牙龈卟啉单胞菌脂多糖对人脐静脉内皮细胞表达小凹蛋白-1的影响

doi:10.13701/j.cnki.kqyxyj.2020.01.018

口腔医学研究 ›› 2020, Vol. 36 ›› Issue (1): 75-78.DOI: 10.13701/j.cnki.kqyxyj.2020.01.018

牙龈卟啉单胞菌脂多糖对人脐静脉内皮细胞表达小凹蛋白-1的影响

左源, 李维善^*

佳木斯大学附属口腔医院牙周黏膜病科黑龙江佳木斯 154007

收稿日期:2019-04-10 出版日期:2020-01-28 发布日期:2020-01-16
通讯作者: 李维善,E-mail:weishanli666@126.com
作者简介:左源(1994~ ),女,黑龙江佳木斯人,硕士在读,主要从事口腔牙周黏膜病学的临床及基础研究工作。
基金资助:
黑龙江省教育厅科研基金(编号:12531707)

Effect of Porphyromonas Gingivalis Lipopolysaccharide on Expression of Cav-1 in HUVECs

ZUO Yuan,LI Weishan^*

Department of Periodontal Membrane,Stomatological Hospital Affiliated to Jiamusi University,Jiamusi 154007, China

Received:2019-04-10 Online:2020-01-28 Published:2020-01-16

摘要/Abstract

摘要： 目的:初步研究牙龈卟啉单胞菌脂多糖(P.gingivalis lipopolysaccharide,P.g-LPS)对人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs)小凹蛋白-1(caveolin-1,Cav-1)表达的影响,并探讨P.g -LPS与动脉粥样硬化(atherolerosis,AS)之间的关系。方法:体外培养HUVECs,选择不同浓度的P.g -LPS(0.5、1.0、5.0、10.0 mg/L)诱导刺激6、18、24、36 h,采用5-乙炔基-2’脱氧尿嘧啶核苷(5-ethynyl-2’-deoxyuridine,EdU)法检测P.g -LPS对HUVECs增殖的影响,酶联免疫吸附实验(enzyme linked immunosorbent assay,ELISA)检测HUVECs表达Cav-1的量,采用逆转录-聚合酶链反应(reverse transcription-polymerase chain reaction ,RT-PCR)检测Cav-1 mRNA的表达。结果:P.g -LPS与HUVECs共培养后,增殖活力较对照组下降,并随着P.g-LPS浓度增加,HUVECs增殖活力明显下降(P＜0.05)。与对照组比较,P.g -LPS诱导HUVECs Cav-1蛋白表达在18 h可升高,24、36 h持续高表达,36 h表达水平最高(P＜0.05);P.g-LPS诱导HUVECs Cav-1 mRNA表达在18 h可升高,24、36 h随着浓度的增加,Cav-1 mRNA表达量增加(P＜0.001)。结论:P.g-LPS能够明显抑制HUVECs的增殖活性,上调细胞Cav-1的蛋白以及分子水平表达。提示P.g-LPS感染可能引起内皮细胞功能障碍,从而加速AS的发生与发展。

关键词: 牙龈卟啉单胞菌, 脂多糖, 人脐静脉内皮细胞, 小凹蛋白-1, 动脉粥样硬化

Abstract: Objective: To study the effect of P. gingivalis lipopolysaccharide (P. g-LPS) on the expression of caveolin-1 (Cav-1) in human umbilical vein endothelial cells (HUVECs), and to explore the relationship between P. g-LPS and atherosclerosis (AS). Methods: HUVECs were cultured in vitro, and different concentrations of P.g-LPS (0.5, 1.0, 5.0, 10.0 mg/L) were selected to induce for 6, 18, 24 and 36 hours. The effects of P.g-LPS on the proliferation of HUVECs were detected by EdU method, the expression of Cav-1 in HUVECs was detected by ELISA, and the expression of Cav-1 was detected by RT-PCR. Results: After co-culture of P.g-LPS and HUVECs, the proliferation activity of HUVECs decreased compared with the control group. With the increase of P.g-LPS concentration, the proliferation activity of HUVECs decreased significantly (P<0.05). Compared with the control group, the expression of Cav-1 protein in HUVECs induced by P.g-LPS increased for 18 hours, sustained high expression for 24 and 36 hours, with the highest expression level at 36 hours (P<0.05); the expression of Cav-1 gene in HUVECs induced by P.g-LPS increased for 18 hours, and the expression of Cav-1 gene increased with the increase of concentration at 24 and 36 hours (P<0.001). Conclusion: P.g-LPS can significantly inhibit the proliferation activity of HUVECs and up-regulate the protein and molecular level of Cav-1 cells. It is suggested that P.g-LPS infection may cause endothelial cell disfunction, thereby accelerating the occurrence and development of AS

Key words: porphyromonas gingivalis, lipopolysaccharide, human umbilical vein endothelial cells, caveolin-1 atherolerosis

左源, 李维善. 牙龈卟啉单胞菌脂多糖对人脐静脉内皮细胞表达小凹蛋白-1的影响[J]. 口腔医学研究, 2020, 36(1): 75-78.

ZUO Yuan,LI Weishan. Effect of Porphyromonas Gingivalis Lipopolysaccharide on Expression of Cav-1 in HUVECs[J]. Journal of Oral Science Research, 2020, 36(1): 75-78.

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牙龈卟啉单胞菌脂多糖对人脐静脉内皮细胞表达小凹蛋白-1的影响

Effect of Porphyromonas Gingivalis Lipopolysaccharide on Expression of Cav-1 in HUVECs

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