口腔医学研究

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (6): 593-596.DOI: 10.13701/j.cnki.kqyxyj.2017.06.004

• 基础研究论著 • 上一篇    下一篇

周期性流体静压力对BMSCs细胞增殖活性及超微结构的影响

潘景光1, 赵萤2, 刘岩正1, 张旻2*   

  1. 1. 解放军第518医院口腔中心 陕西 西安 710043;
    2. 军事口腔医学国家重点实验室,国家口腔疾病临床医学研究中心,陕西省口腔疾病国际联合研究中心,第四军医大学急诊与综合临床科 陕西 西安 710032)
  • 收稿日期:2016-12-20 出版日期:2017-06-20 发布日期:2017-06-26
  • 通讯作者: 张旻,E-mail: zhangmin@fmmu.edu.cn
  • 作者简介:潘景光(1973~ ),男,黑龙江北安人,主任医师,博士,主要从事口腔修复学研究。
  • 基金资助:
    国家自然科学基金(编号:81371188、31570951)

Effects of cyclic hydrostatic pressure on proliferation and ultrastructure of rat’s BMSCs.

PAN Jing-guang1, ZHAO Yin2, LIU Yan-zheng1, ZHANG Min2*.   

  1. 1. Department of StomatoIogy,The 518th Military Hospital,Xi'
    an 710043,China;
    2. State Key Laboratory of Military Stomatology, National Center for oral diseases and clinical medicine, Department of General Dentistry &
    Emergency, School of Stomatology, The Fourth Military Medical University, Xi'
    an 710032,China.
  • Received:2016-12-20 Online:2017-06-20 Published:2017-06-26

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摘要： 目的:观察周期性流体静压力作用下骨髓间充质干细胞(Bone Marrow Stem cells,BMSCs)细胞增殖活性及超微结构的改变。方法:贴壁法分离培养大鼠BMSCs。采用自主研发的流体静压力加载装置对大鼠BMSCs进行力学加载,加载压力为0~45 kPa、0~90 kPa,加载频率为0.1 Hz,时间1 h,连续加载2 d。对照组细胞不加压。采用CCK8法检测细胞的增殖活性,采用透射电镜观察大鼠BMSCs超微结构的变化。结果:0~45 kPa 、0~90 kPa周期性流体静压力作用下BMSCs增殖活性显著高于对照组(P<0.05),透射电镜下观察发现,0~45 kPa作用下BMSCs细胞内质网轻度扩张,胶原合成量增加。而0~90 kPa作用下细胞出现了大量胶原及凋亡小体。结论:适宜的周期性流体静压力作用下可提高细胞的增殖活性,但过高的压力刺激可诱导细胞产生凋亡。

关键词: 周期性流体静压力, 骨髓间充质干细胞, 增殖, 超微结构

Abstract: Objective: Evaluate the proliferation and ultrastructure change of rat’s BMSCs under cyclic hydrostatic pressure. Methods: BMSCs was obtained from rat by the attachment culture method. Mechanical loading of BMSCs was carried out by self-developed hydrostatic pressure loading device. The pressing pressure are 0-45kPa and 0~90kPa. BMSCs were treated with pressure 1h/d for consecutive 2 days, while the controls BMSCs were treated without pressure. Proliferation capabilities of BMSCs were detected by CCK8, while the ultrastructures of BMSCs were examined by transmission electron microscopy. Results: The proliferating activation of BMSCs under cyclic hydrostatic pressure (0-45kPa and 0-90 kPa) was higher in evidence than that of control group (P<0.05). The BMSCs treated with 0-45kPa showed mild expansion of endoplasmic reticulum, and secreted collagen around the cell. However, the BMSCs treated with 0~90 kPa showed the character of apoptosis coinciding with rich matrix. Conclusion: Appropriate measure of hydrostatic pressure promotes proliferation of BMSCs, whereas overdue hydrostatic pressure may induce apoptosis of BMSCs.

Key words: Cyclic hydrostatic pressure, BMSCs , Proliferation, Ultrastructure

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