磷酸二酯酶4D在口腔鳞状细胞癌发生发展过程中的作用研究

doi:10.13701/j.cnki.kqyxyj.2017.06.021

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (6): 663-667.DOI: 10.13701/j.cnki.kqyxyj.2017.06.021

磷酸二酯酶4D在口腔鳞状细胞癌发生发展过程中的作用研究

夏冬景^*, 黄莹莹, 李海朋, 芮航

河南南阳医学高等专科学校第一附属医院口腔科河南南阳 473058)

收稿日期:2017-01-11 出版日期:2017-06-20 发布日期:2017-06-26
通讯作者: 夏冬景,E-mail:dongjing_xia@126.com
作者简介:夏冬景(1972~ ),女,学士,主治医师,主要从事老年人牙体缺损修复的研究及治疗。

Role of Phosphodiesterase 4D in the Pathogenesis of Oral Squamous Cell Carcinoma.

XIA Dong-jing^*, HUANG Ying-ying, LI Hai-peng, RUI Hang.

Department of Stomatology, Affiliated Hospital of Nanyang Medical College, Nanyang 473058, China.

Received:2017-01-11 Online:2017-06-20 Published:2017-06-26

摘要/Abstract

摘要： 目的:探讨磷酸二酯酶4D(PDE4D)在口腔鳞状细胞癌(OSCC)发生发展过程中的作用。方法:收集53例OSCC患者标本和5种OSCC细胞株,55例正常健康标本及2种正常口腔上皮细胞作为对照,利用qRT-PCR检测其中PDE4D mRNA表达水平;分别利用CCK-8,细胞划痕实验,流式细胞术和Western blot等技术检测PDE4D抑制剂Rolipram对SSC25细胞增殖、迁移、凋亡的影响。结果:OSCC患者组织中PDE4D mRNA相对水平显著高于正常对照组;OSCC细胞株中PDE4D mRNA相对水平显著高于正常细胞组,差异有显著统计学意义(P<0.01);且分化程度越高,PDE4D mRNA相对水平越高(P<0.05);Rolipram显著抑制SSC25细胞的增殖能力和迁移能力,促进细胞凋亡(P<0.05)。结论:PDE4D在OSCC发生发展过程中起关键调控作用。

关键词: 口腔鳞状细胞癌, 磷酸二酯酶4D, 增殖, 迁移, 凋亡

Abstract: Objective: To investigate the role of phosphodiesterase 4D in the pathogenesis of oral squamous cell carcinoma (OSCC). Methods: Fifty-three OSCC patients and 5 OSCC cell lines were collected, 55 healthy adults and 2 normal oral epithelial cells were used as the control. The mRNA levels of PDE4D in these samples, the proliferation of SSC25, the migration abilities, and the cell apoptosis were examined by quantitative RT-PCR, CCK-8, wounding healing assay, and flow cytometry as well as western blot, respectively. Results: The levels of PDE4D mRNA in OSCC tissue and cell lines were significant higher than that in normal control group (P<0.01); the levels of PDE4D mRNA in OSCC tissue were positively correlated with the degree of OSCC differentiation (P<0.05); the abilities of cell proliferation and migration were significantly inhibited by Rolipram; Rolipram induced a significant apoptotic changes on SCC25 cells (P<0.05). Conclusion: PDE4D plays an important role in the OSCC pathogenesis.

Key words: Oral squamous cell carcinoma , Phosphodiesterase, 4D, Proliferation, Migration , Apoptosis

中图分类号:

R739.8

夏冬景, 黄莹莹, 李海朋, 芮航. 磷酸二酯酶4D在口腔鳞状细胞癌发生发展过程中的作用研究[J]. 口腔医学研究, 2017, 33(6): 663-667.

XIA Dong-jing, HUANG Ying-ying, LI Hai-peng, RUI Hang.. Role of Phosphodiesterase 4D in the Pathogenesis of Oral Squamous Cell Carcinoma.[J]. Journal of Oral Science Research, 2017, 33(6): 663-667.

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磷酸二酯酶4D在口腔鳞状细胞癌发生发展过程中的作用研究

Role of Phosphodiesterase 4D in the Pathogenesis of Oral Squamous Cell Carcinoma.

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