. 中国人民解放军北部战区总医院口腔科 辽宁 沈阳 110000
  • 收稿日期:2019-04-24 出版日期:2020-01-28 发布日期:2020-01-16
  • 通讯作者: 曹灿,E-mail:517592181@qq.com
  • 作者简介:唐晓悦(1988~ ),女,辽宁沈阳人,硕士,主治医师,研究方向:口腔成骨合和口腔正畸学研究。
  • 基金资助:
    辽宁省科学技术计划项目(编号:201601404)

Effects of Resveratrol on Proliferation and Differentiation of Osteoblasts Induced by High Glucose through SIRT1/FOXO1 Pathway

TANG Xiaoyue1, ZHU Hao2, CAO Can3*   

  1. 1. Department of Stomatology, Peace Branch, General Hospital of the Northern War Zone of the Chinese People's Liberation Army, Shenyang 110000, China;
    2. Department of Oral and Maxillofacial Surgery, General Hospital of the Northern War Zone of the Chinese People's Liberation Army, Shenyang 110000, China;
    3. Department of Stomatology, General Hospital of the Northern War Zone of the People's Liberation Army, Shenyang 110000, China
  • Received:2019-04-24 Online:2020-01-28 Published:2020-01-16

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摘要： 目的:探究白藜芦醇(resveratrol,Res)对高糖诱导成骨细胞增殖、分化的影响及其对沉默信息调节因子(silence information regulator 1,sirt1)/叉头转录因子(forkhead transcription factor 1,FOXO1)通路的影响。方法:分离培养大鼠下颌骨细胞,分为control组、高糖组、高糖+低剂量Res组、高糖+中剂量Res组、高糖+高剂量Res组,细胞计数试剂盒(cell counting kit-8,CCK-8)法检测细胞增殖情况,碱性磷酸酶(alkaline phosphatase,ALP)染色测定ALP活性,Alizarin red S染色检测钙化结节形成,Western blot法检测Ⅰ型胶原(Collagen typeⅠ,CoI)、Runt相关转录因子2(Runt-related transcription factor 2,Runx2)、骨桥蛋白(osteopontin,OPN)、骨钙素(osteocalcin,OCN)蛋白及sirt1/FOXO1通路蛋白表达情况。结果:与control组比较,高糖组成骨细胞增殖抑制率增加,高糖组ALP活性、钙结节沉积、CoI、RUNX2、OPN、OCN蛋白表达水平显著降低(P＜0.05);与高糖组比较,白藜芦醇添加组成骨细胞的A值、ALP活性、钙结节沉积、CoI、RUNX2、OPN、OCN蛋白表达水平显著增加(P＜0.05),且随着浓度的增加而增加;与control组比较,高糖组成骨细胞sirt1、p27蛋白表达水平显著降低,FOXO1、Bim蛋白表达水平显著升高(P＜0.05);与高糖组比较,加入白藜芦醇后sirt1、p27蛋白表达水平明显升高,FOXO1、Bim蛋白表达水平显著降低(P＜0.05)。结论:白藜芦醇可能通过调节sirt1/FOXO1通路,改善高糖对成骨细胞增殖、分化的抑制作用。

关键词: 白藜芦醇, 沉默信息调节因子/叉头转录因子通路, 成骨细胞, 增殖, 分化

Abstract: Objective: To investigate the effects of resveratrol (Res) on the proliferation and differentiation of osteoblasts induced by high glucose and on the silence information regulator (SIRT1)/fork transcription factor (FOXO1) pathway. Methods: Rat mandibular cells were isolated and cultured, and the experiment was divided into control group, high-glucose group, high-glucose + low-dose Res group, high-glucose + medium-dose Res group, and high-glucose + high-dose Res group. CKK-8 assay was used to detect cell proliferation, ALP activity was determined by alkaline phosphatase (ALP) staining, Alizarin red S staining was used to detect the formation of calcified nodules and, Western blotting was used to detect the expressions of type I collagen (COLI) and RUNX2, osteopontin (OPN), osteocalcin (OCN), and SIRT1/FOXO1 pathway protein. Results: Compared with control group, inhibition in high-glucose group increased significantly, ALP activity, calcium nodule deposition, COLI, RUNX2, OPN, and OCN protein levels in high-glucose group decreased significantly (P<0.05). Compared with the high-glucose group, the OD value, ALP activity, calcium nodule deposition, COLI, RUNX2, OPN, and OCN protein levels of osteoblasts in resve- ratrol supplemented groups increased significantly (P<0.05), and increased with the increase of concentration. Compared with control group, the levels of SIRT1 and P27 protein of osteoblasts in high-glucose group decreased significantly, while the levels of FOXO1 and Bim increased significantly (P<0.05). Compared with the high-glucose group, the levels of SIRT1 and P27 protein increased significantly and FOXO1 and Bim protein decreased significantly after adding resveratrol (P<0.05). Conclusion: Resveratrol may improve the inhibitory effect of high glucose on osteoblasts proliferation and differentiation by adjusting SIRT1/FOXO1 pathway.

Key words: resveratrol, SIRT1/FOXO1 pathway, osteoblasts, proliferation, differentiation