口腔医学研究

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (7): 717-720.DOI: 10.13701/j.cnki.kqyxyj.2017.07.007

• 基础研究论著 • 上一篇    下一篇

TLRs与NLRs协同促进树突状细胞的成熟

林珍, 李宇红*   

  1. 武汉大学口腔医学院口腔生物医学教育部重点实验室 湖北 武汉 430079
  • 收稿日期:2017-03-13 出版日期:2017-07-20 发布日期:2017-07-27
  • 通讯作者: 李宇红,电话:027-87686007
  • 作者简介:林珍(1990～),女,硕士,主要从事口腔牙体牙髓病学的基础研究工作。
  • 基金资助:
    国家自然科学基金(编号:81670981)

TLRs and NLRs Synergistically Promote the Maturation of Dendritic Cells.

LIN Zhen, LI Yu-hong*.   

  1. Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China.
  • Received:2017-03-13 Online:2017-07-20 Published:2017-07-27

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摘要： 目的:研究TLRs与NLRs协同促进树突状细胞成熟的效应。方法:TLRs的配体与NLRs的配体刺激小鼠DC2.4细胞24 h后用流式细胞仪检测细胞表面标记CD40、CD83、CD86、CD80与MHC-Ⅱ分子的表达。结果:单一TLRs或NLRs的配体可促进DCs表面标记分子表达的升高,并且2种配体的同时刺激可使以上标记分子的表达高于单一配体刺激。结论:TLRs与NLRs可协同促进DCs的成熟。

关键词: 树突状细胞, TLRs, NLRs, 标记分子

Abstract: Objective: To study the effect of TLRs and NLRs on the maturation of dendritic cells. Methods: The DC2.4 cells were stimulated with a single ultrapure TLR/NLR ligand or a combination of two ligands. After 24 hours, the flow cytometry was used to test the expression of CD40, CD83, CD86, CD80, and MHC-Ⅱon the surface of DCs. Results: A single TLR or NLR ligand could promote the expression of DCs surface markers, and the combined stimulation of two ligands could enhance the expression of the above markers. Conclusion: TLRs and NLRs can synergistically promote the maturation of DCs.

Key words: Dendritic cells (DCs), TLRs, NLRs, Marker molecules

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