多孔双相磷酸钙陶瓷修复骨质疏松症大鼠颅骨极量缺损的实验研究

doi:10.13701/j.cnki.kqyxyj.2019.04.017

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (4): 377-381.DOI: 10.13701/j.cnki.kqyxyj.2019.04.017

多孔双相磷酸钙陶瓷修复骨质疏松症大鼠颅骨极量缺损的实验研究

彭双麟¹, 姚志浩¹, 罗道文¹, 杨双林¹, 李勇¹, 肖金刚^1,2,3*

1. 西南医科大学口腔医学院四川泸州 646000;
2. 西南医科大学附属医院口腔颌面外科四川泸州 646000;
3. 西南医科大学口颌面修复重建和再生实验室四川泸州 646000

收稿日期:2018-09-12 出版日期:2019-04-28 发布日期:2019-04-23
通讯作者: 肖金刚,E-mail:drxiaojingang@163.com
作者简介:彭双麟(1995~ ),男,四川泸县人,硕士在读,主要从事口腔颌面外科临床与基础研究。
基金资助:
国家自然科学基金(编号:81870746,81371125);四川省科技厅项目(编号:2014JY0044);泸州市人民政府-西南医科大学科技战略合作项目(编号:2015LZCYD-S05[2/12])

Experimental Study on Repair of Critical-size Calvarial Defects in Osteoporotic Rats by Biphasic Calcium Phosphate Ceramic Scaffold

PENG Shuang-lin¹, YAO Zhi-hao¹, LUO Dao-wen¹, YANG Shuang-lin¹, LI Yong¹, XIAO Jin-gang^1,2,3*

1. School of Stomatology, Southwest Medical University, Luzhou 646000, China;
2. Department of Oral and Maxillofacial Surgery, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, China;
3. Orofacial Reconstruction and Regeneration Laboratory, Southwest Medical University, Luzhou 646000, China

Received:2018-09-12 Online:2019-04-28 Published:2019-04-23

摘要/Abstract

摘要： 目的: 探讨多孔双相磷酸钙陶瓷支架(BCP)对骨质疏松症SD大鼠颅骨极量缺损的修复效果。方法: 建立骨质疏松症SD大鼠颅骨极量缺损模型,运用BCP修复颅骨极量缺损,分为4组,分别是Ctrl组,OP组,Ctrl+BCP组,OP+BCP组,8周后处死大鼠,应用Micro-CT、HE和Masson染色检测骨形成差异。结果: Ctrl组和OP组未见明显新生骨组织;Ctrl+BCP组和OP+BCP组可见新生骨组织,骨质疏松症组(OP+BCP组)新生骨组织明显少于非骨质疏松组(Ctrl+BCP组)。结论: BCP对骨质疏松症SD大鼠颅骨极量缺损具有一定的修复作用,可作为骨质疏松症大鼠颅骨极量缺损修复的支架材料,但修复效果弱于正常SD大鼠。

关键词: 双相磷酸钙(BCP), 骨质疏松, 颅骨极量缺损, 骨修复

Abstract: Objective: To study the effect of the biphasic calcium phosphate ceramic scaffold (BCP) on the repair of critical-size calvarial defects in SD rats with osteoporosis. Methods: SD rats were made into critical-size calvarial defects with osteoporosis. BCP was used to repair the defect of the skull. The rats were divided into four groups, i.e. the Ctrl group, the OP group, the Ctrl+BCP group, and the OP+BCP group. Rats in each group were sacrificed 8 weeks later and the differences in bone formation were detected by imaging findings (Micro-CT) and histological manifestations (Masson staining and hematoxylin-eosin staining). Results: No obvious new bone was found in the Ctrl group and the OP group. New bone can be found in the Ctrl+BCP group and the OP+BCP group, and the new bone in the OP+BCP group was significantly less than that in the non-osteoporosis group (the Ctrl+BCP group). Conclusion: BCP can be used as the scaffold for the repair of critical-size calvarial defects in SD rats with osteoporosis, but the repair effect is weaker than that of normal SD rats.

Key words: Calcium phosphate biphasic (BCP), Osteoporosis, Critical-size calvarial defects, Bone regeneration

彭双麟, 姚志浩, 罗道文, 杨双林, 李勇, 肖金刚. 多孔双相磷酸钙陶瓷修复骨质疏松症大鼠颅骨极量缺损的实验研究[J]. 口腔医学研究, 2019, 35(4): 377-381.

PENG Shuang-lin, YAO Zhi-hao, LUO Dao-wen, YANG Shuang-lin, LI Yong, XIAO Jin-gang. Experimental Study on Repair of Critical-size Calvarial Defects in Osteoporotic Rats by Biphasic Calcium Phosphate Ceramic Scaffold[J]. Journal of Oral Science Research, 2019, 35(4): 377-381.

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多孔双相磷酸钙陶瓷修复骨质疏松症大鼠颅骨极量缺损的实验研究

Experimental Study on Repair of Critical-size Calvarial Defects in Osteoporotic Rats by Biphasic Calcium Phosphate Ceramic Scaffold

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