口腔医学研究

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (7): 712-716.DOI: 10.13701/j.cnki.kqyxyj.2017.07.006

• 基础研究论著 • 上一篇    下一篇

三维打印β-TCP颌骨修复支架的生物学评价

曹帅帅1, 周苗1*, Pedro, Miranda2, 车月娟3, 陈晓明4, 李树祎1, 耿远明5, 杨晓彬1   

  1. 1. 广州医科大学附属口腔医院·广州口腔病研究所·口腔医学重点实验室 广东 广州 510140;
    2. Departamento de Ingeniería Mecánica, Energética y de los Materiales, Universidad de Extremadura, Spain 999023;
    3. 中山大学孙逸仙纪念医院麻醉科 广东 广州 510120;
    4. 广州医科大学生物医学工程系 广东 广州 511436;
    5. 南方医科大学珠江医院口腔科 广东 广州 510280
  • 收稿日期:2016-12-15 出版日期:2017-07-20 发布日期:2017-07-27
  • 通讯作者: 周苗,E-mail:zhm1000@gmail.com
  • 作者简介:曹帅帅(1990～ ),男,江苏扬州人,硕士生在读,主要从事三维打印修复颌骨缺损的研究。
  • 基金资助:
    国家自然科学基金(编号:81671029)国际口腔种植协会 (International Team for Implantology, ITI)基金(编号:881-2012)广东省科技计划项目(编号:2013B021800314)广东省科技计划项目 (编号:2015B090920002)

Biological Evaluation of Three-dimensional Printed β-TCP Scaffold for Jaw Reconstruction.

CAO Shuai-shuai1, ZHOU Miao1, MIRANDA Pedro2, CHE Yue-juan3, CHEN Xiao-ming4, LI Shu-yi1, GENG Yuan-ming5, YANG Xiao-bin1.   

  1. 1.Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou 510140, China;
    2. Departamento de Ingeniería Mecánica, Energética y de los Materiales, Universidad de Extremadura, Spain 999023;
    3. Department of Anesthesiology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China;
    4. Biomedical Engineering Department, Guangzhou Medical University, Guangzhou 511436, China;
    5. Department of Stomatology, Zhujiang Hospital, Southern Medical University Guangzhou 510280, China.
  • Received:2016-12-15 Online:2017-07-20 Published:2017-07-27

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摘要： 目的:探讨三维打印β-磷酸三钙(β-Tricalcium Phosphate, β-TCP)颌骨修复支架的生物学特性及体内成骨作用。方法:采用自动注浆技术制作β-TCP支架,将前成骨细胞(MC35T3-E1)接种在支架上,扫描电镜(SEM)观察材料结构与细胞黏附,CCK-8法检测细胞增殖,ALP法检测碱性磷酸酶活性。将2种支架复合重组人骨形成蛋白-2(recombinant human bone morphogenetic protein-2, rhBMP-2)后植入大鼠体内,发泡法制作的β-TCP支架为对照组,6周后取材行组织学观察。结果:三维打印支架具有规则多孔的立体结构,适合细胞黏附,且增殖及分化能力均高于对照组(P<0.05)。组织学显示复合rhBMP-2后三维打印支架新骨生成量高于发泡法制作的β-TCP支架(P<0.05)。结论:三维打印TCP支架生物相容性良好,复合rhBMP-2后可异位成骨。

关键词: 三维打印, 自动注浆技术, β-TCP, rhBMP-2, 骨修复

Abstract: Objective: To explore the biological characteristics and in vivo osteogenesis of β-tricalcium phosphate (β-TCP) scaffold fabricated by three-dimensional printing (3DP). Methods: β-TCP scaffolds were fabricated by robocasting. MC3T3-E1 cells were seeded onto the scaffold, then scanning electron microscope (SEM) was used to characterize the surface morphology and cells’ adhesion. The proliferation and differentiation capability of the cells were assessed by cell counting kit-8 (CCK-8) and alkaline phosphatase activities (ALP). The scaffolds incorporated with recombinant human bone morphogenetic protein-2 (rhBMP-2) were implanted into the rats, while foaming-made β-TCP scaffolds were used as control. The bone formation was observed by histological examination in 6 weeks after operation. Results: The scaffold possessed a well-defined porous stereo-structure. The cell proliferation and differentiation capacity of the 3DP scaffold were significantly higher than those in control group (P<0.05). Histological examination showed higher new bone formation of the 3DP β-TCP than foaming-made scaffold incorporated with rhBMP-2 (P<0.05). Conclusion: 3DP β-TCP scaffold showed an excellent biocompatibility and ectopic osteogenesis.

Key words: Three-dimensional printing, Robocasting , β-TCP rh, BMP-2 , Bone repair

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